The expression of three protooncogenes (c-myc, c-fos, N-ras) in.the primary cells from 53 cases of leukemia as well as peripheral WBC from 8 normal individuals was studied. Semiquantitative analysis of mRNA levels of ...The expression of three protooncogenes (c-myc, c-fos, N-ras) in.the primary cells from 53 cases of leukemia as well as peripheral WBC from 8 normal individuals was studied. Semiquantitative analysis of mRNA levels of the protooncogenes was carried out by Quick-blot. The results showed that (1) c-myc oncogene was slightly expressed and Nras was marginally expressed, whereas the expression of the c-fos was undetectable in the peripheral leucocytes of 8 normal individuals; (2) the c-myc was obviously expressed in almost all leukemic cells irrespective of the cell types, while N-ras and c-fos were variable expressed; (3) the c-fos was expressed in all 4 cases of M4; (4) the c-myc transcript was detected but the N-ras and c-fos were not in 4 chronic leukemic cases; (5) the relationship between protooncogene expression and state of leukemia or after chemotherapy was also analysed.展开更多
A human primary gastric cancer tissue （adenocarcinoma Ⅱ—Ⅲ） was transplanted into nude mice （SWISS/DF, nu/nu）. It has been transfered for 8 generations at 56 sites in 28 nude mice with transplanting rate of 100%...A human primary gastric cancer tissue （adenocarcinoma Ⅱ—Ⅲ） was transplanted into nude mice （SWISS/DF, nu/nu）. It has been transfered for 8 generations at 56 sites in 28 nude mice with transplanting rate of 100%. The transplanted tumor is designated as transplanted human primary gastric cancer-1 in nude mice （THPGC-1）. The growth rate of THPGC-1 is rather rapid and the size of transplanted tumor reaches 1cm<sup>2</sup> in 4—5 weeks after transfer. The morphology and histochemistry of the original tumor were retained well in the initial and serial transplanted tumors. THPGC-1 could secrete carcinoembryonic antigen （CEA）. After intravenous or intraperitoneal injection of <sup>131</sup>Ⅰ-antiCEA monoclonal antibody into the THPGC-1 bearing nude mice, the radiolabeled antibody was concentrated and localized in the tumor as shown by γ-camera analysis. Similar pattern of lactate dehydrogenase isoenzyme was observed both in primary gastric cancer tissue and THPGC-1 tissue. Chromosomal examination revealed that THPGC-1 was human aneuploid ones. Southern blot analysis showed that the pattern of repetitive DNA bands and the structure of 28S rDNA, c-H-ras and c-myc genes in THPGC-1 were identical to the original primary gastric cancer DNA. The results suggest that THPGC-1 be a reliable model for the research of the molecular biology of cancer cells and experimantal gastric cancer diagnosis and treatment.展开更多
文摘The expression of three protooncogenes (c-myc, c-fos, N-ras) in.the primary cells from 53 cases of leukemia as well as peripheral WBC from 8 normal individuals was studied. Semiquantitative analysis of mRNA levels of the protooncogenes was carried out by Quick-blot. The results showed that (1) c-myc oncogene was slightly expressed and Nras was marginally expressed, whereas the expression of the c-fos was undetectable in the peripheral leucocytes of 8 normal individuals; (2) the c-myc was obviously expressed in almost all leukemic cells irrespective of the cell types, while N-ras and c-fos were variable expressed; (3) the c-fos was expressed in all 4 cases of M4; (4) the c-myc transcript was detected but the N-ras and c-fos were not in 4 chronic leukemic cases; (5) the relationship between protooncogene expression and state of leukemia or after chemotherapy was also analysed.
文摘A human primary gastric cancer tissue （adenocarcinoma Ⅱ—Ⅲ） was transplanted into nude mice （SWISS/DF, nu/nu）. It has been transfered for 8 generations at 56 sites in 28 nude mice with transplanting rate of 100%. The transplanted tumor is designated as transplanted human primary gastric cancer-1 in nude mice （THPGC-1）. The growth rate of THPGC-1 is rather rapid and the size of transplanted tumor reaches 1cm<sup>2</sup> in 4—5 weeks after transfer. The morphology and histochemistry of the original tumor were retained well in the initial and serial transplanted tumors. THPGC-1 could secrete carcinoembryonic antigen （CEA）. After intravenous or intraperitoneal injection of <sup>131</sup>Ⅰ-antiCEA monoclonal antibody into the THPGC-1 bearing nude mice, the radiolabeled antibody was concentrated and localized in the tumor as shown by γ-camera analysis. Similar pattern of lactate dehydrogenase isoenzyme was observed both in primary gastric cancer tissue and THPGC-1 tissue. Chromosomal examination revealed that THPGC-1 was human aneuploid ones. Southern blot analysis showed that the pattern of repetitive DNA bands and the structure of 28S rDNA, c-H-ras and c-myc genes in THPGC-1 were identical to the original primary gastric cancer DNA. The results suggest that THPGC-1 be a reliable model for the research of the molecular biology of cancer cells and experimantal gastric cancer diagnosis and treatment.
