利用 RAPD随机引物和斑茅 5 S r RNA间隔序列 (ITS)引物对斑茅与甘蔗的杂种 F1 、 F2 代进行了分子鉴定 ,获得了与含有斑茅血缘有关的 RAPD分子标记 3个及 5 S r RNA间隔序列标记 1个 ;其中 ,5 S r RNA间隔序列标记可用于斑茅杂种后代碱...利用 RAPD随机引物和斑茅 5 S r RNA间隔序列 (ITS)引物对斑茅与甘蔗的杂种 F1 、 F2 代进行了分子鉴定 ,获得了与含有斑茅血缘有关的 RAPD分子标记 3个及 5 S r RNA间隔序列标记 1个 ;其中 ,5 S r RNA间隔序列标记可用于斑茅杂种后代碱法 DNA模板的快速检测。展开更多
[ Objective] The study aimed to reveal the biological nitrogen fixation capacity by sugarcane from Brazil under the ecological conditions of Guangxi, and to provide reference for study on the biological nitrogen fixat...[ Objective] The study aimed to reveal the biological nitrogen fixation capacity by sugarcane from Brazil under the ecological conditions of Guangxi, and to provide reference for study on the biological nitrogen fixation capacity by sugarcane and related generalization and application. [ Method] The ^15N isotopic fertilizer was solely applied on plants of three sugarcane cultivars planted in greenhouse with no other fertilizer forms applied, meanwhile virus-free stem seedling was regarded as control, to measure their biological nitrogen fixation capacity using ^15N isotope. [ Result ] The nitrogen fixation rate of B8 from Brazil reached 26.91%, while Guitang 11 and RIC16 presented no or poor nitrogen fixation capacity. [ Conclusion] The sugarcane eultivar B8 from Brazil showed some nitrogen fixation capacity under the ecological conditions of Guangxi.展开更多
[Objective]The aim was to understand the change characteristics of sugarcane traits and evaluate the cold tolerance of sugarcane varieties under the drought and frost conditions.[Method] The experiment was carried out...[Objective]The aim was to understand the change characteristics of sugarcane traits and evaluate the cold tolerance of sugarcane varieties under the drought and frost conditions.[Method] The experiment was carried out in Ziyuan County,Guangxi Province where the frost occurred often with 21 domestic and abroad sugarcane varieties(elites).[Result] There were significant changes in the brix,green leaf number and photosynthetic rate of sugarcane before and after light frost and decreased more in cold-sensitive varieties.However,the correlation was only significant between the damage rate of stem length and internode,percentage of green leaves after heavy frost in relation to sugarcane brix and brix changes after light frost.Further analysis showed that the evaluation for cold tolerance of sugarcane would be more simple and reliable with traits of the damage rate of stem length and percentage of upper green leaves.The evaluation for varieties indicated that if it was not carried out for cold-tolerance identification in breeding program,the percentage of sugarcane varieties with good cold-tolerance will be lower than30%,and higher than 60% with poor cold-tolerance in subtropical and tropical regions.[Conclusion] This study had provided theoretical basis for the cold-resistant evaluation of sugar cane and the breeding of the varieties of cold-resistant.展开更多
文摘利用 RAPD随机引物和斑茅 5 S r RNA间隔序列 (ITS)引物对斑茅与甘蔗的杂种 F1 、 F2 代进行了分子鉴定 ,获得了与含有斑茅血缘有关的 RAPD分子标记 3个及 5 S r RNA间隔序列标记 1个 ;其中 ,5 S r RNA间隔序列标记可用于斑茅杂种后代碱法 DNA模板的快速检测。
基金National Natural Science Foundation of China (3026005430660085)+1 种基金Key Project of Guangxi Academy of Agricultural Sciences (2004002)Natural Science Foundation in Guangxi Zhuang Autonomous Region (0639011)~~
文摘[ Objective] The study aimed to reveal the biological nitrogen fixation capacity by sugarcane from Brazil under the ecological conditions of Guangxi, and to provide reference for study on the biological nitrogen fixation capacity by sugarcane and related generalization and application. [ Method] The ^15N isotopic fertilizer was solely applied on plants of three sugarcane cultivars planted in greenhouse with no other fertilizer forms applied, meanwhile virus-free stem seedling was regarded as control, to measure their biological nitrogen fixation capacity using ^15N isotope. [ Result ] The nitrogen fixation rate of B8 from Brazil reached 26.91%, while Guitang 11 and RIC16 presented no or poor nitrogen fixation capacity. [ Conclusion] The sugarcane eultivar B8 from Brazil showed some nitrogen fixation capacity under the ecological conditions of Guangxi.
基金Supported by National Science and Technology Support Program(2008BADB8B01,2007BAD30B02,2007BAD30B05)Modern Agricultural Technology System Special Fund Project(nycytx-024-01-03)Guangxi Scientific and Technological Project(0782004-2,0782004-5)~~
文摘[Objective]The aim was to understand the change characteristics of sugarcane traits and evaluate the cold tolerance of sugarcane varieties under the drought and frost conditions.[Method] The experiment was carried out in Ziyuan County,Guangxi Province where the frost occurred often with 21 domestic and abroad sugarcane varieties(elites).[Result] There were significant changes in the brix,green leaf number and photosynthetic rate of sugarcane before and after light frost and decreased more in cold-sensitive varieties.However,the correlation was only significant between the damage rate of stem length and internode,percentage of green leaves after heavy frost in relation to sugarcane brix and brix changes after light frost.Further analysis showed that the evaluation for cold tolerance of sugarcane would be more simple and reliable with traits of the damage rate of stem length and percentage of upper green leaves.The evaluation for varieties indicated that if it was not carried out for cold-tolerance identification in breeding program,the percentage of sugarcane varieties with good cold-tolerance will be lower than30%,and higher than 60% with poor cold-tolerance in subtropical and tropical regions.[Conclusion] This study had provided theoretical basis for the cold-resistant evaluation of sugar cane and the breeding of the varieties of cold-resistant.