目的观察神经生长因子(nerve growth factor,NGF)滴眼液对兔眼准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)后角膜神经修复的影响。方法35只中华大耳白兔双眼接受近视性LASIK术,术后左眼用配置的100·g/mlNGF滴眼...目的观察神经生长因子(nerve growth factor,NGF)滴眼液对兔眼准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)后角膜神经修复的影响。方法35只中华大耳白兔双眼接受近视性LASIK术,术后左眼用配置的100·g/mlNGF滴眼液,右眼滴平衡液(balanced salt solution,BSS)作为对照,1滴/次,3次/d。分别于术后第1天、第3天、第1周、第2周、第1个月、第3个月、第6个月各取5只兔,在观察双眼前后节反应后处死兔,摘除双侧角膜,进行角膜神经氯化金染色。光镜下观察LASIK术后角膜神经修复过程,分别比较角膜上皮、浅基质层,深基质层NGF组与BSS组LASIK术后不同时期角膜神经的数量。结果LASIK术后角膜深基质层、角膜瓣连接处的上皮下和浅基质层神经未受损,瓣切削处上皮下和浅基质层神经消失。术后不同部位的角膜神经再生程度不同,术后第6个月周边部角膜神经形态已接近正常,角膜中央仍无神经分布。NGF组与BSS组术后各时间点角膜上皮、浅基质层神经数量比较,NGF组大于BSS组,差异有显著性(P<0.05);两组术后深基质层神经数量比较,NGF组大于BSS组,差异也有显著性(P<0.05)。结论NGF滴眼液对LASIK术后不同部位、不同时期角膜神经的修复有明显促进作用。展开更多
Objective To evaluate the effects of preoperative soft contact lens (SCL) wear on higher-order aberrations(HOAs)correction after wavefront-guided and conventional laser in situ keratomileusis (LASIK). Methods A prospe...Objective To evaluate the effects of preoperative soft contact lens (SCL) wear on higher-order aberrations(HOAs)correction after wavefront-guided and conventional laser in situ keratomileusis (LASIK). Methods A prospective randomized double-masked comparative study was conducted in 180 eyes of 90 patients scheduled for LASIK, including 92 eyes of 46 patients with previous SCL wear. The patients were divided into four groups: no SCL-conventional group, SCL-conventional group, no SCL-wavefront group and SCL-wavefront group. Preoperative and postoperative examinations included uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), contrast sensitivity and HOAs. Results The UCVA and BSCVA were 20/20 in all patients at 6 months postoperatively. And 36.3%, 22.7%, 18.1% and 35.4% gained one line or more of BSCVA in no SCL-wavefront group, SCL-wavefront group, no SCL-conventional group and SCL-conventional group, respectively. Both the safety index and efficacy index surpassed one in 4 groups and were highest in no SCL-wavefront group. The increase in root-mean-square (RMS) values of HOAs was significantly lower in no SCL-wavefront group and higher in SCL-wavefront group. At 6 months postoperatively, the increase in RMS of HOAs (RMSh) were 0.67, 1.58, 1.17 and 1.28 in no SCL-wavefront group, SCL-wavefront group, no SCL-conventional group and SCL-conventional group, respectively. Conclusion Preoperative contact lens wear has significant influence on the efficacy of HOAs correction in wavefront-guided LASIK. Patients with contact lens wear preoperatively should be cautious when scheduled for wavefront-guided LASIK.展开更多
文摘目的观察神经生长因子(nerve growth factor,NGF)滴眼液对兔眼准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)后角膜神经修复的影响。方法35只中华大耳白兔双眼接受近视性LASIK术,术后左眼用配置的100·g/mlNGF滴眼液,右眼滴平衡液(balanced salt solution,BSS)作为对照,1滴/次,3次/d。分别于术后第1天、第3天、第1周、第2周、第1个月、第3个月、第6个月各取5只兔,在观察双眼前后节反应后处死兔,摘除双侧角膜,进行角膜神经氯化金染色。光镜下观察LASIK术后角膜神经修复过程,分别比较角膜上皮、浅基质层,深基质层NGF组与BSS组LASIK术后不同时期角膜神经的数量。结果LASIK术后角膜深基质层、角膜瓣连接处的上皮下和浅基质层神经未受损,瓣切削处上皮下和浅基质层神经消失。术后不同部位的角膜神经再生程度不同,术后第6个月周边部角膜神经形态已接近正常,角膜中央仍无神经分布。NGF组与BSS组术后各时间点角膜上皮、浅基质层神经数量比较,NGF组大于BSS组,差异有显著性(P<0.05);两组术后深基质层神经数量比较,NGF组大于BSS组,差异也有显著性(P<0.05)。结论NGF滴眼液对LASIK术后不同部位、不同时期角膜神经的修复有明显促进作用。
文摘Objective To evaluate the effects of preoperative soft contact lens (SCL) wear on higher-order aberrations(HOAs)correction after wavefront-guided and conventional laser in situ keratomileusis (LASIK). Methods A prospective randomized double-masked comparative study was conducted in 180 eyes of 90 patients scheduled for LASIK, including 92 eyes of 46 patients with previous SCL wear. The patients were divided into four groups: no SCL-conventional group, SCL-conventional group, no SCL-wavefront group and SCL-wavefront group. Preoperative and postoperative examinations included uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), contrast sensitivity and HOAs. Results The UCVA and BSCVA were 20/20 in all patients at 6 months postoperatively. And 36.3%, 22.7%, 18.1% and 35.4% gained one line or more of BSCVA in no SCL-wavefront group, SCL-wavefront group, no SCL-conventional group and SCL-conventional group, respectively. Both the safety index and efficacy index surpassed one in 4 groups and were highest in no SCL-wavefront group. The increase in root-mean-square (RMS) values of HOAs was significantly lower in no SCL-wavefront group and higher in SCL-wavefront group. At 6 months postoperatively, the increase in RMS of HOAs (RMSh) were 0.67, 1.58, 1.17 and 1.28 in no SCL-wavefront group, SCL-wavefront group, no SCL-conventional group and SCL-conventional group, respectively. Conclusion Preoperative contact lens wear has significant influence on the efficacy of HOAs correction in wavefront-guided LASIK. Patients with contact lens wear preoperatively should be cautious when scheduled for wavefront-guided LASIK.
