目的比较0.45%氯化钠液与灭菌注射用水人工气道湿化的效果，从而更好地指导临床。方法计算机检索Medline、EMBase、Cochrane图书馆，CBM、CNKI、万方、维普等文献数据库，检索近10年的文献，并追查相关文献的参考文献。纳入0.45%氯化钠...目的比较0.45%氯化钠液与灭菌注射用水人工气道湿化的效果，从而更好地指导临床。方法计算机检索Medline、EMBase、Cochrane图书馆，CBM、CNKI、万方、维普等文献数据库，检索近10年的文献，并追查相关文献的参考文献。纳入0.45%氯化钠液与灭菌注射用水人工气道湿化的效果的临床对照研究。由2名评价者共同评价所纳入研究的质量。对符合纳入标准的研究应用Rev Man 5.0软件进行Meta分析及数据处理。结果共纳入符合要求的临床对照研究5个，共包括病例330例。 Meta分析结果显示：0.45%氯化钠液气道湿化效果与灭菌注射用水相当，RR=0.90（0.74-1.09），差异无统计学意义（P〉0.05）；0.45%氯化钠液气道湿化后在刺激性咳嗽、气道黏膜出血、肺部感染的不良事件发生率均低于灭菌注射用水，差异有统计学意义（P〈0.05）。结论0.45%氯化钠液气道湿化效果与灭菌注射用水相当，但不良事件发生率低。0.45%氯化钠液更适合用于气道湿化。展开更多
The osteogenic in vitro effect of low intensity pulsed ultrasound (LIPUS) on SD rat adi-pose-derived stem cells (ADSCs) was investigated.Rat ADSCs underwent LIPUS (intensity=100 mW/cm2) or sham exposure for 8 min per ...The osteogenic in vitro effect of low intensity pulsed ultrasound (LIPUS) on SD rat adi-pose-derived stem cells (ADSCs) was investigated.Rat ADSCs underwent LIPUS (intensity=100 mW/cm2) or sham exposure for 8 min per treatment once everyday in vitro,and then the alkaline phos-phatase (ALP) activity and mineralized nodule formation were assessed to evaluate the osteogenic effect of LIPUS on ADSCs.To further explore the underlying mechanism,the osteogenic-related gene mRNA expression was determined by using reverse transcriptase-polymerase chain reaction (RT-PCR) at 1st,3rd,5th,7th day after exposure repectively.Westen blot was used to evaluate the protein expression levels of two osteogenic differentiation associated genes at 7th and 14th day repectively.It was found that ALP activity was increased after LIPUS exposure and LIPUS resulted in mineralized nodule formation of ADSCs in vitro.LIPUS-treated ADSCs displayed higher mRNA expression levels of runt-related transcription factor 2 (Runx2),osteocalcin (OCN),ALP and bone sialoprotein (BSP) genes than con-trols,and the protein levels of Runx2 and BSP were also increased.The results suggested that LIPUS may induce the osteogenic differentiation of ADSCs in vitro.展开更多
文摘目的比较0.45%氯化钠液与灭菌注射用水人工气道湿化的效果，从而更好地指导临床。方法计算机检索Medline、EMBase、Cochrane图书馆，CBM、CNKI、万方、维普等文献数据库，检索近10年的文献，并追查相关文献的参考文献。纳入0.45%氯化钠液与灭菌注射用水人工气道湿化的效果的临床对照研究。由2名评价者共同评价所纳入研究的质量。对符合纳入标准的研究应用Rev Man 5.0软件进行Meta分析及数据处理。结果共纳入符合要求的临床对照研究5个，共包括病例330例。 Meta分析结果显示：0.45%氯化钠液气道湿化效果与灭菌注射用水相当，RR=0.90（0.74-1.09），差异无统计学意义（P〉0.05）；0.45%氯化钠液气道湿化后在刺激性咳嗽、气道黏膜出血、肺部感染的不良事件发生率均低于灭菌注射用水，差异有统计学意义（P〈0.05）。结论0.45%氯化钠液气道湿化效果与灭菌注射用水相当，但不良事件发生率低。0.45%氯化钠液更适合用于气道湿化。
文摘The osteogenic in vitro effect of low intensity pulsed ultrasound (LIPUS) on SD rat adi-pose-derived stem cells (ADSCs) was investigated.Rat ADSCs underwent LIPUS (intensity=100 mW/cm2) or sham exposure for 8 min per treatment once everyday in vitro,and then the alkaline phos-phatase (ALP) activity and mineralized nodule formation were assessed to evaluate the osteogenic effect of LIPUS on ADSCs.To further explore the underlying mechanism,the osteogenic-related gene mRNA expression was determined by using reverse transcriptase-polymerase chain reaction (RT-PCR) at 1st,3rd,5th,7th day after exposure repectively.Westen blot was used to evaluate the protein expression levels of two osteogenic differentiation associated genes at 7th and 14th day repectively.It was found that ALP activity was increased after LIPUS exposure and LIPUS resulted in mineralized nodule formation of ADSCs in vitro.LIPUS-treated ADSCs displayed higher mRNA expression levels of runt-related transcription factor 2 (Runx2),osteocalcin (OCN),ALP and bone sialoprotein (BSP) genes than con-trols,and the protein levels of Runx2 and BSP were also increased.The results suggested that LIPUS may induce the osteogenic differentiation of ADSCs in vitro.
