The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O...The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.展开更多
Fresh potato (Solanum tuberosum L.) tuber slices had no capacity of the alternative respiration pathway (ARP). But this capacity could be induced drastically by a 24 h aging process. Western blotting results with a mo...Fresh potato (Solanum tuberosum L.) tuber slices had no capacity of the alternative respiration pathway (ARP). But this capacity could be induced drastically by a 24 h aging process. Western blotting results with a monoclonal antibody against the alternative oxidase (AOX) showed no evidence of AOX in fresh slices. However, an AOX protein of 36 kD was clearly detected in slices aged for 12 or 24 h(Fig.1). Under treatments with ACC and with CoCl 2, the ARP capacity of aged potato slices was enhanced and reduced, respectively. Correspondingly, the extent of AOX protein’s expression was enhanced by ACC and reduced by CoCl 2. The above results give evidence that the regulatory effect of endogenous ethylene on the development of ARP capacity in aging potato tuber slices is exerted through the induction of the expression of AOX therein.展开更多
基金国家质量监督检验检疫总局科技计划项目"基于Pyro Mark ID平台的马铃薯Y病毒检疫鉴定技术研究"(2008IK242)国家质量监督检验检疫总局科技计划项目"环介导等温扩增技术(LAMP)检测马铃薯病毒方法的研究"(2013IK274)+1 种基金国家标准委国家标准计划项目"马铃薯Y病毒检疫鉴定方法"(20120579-T-469)国家自然科学基金资助项目"交替氧化酶诱导植物抗病毒侵染的机理研究"(30870187)
文摘The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.
文摘Fresh potato (Solanum tuberosum L.) tuber slices had no capacity of the alternative respiration pathway (ARP). But this capacity could be induced drastically by a 24 h aging process. Western blotting results with a monoclonal antibody against the alternative oxidase (AOX) showed no evidence of AOX in fresh slices. However, an AOX protein of 36 kD was clearly detected in slices aged for 12 or 24 h(Fig.1). Under treatments with ACC and with CoCl 2, the ARP capacity of aged potato slices was enhanced and reduced, respectively. Correspondingly, the extent of AOX protein’s expression was enhanced by ACC and reduced by CoCl 2. The above results give evidence that the regulatory effect of endogenous ethylene on the development of ARP capacity in aging potato tuber slices is exerted through the induction of the expression of AOX therein.
