目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑...目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。展开更多
The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-pea...The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-peak pattern. The first peak appeared at June and the second at September. The seasonal dynamic change of flavonoids in leafstalk, annual twig and perennial stem were different and inconspicuous. The flavonoid cumulated maximally in the leafblade and the followed was seccessively annual twig, leafstalk, and perennial stem. The flavonoid content in leafblade was 31.79 mg/g(DW), while that in annual twig, leafstalk and perennial stem was 5.41 mg/g(DW), 5.30 mg/g(DW) and 3.95 mg/g(DW) respectively. There was markedly higher flavonoid content in leafblade. It is considered that the leaf of Sargentodoxa cuneata is significant in further exploitation.展开更多
Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods us...Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods used in the ecological analysis of flavonids was that using ultraviolet and 70% ethanol was the best reagent for extraction. Heating was not necessary for extraction. The content of flavonoids from different organs was leafblade, annual twig, leafstalk and perennial stem in turn.展开更多
文摘目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。
文摘The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-peak pattern. The first peak appeared at June and the second at September. The seasonal dynamic change of flavonoids in leafstalk, annual twig and perennial stem were different and inconspicuous. The flavonoid cumulated maximally in the leafblade and the followed was seccessively annual twig, leafstalk, and perennial stem. The flavonoid content in leafblade was 31.79 mg/g(DW), while that in annual twig, leafstalk and perennial stem was 5.41 mg/g(DW), 5.30 mg/g(DW) and 3.95 mg/g(DW) respectively. There was markedly higher flavonoid content in leafblade. It is considered that the leaf of Sargentodoxa cuneata is significant in further exploitation.
文摘Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods used in the ecological analysis of flavonids was that using ultraviolet and 70% ethanol was the best reagent for extraction. Heating was not necessary for extraction. The content of flavonoids from different organs was leafblade, annual twig, leafstalk and perennial stem in turn.
