为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24...为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24 h Control组)和24 h试验组(24 h NMB组),每组10只,2个对照组和2个试验组分别按体重10μL/g腹腔注射生理盐水和10 nmol/L NMB溶液;称量注射前后小鼠体重,计算饲料用量,并观察小鼠行为特征;各组小鼠分别于注射后12小时、24小时摘眼球采血并处死取睾丸,通过ELISA法检测小鼠血清中睾酮的浓度;通过实时荧光定量PCR(q PCR)和蛋白印迹法(Western-blot)检测小鼠睾丸中NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA和蛋白表达量。结果表明:与12 h Control组相比,12 h NMB组小鼠行为无明显异常,体重增量、总摄食量略有减少,料重比略有增高,但差异不显著(P>0.05);NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与24 h Control组相比,24 h NMB组小鼠被毛粗乱、脾气暴躁,体重增量显著减少(P<0.05);总摄食量减少,料重比略有增高,但均差异不显著(P>0.05);NMB m RNA和蛋白表达量极显著降低(P<0.01),NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与12 h NMB组相比,24 h NMB组NMBR m RNA表达量极显著降低(P<0.01),24 h NMB组NMBR蛋白表达量显著增加(P<0.05)。说明小鼠腹腔注射NMB能够抑制小鼠摄食,但对小鼠睾丸中睾酮的合成和分泌影响不大。展开更多
随着“互联网+”的持续推进,由互联网技术和传统医药行业融合的医疗电商平台应运而生,为人们带来便利的同时,也面临着一系列挑战。因此,为了规范与完善医疗电商平台,保障人民权益,本文通过设计相关问卷收集数据对医疗电商平台需求及其...随着“互联网+”的持续推进,由互联网技术和传统医药行业融合的医疗电商平台应运而生,为人们带来便利的同时,也面临着一系列挑战。因此,为了规范与完善医疗电商平台,保障人民权益,本文通过设计相关问卷收集数据对医疗电商平台需求及其满意度的影响因素进行分析。根据描述性统计分析,用户对于医疗电商平台的需求大多在于药品的购买,对医疗电商平台附加服务的关注更倾向于运动、饮食方面的建议;利用SEM结构方程模型探究影响医疗电商平台用户购买满意度的因素,结果表明,医疗电商平台的网络安全性、网站设计特色、产品质量保证和购物便利性均对用户购物满意度有正面影响。最后根据本文所得结论,为政府管理部门和企业规范医疗电商平台市场、保障人民权益提供可行性的建议。With the continuous promotion of “Internet+”, medical e-commerce platforms have emerged as a result of the integration of Internet technology and traditional pharmaceutical industry, bringing great convenience to people’s lives while also facing a series of challenges. Therefore, in order to standardize and improve the medical e-commerce platform and protect people’s rights and interests, this paper analyzes the influencing factors of demand and satisfaction of the medical e-commerce platform by designing relevant questionnaires to collect data. According to the descriptive statistics analysis, users’ demand for medical e-commerce platforms mostly lies in the purchase of medicines, and the additional service concerns of medical e-commerce platforms are more inclined to exercise and dietary advice;SEM structural equation modeling is utilized to explore the factors affecting users’ satisfaction with medical e-commerce platforms’ purchases, and the results show that network security, website design features, product quality assurance and shopping convenience of medical e-commerce platforms have a positive impact on users’ shopping satisfaction. Finally, based on the conclusions obtained, feasible suggestions are provided for government management and enterprises to regulate the medical e-commerce platform market and protect people’s rights and interests.展开更多
文摘为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24 h Control组)和24 h试验组(24 h NMB组),每组10只,2个对照组和2个试验组分别按体重10μL/g腹腔注射生理盐水和10 nmol/L NMB溶液;称量注射前后小鼠体重,计算饲料用量,并观察小鼠行为特征;各组小鼠分别于注射后12小时、24小时摘眼球采血并处死取睾丸,通过ELISA法检测小鼠血清中睾酮的浓度;通过实时荧光定量PCR(q PCR)和蛋白印迹法(Western-blot)检测小鼠睾丸中NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA和蛋白表达量。结果表明:与12 h Control组相比,12 h NMB组小鼠行为无明显异常,体重增量、总摄食量略有减少,料重比略有增高,但差异不显著(P>0.05);NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与24 h Control组相比,24 h NMB组小鼠被毛粗乱、脾气暴躁,体重增量显著减少(P<0.05);总摄食量减少,料重比略有增高,但均差异不显著(P>0.05);NMB m RNA和蛋白表达量极显著降低(P<0.01),NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与12 h NMB组相比,24 h NMB组NMBR m RNA表达量极显著降低(P<0.01),24 h NMB组NMBR蛋白表达量显著增加(P<0.05)。说明小鼠腹腔注射NMB能够抑制小鼠摄食,但对小鼠睾丸中睾酮的合成和分泌影响不大。
文摘随着“互联网+”的持续推进,由互联网技术和传统医药行业融合的医疗电商平台应运而生,为人们带来便利的同时,也面临着一系列挑战。因此,为了规范与完善医疗电商平台,保障人民权益,本文通过设计相关问卷收集数据对医疗电商平台需求及其满意度的影响因素进行分析。根据描述性统计分析,用户对于医疗电商平台的需求大多在于药品的购买,对医疗电商平台附加服务的关注更倾向于运动、饮食方面的建议;利用SEM结构方程模型探究影响医疗电商平台用户购买满意度的因素,结果表明,医疗电商平台的网络安全性、网站设计特色、产品质量保证和购物便利性均对用户购物满意度有正面影响。最后根据本文所得结论,为政府管理部门和企业规范医疗电商平台市场、保障人民权益提供可行性的建议。With the continuous promotion of “Internet+”, medical e-commerce platforms have emerged as a result of the integration of Internet technology and traditional pharmaceutical industry, bringing great convenience to people’s lives while also facing a series of challenges. Therefore, in order to standardize and improve the medical e-commerce platform and protect people’s rights and interests, this paper analyzes the influencing factors of demand and satisfaction of the medical e-commerce platform by designing relevant questionnaires to collect data. According to the descriptive statistics analysis, users’ demand for medical e-commerce platforms mostly lies in the purchase of medicines, and the additional service concerns of medical e-commerce platforms are more inclined to exercise and dietary advice;SEM structural equation modeling is utilized to explore the factors affecting users’ satisfaction with medical e-commerce platforms’ purchases, and the results show that network security, website design features, product quality assurance and shopping convenience of medical e-commerce platforms have a positive impact on users’ shopping satisfaction. Finally, based on the conclusions obtained, feasible suggestions are provided for government management and enterprises to regulate the medical e-commerce platform market and protect people’s rights and interests.
