细基江蓠(Gracilaria tenuistipitata Chang et Xia)是江蓠属的一个重要物种,其繁枝变种体(G.tenuistiptata vat.liui Zhang et Xia)具有重要的养殖经济价值。在我国沿海地区,主要分布在海南岛北部沿岸的潮间带海区。鉴于该变种体具有...细基江蓠(Gracilaria tenuistipitata Chang et Xia)是江蓠属的一个重要物种,其繁枝变种体(G.tenuistiptata vat.liui Zhang et Xia)具有重要的养殖经济价值。在我国沿海地区,主要分布在海南岛北部沿岸的潮间带海区。鉴于该变种体具有生长较快、可营养枝体进行繁殖、能适应在人工裁培条件下的塘池养殖、管理操作技术难度不大、经济效益较好、适合群众性各种规模放养等特点。展开更多
This study on dynamic changes of culture color, astaxanthin and chlorophylls, inorganic N including N NO - 3, N NO - 2 and N NH + 4 in batch culture of Haematococcus pluvialis exposed to different additive nitrate con...This study on dynamic changes of culture color, astaxanthin and chlorophylls, inorganic N including N NO - 3, N NO - 2 and N NH + 4 in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentration showed (1) ast/chl ratio was over 0.8 for brown and red algae, but was usually less than 0.5 for green and yellow algae; (2) N NO - 3, in general, was unstable and decreased, except for a small unexpected increase in nitrate enriched treatment groups; (3) measurable amounts of N NO - 2 and N NH + 4 were observed respectively with three change modes although no external nitrite and ammonia were added into the culture; (4) a non linear correlation between ast/chl ratio (or color) changes and the levels of N NO - 3 , N NO - 2 , N NH + 4 in H. pluvialis culture; (5) up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown (or red) when N NO - 3, N NO - 2 and N NH + 4 fluctuated around 30, 5, 5 μmol/L respectively; (6) existence of three dynamic modes of N NO - 3, N NO - 2 and N NH + 4 changes, obviously associated with initial external nitrate; (7) the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L; and (8) 0.5-10 mmol/L of nitrate was theoretically conducive to cell growth in batch culture.展开更多
The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine g...The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine genus of the Eustigmatophyceae, were carried out in Qingdao and Sigapore with nature seawater. Based on the results obtained, a optimum model good for culturing this unicellular alga was suggested as follows: 120mg/L urea, 6mg/L KH2PO4, 1.5 mg/L Ferric citrate, 0.05 mg/L ZnSO4, 0.01 mg/L CuSO4, 0.1 mg/L NaMoO3, 0.02 mg/L CoCl2, 0.5 mg/L HBO3, 1.0 mg/L MnSO4,25‰～30‰NaCl, continuous illumination indoor or suitable strong light intensity as well as a contant temperature between 25～30℃, or varied temperature between 25～35℃, specific growth rate at 10% ～15%.3.6×1012 to 1.2×1011 cells /L of dense culture of Nannochloropsis salina were achieved by using 10L of tubular coiled photobioreactor,60L, 200L, 500L and 1600 L of flat glass photoreactors, respectively, which was several hundred-fold higher than the cell density of traditional microalgal culture in aquaculture. Specific growth rate, negative correlation with cell density, affected the biomass output in Nannochloropsis salina culture system. 10%～15% of specific growth rate was suggested in application for maximal production.展开更多
文摘细基江蓠(Gracilaria tenuistipitata Chang et Xia)是江蓠属的一个重要物种,其繁枝变种体(G.tenuistiptata vat.liui Zhang et Xia)具有重要的养殖经济价值。在我国沿海地区,主要分布在海南岛北部沿岸的潮间带海区。鉴于该变种体具有生长较快、可营养枝体进行繁殖、能适应在人工裁培条件下的塘池养殖、管理操作技术难度不大、经济效益较好、适合群众性各种规模放养等特点。
文摘This study on dynamic changes of culture color, astaxanthin and chlorophylls, inorganic N including N NO - 3, N NO - 2 and N NH + 4 in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentration showed (1) ast/chl ratio was over 0.8 for brown and red algae, but was usually less than 0.5 for green and yellow algae; (2) N NO - 3, in general, was unstable and decreased, except for a small unexpected increase in nitrate enriched treatment groups; (3) measurable amounts of N NO - 2 and N NH + 4 were observed respectively with three change modes although no external nitrite and ammonia were added into the culture; (4) a non linear correlation between ast/chl ratio (or color) changes and the levels of N NO - 3 , N NO - 2 , N NH + 4 in H. pluvialis culture; (5) up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown (or red) when N NO - 3, N NO - 2 and N NH + 4 fluctuated around 30, 5, 5 μmol/L respectively; (6) existence of three dynamic modes of N NO - 3, N NO - 2 and N NH + 4 changes, obviously associated with initial external nitrate; (7) the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L; and (8) 0.5-10 mmol/L of nitrate was theoretically conducive to cell growth in batch culture.
文摘The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine genus of the Eustigmatophyceae, were carried out in Qingdao and Sigapore with nature seawater. Based on the results obtained, a optimum model good for culturing this unicellular alga was suggested as follows: 120mg/L urea, 6mg/L KH2PO4, 1.5 mg/L Ferric citrate, 0.05 mg/L ZnSO4, 0.01 mg/L CuSO4, 0.1 mg/L NaMoO3, 0.02 mg/L CoCl2, 0.5 mg/L HBO3, 1.0 mg/L MnSO4,25‰～30‰NaCl, continuous illumination indoor or suitable strong light intensity as well as a contant temperature between 25～30℃, or varied temperature between 25～35℃, specific growth rate at 10% ～15%.3.6×1012 to 1.2×1011 cells /L of dense culture of Nannochloropsis salina were achieved by using 10L of tubular coiled photobioreactor,60L, 200L, 500L and 1600 L of flat glass photoreactors, respectively, which was several hundred-fold higher than the cell density of traditional microalgal culture in aquaculture. Specific growth rate, negative correlation with cell density, affected the biomass output in Nannochloropsis salina culture system. 10%～15% of specific growth rate was suggested in application for maximal production.
