The effects of coxsackievirus B3 infection on induction of ICAM-1 in cardiac myocytes and on Iymphocyte-myocyte adhesion

objective:To observe the effects of coxsackievirus B3(CoxB3) infection on-induction of intercellu lar adhesion molecule-1 (ICAM-1) in cardiac myocytes and on lymphocyte-myocyte adhesion. Metkods: ICAM 1 expression on cultured neonatal rat cardiac myocytes infected by CoxB3 was determined through enzyme linked immunosorbent assay (ELISA) analysis. Lymphocyte-myocyte adhesion was observed with adherence assay and monoclonal antibodies to adhesion molecule inhibition. Results:ICAM-1 expression on cardiac my ocytes was markedly induced after exposure to CoxB3 100 or 200 TCID50/ml infectious dose for 24~48 h (P<0. 05). Adhesion of rat activated lymphocytes to myocytes was stimulated by CoxB3 infection,and the effects of CoxB3 infection on adherence was significantly inhibited by anti-lymphocyte function antigen-1 (LFA-12)monoclonal antibodies (inhibition rate 40. 6% and 26. 45 % respectively). Couclusion:CoxB3 infec tion promotes lymphocyte-myocyte adhesion through inducing ICAAM-1 expression on cardiac myocytes.

初中英语情景教学法实效性研究

随着我国经济的发展逐渐走向全球化,英语的地位和作用得到了进一步提高,同时,也对英语课堂教学提出了更高的要求。目前,国内的英语教学正如火如荼地开展着,各种创新的英语教学方式让初中英语教学收到了良好的效果。在此,情景教学法一直被广泛应用于初中英语教学中,在英语课堂教学中起到了非常重要的作用。实践证明,在初中英语教学中采用情景教学法能够有效地提升教学的质量和学生的积极性,并增强学生的英语综合运用能力。本文简要分析了初中英语课堂教学中存在的问题,并提出了情景教学法中几种有实效性的方法,希望能给读者一些帮助。