目的:探讨干燥综合征(sjgren's syndrome,SS)型干眼患者生命质量。方法:采用36条目简明量表(SF-36)和视功能损害眼病患者生存质量(SQOL-DVI)对42例SS型干眼患者的生命质量(qualityofLife,QL)进行评价研究。结果:SS型干眼患者全面健...目的:探讨干燥综合征(sjgren's syndrome,SS)型干眼患者生命质量。方法:采用36条目简明量表(SF-36)和视功能损害眼病患者生存质量(SQOL-DVI)对42例SS型干眼患者的生命质量(qualityofLife,QL)进行评价研究。结果:SS型干眼患者全面健康相关生存质量各维度均下降,并与视觉相关生存质量具相关性,尤其在躯体功能对角色功能的影响(role limitation due to physical problem,RP)、情绪对角色功能的影响(role limitation due to emotional problem,RE)、健康总体自评(general health,GH)和社会功能(social functioning,SF)方面尤为突出。结论:与其他类型干眼患者相比,SS型干眼患者生存质量明显降低。应该重视SS型干眼患者的社会心理状况及相关治疗,结合病因个体化施治,为评价各种治疗措施对SS型干眼患者生存质量的影响提供依据。展开更多
目的：观察Nd：YAG泪道激光再造成形联合泪道逆行置管术治疗泪道阻塞的临床疗效及影响手术效果的因素。 方法：选取20例23眼泪道阻塞患者行Nd： YAG激光再造成形联合逆行泪道引流管留置3 mo ，拔管后随访3～12 mo ，观察术前患侧鼻腔...目的：观察Nd：YAG泪道激光再造成形联合泪道逆行置管术治疗泪道阻塞的临床疗效及影响手术效果的因素。 方法：选取20例23眼泪道阻塞患者行Nd： YAG激光再造成形联合逆行泪道引流管留置3 mo ，拔管后随访3～12 mo ，观察术前患侧鼻腔情况，术中鼻腔损伤程度、术后流泪和流脓情况、泪道功能恢复及并发症情况。 结果：末次随访时，经此方法治疗各种泪道阻塞的患者治愈19眼（82．6％）；有效2眼（8．7％）；无效2眼（8．7％），总有效率91．3％。其中泪小管阻塞者有效率100％（2/2），泪总管阻塞者有效率100％（15/15），鼻泪管阻塞者有效率66．7％（2/3），泪小管合并鼻泪管阻塞者有效率66．7％（2/3）。1眼术后2wk左右有轻微眼部刺激症状，3眼出现泪小管周围组织红肿不适，2眼出现泪小点撕裂，无1例患者出现假道及泪道插管滑脱，余未见其他并发症发生。 结论：泪道激光再造成形联合逆行泪道引流管留置术治疗泪道阻塞是一种有效的治疗方法。展开更多
This study aimed to modify the mixed and purified culture of rat retinal ganglion cells（RGCs） in vitro.The retinae of 1-3 day old Sprague-Dawley（SD） rats were separated bluntly into two layers：inner layer and out...This study aimed to modify the mixed and purified culture of rat retinal ganglion cells（RGCs） in vitro.The retinae of 1-3 day old Sprague-Dawley（SD） rats were separated bluntly into two layers：inner layer and outer layer,under a surgical microscope.Retinal cells isolated from different layers（inner layer,outer layer and whole retinal tissue） by using enzyme dissociation method were cultured in F12/DMEM medium containing 15% FBS.After 3-day culture,the RGCs in the retinal cells obtained from mixed culture of inner,outer,and whole retinal tissue were identified by immunocytochemical staining of Thy-1.1,and the rate of RGCs to retinal cells（RGCs%） was calculated.Two monoclonal antibodies,anti-macrophages/granulocytes（OX-41） against rat macrophage and antibody against rat Thy-1.1（OX-7）,were used to purify RGCs by either a conventional or modified two-stepped immunopanning procedure（purification in situ）.Purified RGCs were seeded at different cell density and cultured in F12/DMEM medium containing 15% FBS.Immunocytochemical staining for Thy-1.1,MTT,and PI-Hoechst33342 fluorescence imaging were used to identify the purity and the viability of RGCs in purified culture of RGCs.The results showed：（1） Immunocytochemistry of different retinal tissue layers culture revealed that the RGCs% was（19.9±1.2）%,（0.5±0.2）%,and（6.2±1.7）% respectively in the mixed culture of inner,outer,and whole retinal tissue,with differences being significant（P0.05）;（2） fluorescent double staining of Hoechst33342 and PI indicated that with the same RGCs%,RGCs obtained from purification in situ grew well with more neurite outgrowth than those by the conventional two-stepped immunopanning method;（3） the viability of purified RGCs seeded at high density was in-creased and the cells developed complex intercellular networks.The viability of RGCs was declined with the decreasing seeding density,and most cells presented round or oval in shape with thin neurites.It was concluded that：（1） RGCs% in the inner layer retina was higher than that in the outer layer retina;（2） RGCs obtained by in situ purification had more neurite outgrowth and lower mortality than those by conventional two-stepped immunopanning procedure;（3） the viability of purified RGCs could be increased by increasing cell seeding density to some extent.展开更多
文摘目的:探讨干燥综合征(sjgren's syndrome,SS)型干眼患者生命质量。方法:采用36条目简明量表(SF-36)和视功能损害眼病患者生存质量(SQOL-DVI)对42例SS型干眼患者的生命质量(qualityofLife,QL)进行评价研究。结果:SS型干眼患者全面健康相关生存质量各维度均下降,并与视觉相关生存质量具相关性,尤其在躯体功能对角色功能的影响(role limitation due to physical problem,RP)、情绪对角色功能的影响(role limitation due to emotional problem,RE)、健康总体自评(general health,GH)和社会功能(social functioning,SF)方面尤为突出。结论:与其他类型干眼患者相比,SS型干眼患者生存质量明显降低。应该重视SS型干眼患者的社会心理状况及相关治疗,结合病因个体化施治,为评价各种治疗措施对SS型干眼患者生存质量的影响提供依据。
文摘目的：观察Nd：YAG泪道激光再造成形联合泪道逆行置管术治疗泪道阻塞的临床疗效及影响手术效果的因素。 方法：选取20例23眼泪道阻塞患者行Nd： YAG激光再造成形联合逆行泪道引流管留置3 mo ，拔管后随访3～12 mo ，观察术前患侧鼻腔情况，术中鼻腔损伤程度、术后流泪和流脓情况、泪道功能恢复及并发症情况。 结果：末次随访时，经此方法治疗各种泪道阻塞的患者治愈19眼（82．6％）；有效2眼（8．7％）；无效2眼（8．7％），总有效率91．3％。其中泪小管阻塞者有效率100％（2/2），泪总管阻塞者有效率100％（15/15），鼻泪管阻塞者有效率66．7％（2/3），泪小管合并鼻泪管阻塞者有效率66．7％（2/3）。1眼术后2wk左右有轻微眼部刺激症状，3眼出现泪小管周围组织红肿不适，2眼出现泪小点撕裂，无1例患者出现假道及泪道插管滑脱，余未见其他并发症发生。 结论：泪道激光再造成形联合逆行泪道引流管留置术治疗泪道阻塞是一种有效的治疗方法。
基金supported by a grant from National Natural Sciences Foundation of China(No.30700922)
文摘This study aimed to modify the mixed and purified culture of rat retinal ganglion cells（RGCs） in vitro.The retinae of 1-3 day old Sprague-Dawley（SD） rats were separated bluntly into two layers：inner layer and outer layer,under a surgical microscope.Retinal cells isolated from different layers（inner layer,outer layer and whole retinal tissue） by using enzyme dissociation method were cultured in F12/DMEM medium containing 15% FBS.After 3-day culture,the RGCs in the retinal cells obtained from mixed culture of inner,outer,and whole retinal tissue were identified by immunocytochemical staining of Thy-1.1,and the rate of RGCs to retinal cells（RGCs%） was calculated.Two monoclonal antibodies,anti-macrophages/granulocytes（OX-41） against rat macrophage and antibody against rat Thy-1.1（OX-7）,were used to purify RGCs by either a conventional or modified two-stepped immunopanning procedure（purification in situ）.Purified RGCs were seeded at different cell density and cultured in F12/DMEM medium containing 15% FBS.Immunocytochemical staining for Thy-1.1,MTT,and PI-Hoechst33342 fluorescence imaging were used to identify the purity and the viability of RGCs in purified culture of RGCs.The results showed：（1） Immunocytochemistry of different retinal tissue layers culture revealed that the RGCs% was（19.9±1.2）%,（0.5±0.2）%,and（6.2±1.7）% respectively in the mixed culture of inner,outer,and whole retinal tissue,with differences being significant（P0.05）;（2） fluorescent double staining of Hoechst33342 and PI indicated that with the same RGCs%,RGCs obtained from purification in situ grew well with more neurite outgrowth than those by the conventional two-stepped immunopanning method;（3） the viability of purified RGCs seeded at high density was in-creased and the cells developed complex intercellular networks.The viability of RGCs was declined with the decreasing seeding density,and most cells presented round or oval in shape with thin neurites.It was concluded that：（1） RGCs% in the inner layer retina was higher than that in the outer layer retina;（2） RGCs obtained by in situ purification had more neurite outgrowth and lower mortality than those by conventional two-stepped immunopanning procedure;（3） the viability of purified RGCs could be increased by increasing cell seeding density to some extent.
