Objective This study was designed to explore whether Tanshinone ⅡA (TanⅡA) could induce HL-60 and K562 cells differentiation and to assay the telomerase ativities before and after the leukemia cell differentiation i...Objective This study was designed to explore whether Tanshinone ⅡA (TanⅡA) could induce HL-60 and K562 cells differentiation and to assay the telomerase ativities before and after the leukemia cell differentiation induced by TanⅡA and All-trans retinoid acid (ATRA). Methods HL-60 and K562 cells were cultured with TanⅡA at concentration of 0.5μg/ml for 5 days and 6 days respectively. The cell differentiations were observed by morphology and nitroblue tetrazodium (NBT) test. The telomerase activities before and after TanⅡA and ATRA treatment were measured by PCR-TRAP, using ATRA as positive control, 0.01% DMSO as negative control. Results The results showed that TanⅡA inhibited both HL-60 and K562 cell growth. On the 5th day of TanⅡA action, the inhibiting rate of HL-60 cell growth was 79.9%. Also, it induced the cells to differentiate to terminal cells. Acted by TanⅡA for 5 days, the HL-60 cells differentiation rate was 72%, NBT test was positive and showed that the leukemia cells were differentiated. On the 6th day of TanⅡA action, the K562 cells differentiate rate was 76%. The telomerase activities decreased markedly; the inhibiting rates of HL-60 (5 days) and K562 (6 days) were 30.8% and 50.8% respectively. Conclusion These results indicate that TanⅡA induces leukemia cells to differentiate, and with the induction of leukemia cell differentiation, the telomerase activities decrease obviously.展开更多
文摘Objective This study was designed to explore whether Tanshinone ⅡA (TanⅡA) could induce HL-60 and K562 cells differentiation and to assay the telomerase ativities before and after the leukemia cell differentiation induced by TanⅡA and All-trans retinoid acid (ATRA). Methods HL-60 and K562 cells were cultured with TanⅡA at concentration of 0.5μg/ml for 5 days and 6 days respectively. The cell differentiations were observed by morphology and nitroblue tetrazodium (NBT) test. The telomerase activities before and after TanⅡA and ATRA treatment were measured by PCR-TRAP, using ATRA as positive control, 0.01% DMSO as negative control. Results The results showed that TanⅡA inhibited both HL-60 and K562 cell growth. On the 5th day of TanⅡA action, the inhibiting rate of HL-60 cell growth was 79.9%. Also, it induced the cells to differentiate to terminal cells. Acted by TanⅡA for 5 days, the HL-60 cells differentiation rate was 72%, NBT test was positive and showed that the leukemia cells were differentiated. On the 6th day of TanⅡA action, the K562 cells differentiate rate was 76%. The telomerase activities decreased markedly; the inhibiting rates of HL-60 (5 days) and K562 (6 days) were 30.8% and 50.8% respectively. Conclusion These results indicate that TanⅡA induces leukemia cells to differentiate, and with the induction of leukemia cell differentiation, the telomerase activities decrease obviously.
