Objective:To investigate the alteration of delayed memory and its relationship with neurogenesis and angiogenesis in vascular dementia(VD)rats after moxibustion therapy.Methods:Two hundred adult male SPF Wistar rats w...Objective:To investigate the alteration of delayed memory and its relationship with neurogenesis and angiogenesis in vascular dementia(VD)rats after moxibustion therapy.Methods:Two hundred adult male SPF Wistar rats were chosen for the experiment.Thirty-six rats were randomly selected as the sham operation group.Except for rats in the sham operation group(n=36),the others were made into VD models by bilateral comm on carotid arteries occlusi on(BCCAo).After modeli ng,the 108 survived rats were ran domly divided into 3 groups:a model group,a neural stem cells(NSCs)plus endothelial progenitor cells(EPCs)moxibustion group and a NSCs moxibustion group.Co-transplanted implant was transplanted into the rats in the NSCs plus EPCs moxibustion group,and the rats in the NSCs moxibustion group were transplanted by NSCs only.The NSCs plus EPCs moxibustion group and the NSCs moxibustion group received suspended moxibustion therapy at Baihui(GV 20),Dazhui(GV 14)and Shenting(GV 24),(each group was divided into 3 subgroups by the treatment course as 1,2 and 3 courses).Every group was measured by Morris water maze to evaluate its delayed memory after 3 treatment courses and the rat's brain was taken out after perfusion of 4%paraformaldehyde one day after 1,2 and 3 treatment courses,respectively.Marker protein expression was detected by laser con focal microscope to an a lyze the effect on neuroge nesis and an gioge nesis.Results:VD rats showed delayed memory in Morris water maze test 3 d after ischemic injury.After 3 courses of moxibustion therapy,VD-induced delayed memory deficits were improved in the NSCs plus EPCs moxibustion group and the NSCs moxibustion group.The expressions of nestin,doublecortin(DCX)and CD34 increased significantly in the two moxibusiton groups after every treatment course(all P<0.05),which might contribute to the neurogenesis and angiogenesis in hippocampus.In addition,compared with the rats in the NSCs moxibustion group,the expressions of nestin,DCX and CD34 increased significantly in the NSCs plus EPCs moxibustion group(P<0.05).Conclusion:Moxibustion can reverse VD-induced delayed memory deficits,which may be related to the promotion of neurogenesis and angiogenesis.展开更多
Objective:To observe the influence of moxibustion on learning and memory ability in the rats with vascular dementia(VD) and explore the potential effect mechanism.Methods:A total of 80 rats,screened by Morris water ma...Objective:To observe the influence of moxibustion on learning and memory ability in the rats with vascular dementia(VD) and explore the potential effect mechanism.Methods:A total of 80 rats,screened by Morris water maze,were randomly divided into a shamoperation group,a model group,a neural stem cells(NSCs) group,a NSCs+piracetam group and a NSCs+moxibustion group,16 rats in each group.After corresponding treatments,Morris water maze and immunofluorescence technique were adopted to evaluate the therapeutic effect respectively.Results:Comparison among groups after modeling:compared with the sham-operation group,the escape latency was longer(P<0.01) and the times of crossing platform were reduced(P<0.01) in the rats of the model group.Comparison among groups after treatment:compared with the model group,the escape latency was shortened(P<0.01) and the times of crossing platform were increased(P<0.05) in the rats of the NSCs group.Compared with the NSCs group,the escape latency was shorter and the expressions of the hippocampus NR2B/EGFP and PKM ξ/EGFP expression level increased(all P<0.05) in the rats of the NSCs+piracetam group and the NSCs+moxibustion group(all P <0.05).Compared with the NSCs+piracetam group,the escape latency was shorter and the expressions of the hippocampus NR2B/EGFP and PKM ξ/EGFP expression level were higher in the rats of the moxibustion+NSCs group(all P<0.05).Conclusion:Moxibustion improves the spatial learning and memory ability of the VD rats and promotes the reconstruction of neurogenesis and synaptic function,which may be related to the up-regulation of the expressions of hippocampus NR2B and PKMξ expressions.展开更多
文摘Objective:To investigate the alteration of delayed memory and its relationship with neurogenesis and angiogenesis in vascular dementia(VD)rats after moxibustion therapy.Methods:Two hundred adult male SPF Wistar rats were chosen for the experiment.Thirty-six rats were randomly selected as the sham operation group.Except for rats in the sham operation group(n=36),the others were made into VD models by bilateral comm on carotid arteries occlusi on(BCCAo).After modeli ng,the 108 survived rats were ran domly divided into 3 groups:a model group,a neural stem cells(NSCs)plus endothelial progenitor cells(EPCs)moxibustion group and a NSCs moxibustion group.Co-transplanted implant was transplanted into the rats in the NSCs plus EPCs moxibustion group,and the rats in the NSCs moxibustion group were transplanted by NSCs only.The NSCs plus EPCs moxibustion group and the NSCs moxibustion group received suspended moxibustion therapy at Baihui(GV 20),Dazhui(GV 14)and Shenting(GV 24),(each group was divided into 3 subgroups by the treatment course as 1,2 and 3 courses).Every group was measured by Morris water maze to evaluate its delayed memory after 3 treatment courses and the rat's brain was taken out after perfusion of 4%paraformaldehyde one day after 1,2 and 3 treatment courses,respectively.Marker protein expression was detected by laser con focal microscope to an a lyze the effect on neuroge nesis and an gioge nesis.Results:VD rats showed delayed memory in Morris water maze test 3 d after ischemic injury.After 3 courses of moxibustion therapy,VD-induced delayed memory deficits were improved in the NSCs plus EPCs moxibustion group and the NSCs moxibustion group.The expressions of nestin,doublecortin(DCX)and CD34 increased significantly in the two moxibusiton groups after every treatment course(all P<0.05),which might contribute to the neurogenesis and angiogenesis in hippocampus.In addition,compared with the rats in the NSCs moxibustion group,the expressions of nestin,DCX and CD34 increased significantly in the NSCs plus EPCs moxibustion group(P<0.05).Conclusion:Moxibustion can reverse VD-induced delayed memory deficits,which may be related to the promotion of neurogenesis and angiogenesis.
基金Supported by National Natural Science Foundation of China:81873370。
文摘Objective:To observe the influence of moxibustion on learning and memory ability in the rats with vascular dementia(VD) and explore the potential effect mechanism.Methods:A total of 80 rats,screened by Morris water maze,were randomly divided into a shamoperation group,a model group,a neural stem cells(NSCs) group,a NSCs+piracetam group and a NSCs+moxibustion group,16 rats in each group.After corresponding treatments,Morris water maze and immunofluorescence technique were adopted to evaluate the therapeutic effect respectively.Results:Comparison among groups after modeling:compared with the sham-operation group,the escape latency was longer(P<0.01) and the times of crossing platform were reduced(P<0.01) in the rats of the model group.Comparison among groups after treatment:compared with the model group,the escape latency was shortened(P<0.01) and the times of crossing platform were increased(P<0.05) in the rats of the NSCs group.Compared with the NSCs group,the escape latency was shorter and the expressions of the hippocampus NR2B/EGFP and PKM ξ/EGFP expression level increased(all P<0.05) in the rats of the NSCs+piracetam group and the NSCs+moxibustion group(all P <0.05).Compared with the NSCs+piracetam group,the escape latency was shorter and the expressions of the hippocampus NR2B/EGFP and PKM ξ/EGFP expression level were higher in the rats of the moxibustion+NSCs group(all P<0.05).Conclusion:Moxibustion improves the spatial learning and memory ability of the VD rats and promotes the reconstruction of neurogenesis and synaptic function,which may be related to the up-regulation of the expressions of hippocampus NR2B and PKMξ expressions.
