目的探索高危型人乳头瘤状病毒(high-risk human papillomavirus,HR-HPV)感染阴性与阳性女性之间阴道菌群构成的差异。方法采集2014年9月至2015年9月间于我科就诊的33例未感染HR-HPV(HPV^-)女性及98例感染HR-HPV(HPV^+)患者的阴道分泌物...目的探索高危型人乳头瘤状病毒(high-risk human papillomavirus,HR-HPV)感染阴性与阳性女性之间阴道菌群构成的差异。方法采集2014年9月至2015年9月间于我科就诊的33例未感染HR-HPV(HPV^-)女性及98例感染HR-HPV(HPV^+)患者的阴道分泌物,分别对每个样本进行细菌总基因组DNA提取、16S rRNA V4区基因扩增及采用Illumina高通量测序技术对扩增的PCR产物进行测序等步骤,然后通过BIPES、TSC、GAST等程序,分析阴道菌群物种丰度和结构,并对两组女性的阴道菌群构成进行比较。结果 HPV^-组女性阴道菌群以乳酸杆菌为优势菌属,占81.54%,同时含有少量的加德纳菌属、链球菌属、普氏菌属等。HPV^+组患者阴道菌群中乳酸杆菌属则显著减少(40.48%),加德纳菌属显著增加(32.35%),同时普氏菌属、奇异菌属、厌氧球菌属等多种厌氧菌属相对丰度也有所增加。在HPV^+组女性的阴道菌群中发现布鲁菌属的存在,而HPV^-组中未见此菌属。结论阴道菌群失调与HR-HPV感染之间存在相关性,HR-HPV感染者阴道菌群中乳酸杆菌属显著减少,加德纳菌属等多种厌氧菌属过度增殖,并发现有布鲁菌属存在。展开更多
The retrieval and management of components play important roles on the Component Based Software De-velopment (CBSD), which is an effective way to support software reuse. Reusable component library is one of thefoundat...The retrieval and management of components play important roles on the Component Based Software De-velopment (CBSD), which is an effective way to support software reuse. Reusable component library is one of thefoundation establishments in it. It is well able to provide component representation, classification, certification, stor-age, retrieval, evaluation and feedback, interoperable libraries, etc. This paper is a summarization on the technolo-gies in component library. It introduces their current status, presents four component libraries, and outlines the fu-ture trends.展开更多
文摘The retrieval and management of components play important roles on the Component Based Software De-velopment (CBSD), which is an effective way to support software reuse. Reusable component library is one of thefoundation establishments in it. It is well able to provide component representation, classification, certification, stor-age, retrieval, evaluation and feedback, interoperable libraries, etc. This paper is a summarization on the technolo-gies in component library. It introduces their current status, presents four component libraries, and outlines the fu-ture trends.