随着我国乡村振兴战略的实施,农村青少年科普教育的重要性日益凸显。本文从农村青少年科普教育的现状分析入手,探讨了在乡村振兴战略下,如何加强农村青少年科普教育的路径。一是优化农村科普教育环境,创新科普教育模式和方法,加大对农...随着我国乡村振兴战略的实施,农村青少年科普教育的重要性日益凸显。本文从农村青少年科普教育的现状分析入手,探讨了在乡村振兴战略下,如何加强农村青少年科普教育的路径。一是优化农村科普教育环境,创新科普教育模式和方法,加大对农村青少年科普教育的投入,利用现代信息技术,打破地域限制,营造农村科普教育氛围。二是加强农村教师队伍建设和培训,充分调动农村教师队伍的积极性和主动性,提高农村教师整体素质。三是建立全方位的支持体系,出台相关政策,为农村青少年提供更多优质科普资源。本文旨在为乡村振兴战略下加强农村科普教育提供理论指导和实践参考。With the implementation of China’s rural revitalization strategy, the importance of popular science education for rural teenagers has become increasingly prominent. Based on the analysis of the current situation of popular science education for rural teenagers, this paper discusses how to strengthen the popular science education for rural teenagers under the strategy of rural revitalization. The first step is to optimize the environment of rural popular science education, innovate the modes and methods of popular science education, increase investment in rural teenager popular science education, use modern information technology, break geographical restrictions, and create an atmosphere of rural popular science education. The second is to strengthen the construction and training of rural teachers, fully mobilize the enthusiasm and initiative of rural teachers and improve the overall quality of rural teachers. The third is to establish an all-round support system and introduce relevant policies to provide more high-quality popular science resources for rural teenagers. This paper aims to provide theoretical guidance and practical reference for strengthening rural popular science education under the strategy of rural revitalization.展开更多
目的观察磷脂酰肌醇-3激酶(PI3K)-蛋白质丝氨酸/苏氨酸激酶(AKt)-内皮型一氧化氮合酶(e NOS)信号转导通路在硫化氢(H2S)抑制内皮素-1(endothelin-1,ET-1)诱导心肌肥大过程中的作用。方法体外培养原代心肌细胞,将其随机分为6组,每组4孔,...目的观察磷脂酰肌醇-3激酶(PI3K)-蛋白质丝氨酸/苏氨酸激酶(AKt)-内皮型一氧化氮合酶(e NOS)信号转导通路在硫化氢(H2S)抑制内皮素-1(endothelin-1,ET-1)诱导心肌肥大过程中的作用。方法体外培养原代心肌细胞,将其随机分为6组,每组4孔,1对照组:加入等体积无血清的DMEM培养基;2肥大(ET-1)组:加入终浓度为10-8 mol/L的ET-1;剩余4组为实验组,各组分别加入不同终浓度的H2S供体-Na HS:310-15 M Na HS组:加入10-15 mol/L Na HS+10-8 mol/l ET-1;410-14 M Na HS组:加入10-14 mol/L Na HS+10-8 mol/L ET-1;510-13 M Na HS组:加入10-13 mol/L Na HS+10-8 mol/L ET-1;610-12 M Na HS组:加入10-12 mol/L Na HS+10-8 mol/L ET-1。上述各组药物分别刺激24 h后测定心肌细胞表面积、细胞总蛋白含量、培养液NO含量,RT-PCR检测心肌细胞心房利钠肽(atrial natriuretic peptide,ANP)、脑钠肽(B-type natriuretic peptide,BNP)、磷脂酰肌醇-3激酶(phosphatidylinositol-3-kinase,PI3K)、蛋白激酶B(protein kinase B,PKB/AKt)、e NOS m RNA水平,Western Blot技术检测总AKt和磷酸化AKt蛋白表达含量。结果肥大(ET-1)组的心肌细胞表面积(1933.80±143.06)和细胞总蛋白含量(367.51±25.9)均高于对照组(787.27±107.66,218.55±21.28,P<0.05),ANP及BNP m RNA的表达量也明显增加(P<0.05),但PI3K、AKt、e NOS m RNA表达水平,磷酸化AKt程度和NO的释放量(4.60±0.73)低于对照组(8.63±0.30,P<0.05),各实验组给予不同浓度Na HS刺激后能够浓度依赖性的抑制这种肥大效应(P<0.05),同时上调了PI3K/AKt/e NOS通路各信号分子的表达量(P<0.05)。结论 H2S对ET-1诱导的心肌肥大有一定的抑制作用,这种作用可能与激活PI3K-AKt-e NOS信号通路有关。展开更多
文摘随着我国乡村振兴战略的实施,农村青少年科普教育的重要性日益凸显。本文从农村青少年科普教育的现状分析入手,探讨了在乡村振兴战略下,如何加强农村青少年科普教育的路径。一是优化农村科普教育环境,创新科普教育模式和方法,加大对农村青少年科普教育的投入,利用现代信息技术,打破地域限制,营造农村科普教育氛围。二是加强农村教师队伍建设和培训,充分调动农村教师队伍的积极性和主动性,提高农村教师整体素质。三是建立全方位的支持体系,出台相关政策,为农村青少年提供更多优质科普资源。本文旨在为乡村振兴战略下加强农村科普教育提供理论指导和实践参考。With the implementation of China’s rural revitalization strategy, the importance of popular science education for rural teenagers has become increasingly prominent. Based on the analysis of the current situation of popular science education for rural teenagers, this paper discusses how to strengthen the popular science education for rural teenagers under the strategy of rural revitalization. The first step is to optimize the environment of rural popular science education, innovate the modes and methods of popular science education, increase investment in rural teenager popular science education, use modern information technology, break geographical restrictions, and create an atmosphere of rural popular science education. The second is to strengthen the construction and training of rural teachers, fully mobilize the enthusiasm and initiative of rural teachers and improve the overall quality of rural teachers. The third is to establish an all-round support system and introduce relevant policies to provide more high-quality popular science resources for rural teenagers. This paper aims to provide theoretical guidance and practical reference for strengthening rural popular science education under the strategy of rural revitalization.
文摘目的观察磷脂酰肌醇-3激酶(PI3K)-蛋白质丝氨酸/苏氨酸激酶(AKt)-内皮型一氧化氮合酶(e NOS)信号转导通路在硫化氢(H2S)抑制内皮素-1(endothelin-1,ET-1)诱导心肌肥大过程中的作用。方法体外培养原代心肌细胞,将其随机分为6组,每组4孔,1对照组:加入等体积无血清的DMEM培养基;2肥大(ET-1)组:加入终浓度为10-8 mol/L的ET-1;剩余4组为实验组,各组分别加入不同终浓度的H2S供体-Na HS:310-15 M Na HS组:加入10-15 mol/L Na HS+10-8 mol/l ET-1;410-14 M Na HS组:加入10-14 mol/L Na HS+10-8 mol/L ET-1;510-13 M Na HS组:加入10-13 mol/L Na HS+10-8 mol/L ET-1;610-12 M Na HS组:加入10-12 mol/L Na HS+10-8 mol/L ET-1。上述各组药物分别刺激24 h后测定心肌细胞表面积、细胞总蛋白含量、培养液NO含量,RT-PCR检测心肌细胞心房利钠肽(atrial natriuretic peptide,ANP)、脑钠肽(B-type natriuretic peptide,BNP)、磷脂酰肌醇-3激酶(phosphatidylinositol-3-kinase,PI3K)、蛋白激酶B(protein kinase B,PKB/AKt)、e NOS m RNA水平,Western Blot技术检测总AKt和磷酸化AKt蛋白表达含量。结果肥大(ET-1)组的心肌细胞表面积(1933.80±143.06)和细胞总蛋白含量(367.51±25.9)均高于对照组(787.27±107.66,218.55±21.28,P<0.05),ANP及BNP m RNA的表达量也明显增加(P<0.05),但PI3K、AKt、e NOS m RNA表达水平,磷酸化AKt程度和NO的释放量(4.60±0.73)低于对照组(8.63±0.30,P<0.05),各实验组给予不同浓度Na HS刺激后能够浓度依赖性的抑制这种肥大效应(P<0.05),同时上调了PI3K/AKt/e NOS通路各信号分子的表达量(P<0.05)。结论 H2S对ET-1诱导的心肌肥大有一定的抑制作用,这种作用可能与激活PI3K-AKt-e NOS信号通路有关。