[Objective] The study was conducted to establish a high efficient micropropagation system for Rhododendron Brachycarpum D. Don and realize the high efficiency in vitro micropropagation of R. brachycarpum. [ Method ] Y...[Objective] The study was conducted to establish a high efficient micropropagation system for Rhododendron Brachycarpum D. Don and realize the high efficiency in vitro micropropagation of R. brachycarpum. [ Method ] Young stems of R. brachycarpum were used as explants, suitable medium compositions for axillary bud growing and rooting were screened through uniform design exPeriments. [ Result] MS (modified) + IAA 0,15 mg/ L + IBA 0.30 mg/L + GA3 3.00 mg/L was the most suitable medium with the regeneration rate of 92%. Stems each with one node were cut from regenerated shoots and cultured for propagation, the proliferative multiple was over 45 within one culture period of 35 d. [ Conclusion] High efficient micropropagation system of R. brachycarpum has been successfully established, which provides some basis for development and utilization and industrial seedling growth of the alpine rhododendron in Changbai Mountain northeast China.展开更多
基金Supported by Funded Project of Science and Technology Department in Jilin Province(200705C05)Natural Science Funds of Tonghua Normal University (XS060074)~~
文摘[Objective] The study was conducted to establish a high efficient micropropagation system for Rhododendron Brachycarpum D. Don and realize the high efficiency in vitro micropropagation of R. brachycarpum. [ Method ] Young stems of R. brachycarpum were used as explants, suitable medium compositions for axillary bud growing and rooting were screened through uniform design exPeriments. [ Result] MS (modified) + IAA 0,15 mg/ L + IBA 0.30 mg/L + GA3 3.00 mg/L was the most suitable medium with the regeneration rate of 92%. Stems each with one node were cut from regenerated shoots and cultured for propagation, the proliferative multiple was over 45 within one culture period of 35 d. [ Conclusion] High efficient micropropagation system of R. brachycarpum has been successfully established, which provides some basis for development and utilization and industrial seedling growth of the alpine rhododendron in Changbai Mountain northeast China.