目的:通过基因表达汇编(Gene Expression Omnibus,GEO)公共数据库,探讨6-磷酸果糖激酶2(6-phosphofructo-2-kinase,PFKFB3)在肝细胞癌中的表达情况,并进一步探索其与肝细胞癌临床病理特征的关系,评价PFKFB3对肝细胞癌术后患者预后评价...目的:通过基因表达汇编(Gene Expression Omnibus,GEO)公共数据库,探讨6-磷酸果糖激酶2(6-phosphofructo-2-kinase,PFKFB3)在肝细胞癌中的表达情况,并进一步探索其与肝细胞癌临床病理特征的关系,评价PFKFB3对肝细胞癌术后患者预后评价的意义,预测PFKFB3推动肝细胞癌发展的机制.方法:检索、下载美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)的肿瘤公共数据集,对表达谱资料及临床信息进行分析;利用基因集富集分析(gene set enrichment analysis,GSEA)方法,分析受PFKFB3调控的相关基因.结果:PFKFB3在高T分期肿瘤中高表达(P<0.05);在不同年龄、谷丙转氨酶(alaninet r a n s a m i n a s e,A LT)、肿瘤大小、肿瘤结节数目、T分期、肝细胞癌巴塞罗那分期(Barcelona Clinic Liver Cancer stage,BCLCstage)、意大利肝细胞癌项目分期(CancerLiver Italian Program stage,CLIP stage)的肝细胞癌患者中,PFKFB3的表达均有显著性差异(P<0.05);PFKFB3的表达状态与肝细胞癌患者术后的总体生存和复发相关(P<0.05);PFKFB3高表达样本富集了与细胞增殖、抗凋亡、代谢相关的基因集.结论:PFKFB3与肝细胞癌多个病理指标相关,且可以做为潜在的判断肝细胞癌患者预后的标志物和治疗肿瘤的靶标.展开更多
The impact of prior cesarean section（CS） on the pregnancy and neonatal outcomes of in vitro fertilization and embryo transfer（IVF-ET） was investigated. A retrospective analysis was performed on 144 patients with p...The impact of prior cesarean section（CS） on the pregnancy and neonatal outcomes of in vitro fertilization and embryo transfer（IVF-ET） was investigated. A retrospective analysis was performed on 144 patients with prior CS between January 2013 and December 2015. The pregnancy, delivery, and neonatal outcomes of patients who had previous CS delivery and received IVF-ET were analyzed. The control group comprised 166 patients who had only previous vaginal delivery（VD） and received IVF-ET during the same period. The results showed that the basal follicle stimulating hormone level, estradiol level on human chorionic gonadotropin（h CG） day, gonadotrophin dosage, duration of stimulation, retrieved oocytes, fertilization rate, high-quality embryo rate, multiple birth rate, abortion rate and ectopic pregnancy rate had no significant difference between the two groups（P〉0.05）. The pregnancy rate（40.28% vs. 54.22%） and implantation rate（24.01% vs. 34.67%） were significantly lower（P〈0.05）, and the ratio of embryo difficulty transfer（9/144 vs. 0/166） was significantly higher in CS group than in VD group. The risk of pernicious placenta previa and postpartum hemorrhage in twin deliveries was significantly increased in CS group as compared with that in VD group（P〈0.05）, and gestational age and neonatal birth weight were significantly reduced in twin deliveries as compared with singleton deliveries in both groups（P〈0.05）. It was suggested that the existence of CS scar may impact embryo implantation and clinical pregnancy outcome, and increase the difficulty of ET. We should limit the number of transfer embryos to avoid multiple pregnancies and strengthen gestational supervision in patients with cesarean scar.展开更多
文摘目的:通过基因表达汇编(Gene Expression Omnibus,GEO)公共数据库,探讨6-磷酸果糖激酶2(6-phosphofructo-2-kinase,PFKFB3)在肝细胞癌中的表达情况,并进一步探索其与肝细胞癌临床病理特征的关系,评价PFKFB3对肝细胞癌术后患者预后评价的意义,预测PFKFB3推动肝细胞癌发展的机制.方法:检索、下载美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)的肿瘤公共数据集,对表达谱资料及临床信息进行分析;利用基因集富集分析(gene set enrichment analysis,GSEA)方法,分析受PFKFB3调控的相关基因.结果:PFKFB3在高T分期肿瘤中高表达(P<0.05);在不同年龄、谷丙转氨酶(alaninet r a n s a m i n a s e,A LT)、肿瘤大小、肿瘤结节数目、T分期、肝细胞癌巴塞罗那分期(Barcelona Clinic Liver Cancer stage,BCLCstage)、意大利肝细胞癌项目分期(CancerLiver Italian Program stage,CLIP stage)的肝细胞癌患者中,PFKFB3的表达均有显著性差异(P<0.05);PFKFB3的表达状态与肝细胞癌患者术后的总体生存和复发相关(P<0.05);PFKFB3高表达样本富集了与细胞增殖、抗凋亡、代谢相关的基因集.结论:PFKFB3与肝细胞癌多个病理指标相关,且可以做为潜在的判断肝细胞癌患者预后的标志物和治疗肿瘤的靶标.
文摘The impact of prior cesarean section（CS） on the pregnancy and neonatal outcomes of in vitro fertilization and embryo transfer（IVF-ET） was investigated. A retrospective analysis was performed on 144 patients with prior CS between January 2013 and December 2015. The pregnancy, delivery, and neonatal outcomes of patients who had previous CS delivery and received IVF-ET were analyzed. The control group comprised 166 patients who had only previous vaginal delivery（VD） and received IVF-ET during the same period. The results showed that the basal follicle stimulating hormone level, estradiol level on human chorionic gonadotropin（h CG） day, gonadotrophin dosage, duration of stimulation, retrieved oocytes, fertilization rate, high-quality embryo rate, multiple birth rate, abortion rate and ectopic pregnancy rate had no significant difference between the two groups（P〉0.05）. The pregnancy rate（40.28% vs. 54.22%） and implantation rate（24.01% vs. 34.67%） were significantly lower（P〈0.05）, and the ratio of embryo difficulty transfer（9/144 vs. 0/166） was significantly higher in CS group than in VD group. The risk of pernicious placenta previa and postpartum hemorrhage in twin deliveries was significantly increased in CS group as compared with that in VD group（P〈0.05）, and gestational age and neonatal birth weight were significantly reduced in twin deliveries as compared with singleton deliveries in both groups（P〈0.05）. It was suggested that the existence of CS scar may impact embryo implantation and clinical pregnancy outcome, and increase the difficulty of ET. We should limit the number of transfer embryos to avoid multiple pregnancies and strengthen gestational supervision in patients with cesarean scar.
