目的探究阿司匹林通过调节铁死亡对氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation,OGD/R)诱导的小鼠神经元HT22细胞损伤的作用。方法体外培养小鼠海马神经元HT22细胞,选取HT22细胞分为对照组、模型组、低剂量组、中剂量组、...目的探究阿司匹林通过调节铁死亡对氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation,OGD/R)诱导的小鼠神经元HT22细胞损伤的作用。方法体外培养小鼠海马神经元HT22细胞,选取HT22细胞分为对照组、模型组、低剂量组、中剂量组、高剂量组(n=3),除对照组外,其余4组建立OGD/R神经元细胞损伤模型,低、中、高剂量组分别给予阿司匹林100、200、400μg/ml处理。检测各组细胞活力及炎性因子肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)、白细胞介素(interleukin,IL)1β、IL-6水平;试剂盒检测超氧化物歧化酶、过氧化氢酶、谷胱甘肽、活性氧、乳酸脱氢酶、Fe^(2+)、丙二醛水平;Western blot检测铁死亡相关蛋白溶质载体家族7成员11(solute carrier family 7 members 11,SLC7A11)、谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)以及酰基辅酶A合成酶长链家族成员4(acyl-coa synthase long chain family member 4,ACSL4)水平。结果模型组细胞活力明显低于对照组,差异有统计学意义(0.49±0.07 vs 1.00±0.12,P<0.01),低、中、高剂量组细胞活力明显高于模型组,差异有统计学意义(0.72±0.10 vs 0.49±0.07,P<0.05;0.87±0.10 vs 0.49±0.07,P<0.01;0.93±0.07 vs 0.49±0.07,P<0.01)。与对照组比较,模型组TNF-α、IL-1β、IL-6、活性氧、乳酸脱氢酶、Fe^(2+)、丙二醛、ACSL4蛋白表达明显升高,超氧化物歧化酶、抗氧化酶、谷胱甘肽、SLC7A11、GPX4蛋白表达明显降低,差异有统计学意义(P<0.01)。与模型组比较,低、中、高剂量组TNF-α、IL-1β、IL-6、活性氧、乳酸脱氢酶Fe^(2+)、丙二醛、ACSL4蛋白表达明显降低,超氧化物歧化酶、抗氧化酶、谷胱甘肽、SLC7A11、GPX4蛋白表达明显升高,差异有统计学意义(P<0.05,P<0.01)。结论阿司匹林可以通过调节铁死亡,减轻OGD/R诱导的小鼠神经元HT22细胞损伤,且呈剂量依赖性。展开更多
农村电商是乡村产业振兴的活力源泉,乡村产业振兴为农村电商发展提供有利条件,二者相辅相成、相互促进。电子商务作为一种新型的商业形态走向农村,在助推乡村产业振兴的同时,面临着基础设施建设滞后、专业电商人才队伍空缺、农产品品牌...农村电商是乡村产业振兴的活力源泉,乡村产业振兴为农村电商发展提供有利条件,二者相辅相成、相互促进。电子商务作为一种新型的商业形态走向农村,在助推乡村产业振兴的同时,面临着基础设施建设滞后、专业电商人才队伍空缺、农产品品牌效应薄弱等问题。本文针对这些存在的现实困境问题,提出了农村电商赋能乡村产业振兴的三条实践路径,即强化农村电商基础设施建设、培育和引进农村电商人才以及打造农产品电商品牌。Rural e-commerce is the source of vitality for the revitalization of rural industry, and rural industry revitalization provides favorable conditions for the development of rural e-commerce, and the two complement each other and promote each other. As a new form of commerce, e-commerce is moving to rural areas. While promoting the revitalization of rural industries, it faces some problems such as lagging infrastructure construction, lack of professional e-commerce talent team, and weak brand effect of agricultural products. In view of these realistic difficulties, this paper puts forward three practical paths for rural e-commerce to empower rural industry revitalization, namely, strengthening rural e-commerce infrastructure construction, cultivating and introducing rural e-commerce talents, and building agricultural products e-commerce brand.展开更多
文摘目的探究阿司匹林通过调节铁死亡对氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation,OGD/R)诱导的小鼠神经元HT22细胞损伤的作用。方法体外培养小鼠海马神经元HT22细胞,选取HT22细胞分为对照组、模型组、低剂量组、中剂量组、高剂量组(n=3),除对照组外,其余4组建立OGD/R神经元细胞损伤模型,低、中、高剂量组分别给予阿司匹林100、200、400μg/ml处理。检测各组细胞活力及炎性因子肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)、白细胞介素(interleukin,IL)1β、IL-6水平;试剂盒检测超氧化物歧化酶、过氧化氢酶、谷胱甘肽、活性氧、乳酸脱氢酶、Fe^(2+)、丙二醛水平;Western blot检测铁死亡相关蛋白溶质载体家族7成员11(solute carrier family 7 members 11,SLC7A11)、谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)以及酰基辅酶A合成酶长链家族成员4(acyl-coa synthase long chain family member 4,ACSL4)水平。结果模型组细胞活力明显低于对照组,差异有统计学意义(0.49±0.07 vs 1.00±0.12,P<0.01),低、中、高剂量组细胞活力明显高于模型组,差异有统计学意义(0.72±0.10 vs 0.49±0.07,P<0.05;0.87±0.10 vs 0.49±0.07,P<0.01;0.93±0.07 vs 0.49±0.07,P<0.01)。与对照组比较,模型组TNF-α、IL-1β、IL-6、活性氧、乳酸脱氢酶、Fe^(2+)、丙二醛、ACSL4蛋白表达明显升高,超氧化物歧化酶、抗氧化酶、谷胱甘肽、SLC7A11、GPX4蛋白表达明显降低,差异有统计学意义(P<0.01)。与模型组比较,低、中、高剂量组TNF-α、IL-1β、IL-6、活性氧、乳酸脱氢酶Fe^(2+)、丙二醛、ACSL4蛋白表达明显降低,超氧化物歧化酶、抗氧化酶、谷胱甘肽、SLC7A11、GPX4蛋白表达明显升高,差异有统计学意义(P<0.05,P<0.01)。结论阿司匹林可以通过调节铁死亡,减轻OGD/R诱导的小鼠神经元HT22细胞损伤,且呈剂量依赖性。
文摘农村电商是乡村产业振兴的活力源泉,乡村产业振兴为农村电商发展提供有利条件,二者相辅相成、相互促进。电子商务作为一种新型的商业形态走向农村,在助推乡村产业振兴的同时,面临着基础设施建设滞后、专业电商人才队伍空缺、农产品品牌效应薄弱等问题。本文针对这些存在的现实困境问题,提出了农村电商赋能乡村产业振兴的三条实践路径,即强化农村电商基础设施建设、培育和引进农村电商人才以及打造农产品电商品牌。Rural e-commerce is the source of vitality for the revitalization of rural industry, and rural industry revitalization provides favorable conditions for the development of rural e-commerce, and the two complement each other and promote each other. As a new form of commerce, e-commerce is moving to rural areas. While promoting the revitalization of rural industries, it faces some problems such as lagging infrastructure construction, lack of professional e-commerce talent team, and weak brand effect of agricultural products. In view of these realistic difficulties, this paper puts forward three practical paths for rural e-commerce to empower rural industry revitalization, namely, strengthening rural e-commerce infrastructure construction, cultivating and introducing rural e-commerce talents, and building agricultural products e-commerce brand.
