Studies on plant peroxidases are reviewed in this paper, including enzyme types and localization, molecular structure, purification and identification and gene expression and regulation related to plant growth, develo...Studies on plant peroxidases are reviewed in this paper, including enzyme types and localization, molecular structure, purification and identification and gene expression and regulation related to plant growth, development, wounding and pathogen infection etc. The physiological functions of peroxida ses in plant are also discussed, which cover such as active oxygen metabolism, formation of lignin and suberin, degradation of auxin, oxidation of other compounds and responses for various environmental stresses, especially the pathogen attack.展开更多
文摘Studies on plant peroxidases are reviewed in this paper, including enzyme types and localization, molecular structure, purification and identification and gene expression and regulation related to plant growth, development, wounding and pathogen infection etc. The physiological functions of peroxida ses in plant are also discussed, which cover such as active oxygen metabolism, formation of lignin and suberin, degradation of auxin, oxidation of other compounds and responses for various environmental stresses, especially the pathogen attack.
文摘杨树受损伤后能诱导一些基因的表达,其编码的蛋白质可能在杨树的防卫反应中起一定作用。用PCR方法从新疆杨叶片中克隆出一个损伤诱导型Kun itz胰蛋白酶抑制剂基因PaTI1。序列分析表明:此基因不含内含子,其翻译起点上游具有‘TATA’和‘CCAAT’等转录控制元件,包含的阅读框架能编码一个长为213个氨基酸的多肽。此多肽与克隆自美洲山杨的PtTI2和PtTI1氨基酸序列同源性最高,分别为95%和80%,其N端存在一长度为27个氨基酸的信号肽。将此基因以融合蛋白的形式在大肠杆菌中进行表达,纯化后的融合蛋白对胰蛋白酶的活性有抑制作用,每8.5μg融合蛋白可完全抑制1μg牛胰蛋白酶的活性。W estern b lot分析表明融合蛋白与PtTI2特异的抗体之间有明显的血清学反应。
