目的探究脯氨酰4-羟化酶α-3(P4HA3)基因在结直肠癌(CRC)的表达及与不良预后的关系。方法从The Cancer Genome Atlas(TCGA)数据库获取CRC基因数据,包含647例CRC患者肿瘤组织和51例癌旁组织。利用R软件对P4HA3表达及配对数据可视化分析...目的探究脯氨酰4-羟化酶α-3(P4HA3)基因在结直肠癌(CRC)的表达及与不良预后的关系。方法从The Cancer Genome Atlas(TCGA)数据库获取CRC基因数据,包含647例CRC患者肿瘤组织和51例癌旁组织。利用R软件对P4HA3表达及配对数据可视化分析。卡方检验分析P4HA3表达与CRC患者临床特征关系。利用R软件survival包对P4HA3进行生存分析。利用基因表达谱交互分析(GEPIA)数据库验证TCGA数据库CRC中P4HA3的表达。通过基因集富集分析(GSEA)对P4HA3进行京都基因与基因组百科全科(KEGG)通路富集分析。采用免疫组化染色检测30例CRC患者手术切除的肿瘤和癌旁组织蜡块样本中的P4HA3蛋白表达。结果R软件分析显示,与癌旁组织比较,P4HA3在CRC组织高表达(P<0.01)。卡方检验显示,P4HA3表达与CRC患者Stage、T和N分期密切相关(P<0.05)。生存分析显示,与低表达P4HA3患者比较,P4HA3高表达CRC患者的生存时间明显缩短(P<0.05)。通过GEPIA数据库分析验证了P4HA3基因与CRC不良预后相关(P<0.05)。进一步GSEA通路富集分析结果显示,P4HA3基因富集到的信号通路与多种恶性肿瘤的发生发展有关(FDR<0.05)。最后,免疫组化检测验证了P4HA3蛋白在CRC组织中高表达。结论基因P4HA3与CRC的进展及不良预后相关,P4HA3有望成为CRC患者新的治疗靶点。展开更多
Based on the B-spline basis method, the properties of the helium atom confined inside an endohedral environment,such as buckminster fullerene, are studied. In our calculations, the endohedral environment is a paraboli...Based on the B-spline basis method, the properties of the helium atom confined inside an endohedral environment,such as buckminster fullerene, are studied. In our calculations, the endohedral environment is a parabolic potential well. In this situation, the phenomenon of 'mirror collapse' is exhibited for energy levels of a confined helium atom. The 'giant resonance' of oscillator strength of the dipole transition emerges with the variation of depth of the confining well. The physical mechanisms of these phenomena are analyzed in this paper.展开更多
文摘目的探究脯氨酰4-羟化酶α-3(P4HA3)基因在结直肠癌(CRC)的表达及与不良预后的关系。方法从The Cancer Genome Atlas(TCGA)数据库获取CRC基因数据,包含647例CRC患者肿瘤组织和51例癌旁组织。利用R软件对P4HA3表达及配对数据可视化分析。卡方检验分析P4HA3表达与CRC患者临床特征关系。利用R软件survival包对P4HA3进行生存分析。利用基因表达谱交互分析(GEPIA)数据库验证TCGA数据库CRC中P4HA3的表达。通过基因集富集分析(GSEA)对P4HA3进行京都基因与基因组百科全科(KEGG)通路富集分析。采用免疫组化染色检测30例CRC患者手术切除的肿瘤和癌旁组织蜡块样本中的P4HA3蛋白表达。结果R软件分析显示,与癌旁组织比较,P4HA3在CRC组织高表达(P<0.01)。卡方检验显示,P4HA3表达与CRC患者Stage、T和N分期密切相关(P<0.05)。生存分析显示,与低表达P4HA3患者比较,P4HA3高表达CRC患者的生存时间明显缩短(P<0.05)。通过GEPIA数据库分析验证了P4HA3基因与CRC不良预后相关(P<0.05)。进一步GSEA通路富集分析结果显示,P4HA3基因富集到的信号通路与多种恶性肿瘤的发生发展有关(FDR<0.05)。最后,免疫组化检测验证了P4HA3蛋白在CRC组织中高表达。结论基因P4HA3与CRC的进展及不良预后相关,P4HA3有望成为CRC患者新的治疗靶点。
基金supported by the National Natural Science Foundation of China(Grant No.11274246.)
文摘Based on the B-spline basis method, the properties of the helium atom confined inside an endohedral environment,such as buckminster fullerene, are studied. In our calculations, the endohedral environment is a parabolic potential well. In this situation, the phenomenon of 'mirror collapse' is exhibited for energy levels of a confined helium atom. The 'giant resonance' of oscillator strength of the dipole transition emerges with the variation of depth of the confining well. The physical mechanisms of these phenomena are analyzed in this paper.
