MtDNA was successfully extracted from 31 ancient individual bones at the tombs of Turfan basin and Lubunouer. Through four overlapping primers, we got 30 nucleotide sequences of 363 bp length. Intermediate nucleotide ...MtDNA was successfully extracted from 31 ancient individual bones at the tombs of Turfan basin and Lubunouer. Through four overlapping primers, we got 30 nucleotide sequences of 363 bp length. Intermediate nucleotide diversity, and genetic distances, as well as mean pairwise variance, are compatible with ancient Turfan mtDNA pool being an admixture of eastern Asian and European lineages. Such phenomenon shows that prior to the time of ancient Turfan, an ancient mingling of Euro-Asian population in Turfan basin had existed. The ancient Luobunuoer was the relic of Xinjiang that was found in the earliest time and at the farthest orient. The mtDNA sequences of ancient Luobunuoer are consisted by European lineage completely, no trace of mixture with Asian lineage. From nucleotide diversity, and genetic distances and mean pairwise variance, it has close relationship with European populations.展开更多
山普拉墓地位于新疆维吾尔自治区和田地区洛浦县城西南14km处,14C测定该墓地年代为公元前217至公元283年,属于古代新疆的于阗国遗存.实验通过4对套叠引物对山普拉古代居民线粒体基因组的高可变一区(364bp)进行扩增和测序,同时选择编码区...山普拉墓地位于新疆维吾尔自治区和田地区洛浦县城西南14km处,14C测定该墓地年代为公元前217至公元283年,属于古代新疆的于阗国遗存.实验通过4对套叠引物对山普拉古代居民线粒体基因组的高可变一区(364bp)进行扩增和测序,同时选择编码区6个位点做限制性片段长度分析(RFLP,Restriction Fragment Length Polymorphism).从16例山普拉个体中得到了13个真实可靠的线粒体DNA数据.对线粒体DNA单倍型类群分析结果表明,山普拉古代居民同时具有西部欧亚大陆和东部欧亚大陆特有的单倍性类群,是一混合人群.多维尺度分析以及U3亚型的中介网络图分析表明,山普拉古代人群与伊朗人群及奥塞梯人群有一定母系遗传联系.展开更多
文摘MtDNA was successfully extracted from 31 ancient individual bones at the tombs of Turfan basin and Lubunouer. Through four overlapping primers, we got 30 nucleotide sequences of 363 bp length. Intermediate nucleotide diversity, and genetic distances, as well as mean pairwise variance, are compatible with ancient Turfan mtDNA pool being an admixture of eastern Asian and European lineages. Such phenomenon shows that prior to the time of ancient Turfan, an ancient mingling of Euro-Asian population in Turfan basin had existed. The ancient Luobunuoer was the relic of Xinjiang that was found in the earliest time and at the farthest orient. The mtDNA sequences of ancient Luobunuoer are consisted by European lineage completely, no trace of mixture with Asian lineage. From nucleotide diversity, and genetic distances and mean pairwise variance, it has close relationship with European populations.
文摘山普拉墓地位于新疆维吾尔自治区和田地区洛浦县城西南14km处,14C测定该墓地年代为公元前217至公元283年,属于古代新疆的于阗国遗存.实验通过4对套叠引物对山普拉古代居民线粒体基因组的高可变一区(364bp)进行扩增和测序,同时选择编码区6个位点做限制性片段长度分析(RFLP,Restriction Fragment Length Polymorphism).从16例山普拉个体中得到了13个真实可靠的线粒体DNA数据.对线粒体DNA单倍型类群分析结果表明,山普拉古代居民同时具有西部欧亚大陆和东部欧亚大陆特有的单倍性类群,是一混合人群.多维尺度分析以及U3亚型的中介网络图分析表明,山普拉古代人群与伊朗人群及奥塞梯人群有一定母系遗传联系.
