[Objective] The paper aimed to reveal the genetic diversity of large and medium-sized donkey breeds of China.[Method] The genetic polymorphisms of 24 microsatellites in the eight large and medium-sized donkey breeds w...[Objective] The paper aimed to reveal the genetic diversity of large and medium-sized donkey breeds of China.[Method] The genetic polymorphisms of 24 microsatellites in the eight large and medium-sized donkey breeds were studied by means of PCR,polyacrylamide gel electrophoresis and silver staining.[Result] The microsatellite of NVHEQ18 was medium polymorphic and the other 23 microsatellite loci were highly polymorphic.The average PIC(0.694 0),H(0.711 9)and E(2.9)of the eight donkey breed populations were all high,indicating that the gene polymorphisms and genetic diversity were relatively high.[Conclusion] The 24 microsatellite loci were effective markers for analysis of genetic relationships among the donkey breeds.The phylogenetic relationships among large and medium-sized donkey breeds were in accordance with their breeding history and distribution.展开更多
基金Supported by the Natural Science Foundation of Hebei Province(C2007000739)Doctoral Start Fund of Hebei Normal University of Science and Technology(2006D006)~~
文摘[Objective] The paper aimed to reveal the genetic diversity of large and medium-sized donkey breeds of China.[Method] The genetic polymorphisms of 24 microsatellites in the eight large and medium-sized donkey breeds were studied by means of PCR,polyacrylamide gel electrophoresis and silver staining.[Result] The microsatellite of NVHEQ18 was medium polymorphic and the other 23 microsatellite loci were highly polymorphic.The average PIC(0.694 0),H(0.711 9)and E(2.9)of the eight donkey breed populations were all high,indicating that the gene polymorphisms and genetic diversity were relatively high.[Conclusion] The 24 microsatellite loci were effective markers for analysis of genetic relationships among the donkey breeds.The phylogenetic relationships among large and medium-sized donkey breeds were in accordance with their breeding history and distribution.