目的探讨分形趋化因子(FKN)对H9C2心肌细胞炎症因子、细胞凋亡的影响。方法采用(100、200、300、400、500)ng/m L的可溶性FKN(s FKN)处理H9C2细胞0、12、24、36、48 h,利用MTT法检测s FKN对H9C2细胞增殖的作用。筛选出200 ng/m L s FKN...目的探讨分形趋化因子(FKN)对H9C2心肌细胞炎症因子、细胞凋亡的影响。方法采用(100、200、300、400、500)ng/m L的可溶性FKN(s FKN)处理H9C2细胞0、12、24、36、48 h,利用MTT法检测s FKN对H9C2细胞增殖的作用。筛选出200 ng/m L s FKN作用H9C2细胞24 h后,实时定量PCR和Western blot法分别检测细胞肿瘤坏死因子α(TNF-α)mRNA和蛋白的表达,筛选出s FKN最佳处理剂量和处理时间。采用200 ng/m L s FKN,200 ng/m L s FKN与5 ng/m L AKT抑制剂LY294002分别处理H9C2细胞24 h,通过Hochest33258染色检测细胞核染色质聚集情况。采用实时定量PCR和Western blot法分别检测H9C2细胞中Bax和Bcl-2的表达。结果 s FKN可以抑制H9C2细胞的增殖情况。FKN促进心肌细胞TNF-α的表达,具有剂量依赖性。FKN可通过上调Bax蛋白的表达与下调Bcl-2蛋白的表达促进心肌细胞凋亡。FKN可能通过激活PI3K/AKT通路促进心肌细胞的炎症发生与凋亡。结论 FKN通过激活PI3K/AKT通路促进心肌细胞的炎症反应和凋亡作用。展开更多
Bacground Percutaneous coronary intervention（PCI） has become one of the most effective treatments in coronary heart disease（CHD）.However,the bottleneck problem of PCI is the in-stent restenosis（ISR）.The aim of t...Bacground Percutaneous coronary intervention（PCI） has become one of the most effective treatments in coronary heart disease（CHD）.However,the bottleneck problem of PCI is the in-stent restenosis（ISR）.The aim of this study was to explore the effects of astragaloside IV（AST IV） on suppression of intimal hyperplasia modulation of the expression of basic fibroblast growth factor（b-FGF） in a rat carotid artery balloon injury model.Methods Fifty healthy male Sprague-Dawley（SD）rats were randomly divided into five groups：a sham-operation group（sham）,a model group（model）,and three astragaloside IV-treated groups.Three days before the surgery,1% carboxy methyl cellulose（CMC） or AST IV（20,40 or 60 mg·kg^（- 1）·d^（- 1）） was intragastrically administered into sham or 3 astragaloside-treated groups once a day for 17 days.Hematoxylin-elsin staining was carried out to determine the pathomorphological changes and the neointimal and media area ratio.Immunohistochemistry staining was performed to measure the expressions of proliferating cell nuclear antigen（PCNA）and basic fibrolast growth factor（b-FGF）.PCNA and b-FGF were analyzed with Iamage-Pro Plus.Results（1） The carotid artery intimal hyperplasia in the rats of model was similar to lumen stenosis.Compared with the sham operation group,the area of the new intima and the ratio of the intima to media（I/M） were increased and the lumen area was decreased（P 〈 0.01） in the model group.Astragaloside IV increased the lumen intimal dimension and decreased the area of new intima and the ratio of intima to media in a dose-dependent manner.（2） Compared with the sham-operation group,the expressions of PCNA and b-FGF in carotid artery of model group were significantly increased（P 〈 0.01）.AST IV decreased expressions of PCNA and b-FGF in the carotid artery of rats in a dosedependent manner.Conclusion Astragaloside IV significantly inhibits neointimal hyperplasia of rat carotid artery through down-regulating the expressions of PCNA and b-FGF.展开更多
文摘目的探讨分形趋化因子(FKN)对H9C2心肌细胞炎症因子、细胞凋亡的影响。方法采用(100、200、300、400、500)ng/m L的可溶性FKN(s FKN)处理H9C2细胞0、12、24、36、48 h,利用MTT法检测s FKN对H9C2细胞增殖的作用。筛选出200 ng/m L s FKN作用H9C2细胞24 h后,实时定量PCR和Western blot法分别检测细胞肿瘤坏死因子α(TNF-α)mRNA和蛋白的表达,筛选出s FKN最佳处理剂量和处理时间。采用200 ng/m L s FKN,200 ng/m L s FKN与5 ng/m L AKT抑制剂LY294002分别处理H9C2细胞24 h,通过Hochest33258染色检测细胞核染色质聚集情况。采用实时定量PCR和Western blot法分别检测H9C2细胞中Bax和Bcl-2的表达。结果 s FKN可以抑制H9C2细胞的增殖情况。FKN促进心肌细胞TNF-α的表达,具有剂量依赖性。FKN可通过上调Bax蛋白的表达与下调Bcl-2蛋白的表达促进心肌细胞凋亡。FKN可能通过激活PI3K/AKT通路促进心肌细胞的炎症发生与凋亡。结论 FKN通过激活PI3K/AKT通路促进心肌细胞的炎症反应和凋亡作用。
基金supported by Natural Scientific Foundation of Shandong Province of China(No.ZR2011HL006)
文摘Bacground Percutaneous coronary intervention（PCI） has become one of the most effective treatments in coronary heart disease（CHD）.However,the bottleneck problem of PCI is the in-stent restenosis（ISR）.The aim of this study was to explore the effects of astragaloside IV（AST IV） on suppression of intimal hyperplasia modulation of the expression of basic fibroblast growth factor（b-FGF） in a rat carotid artery balloon injury model.Methods Fifty healthy male Sprague-Dawley（SD）rats were randomly divided into five groups：a sham-operation group（sham）,a model group（model）,and three astragaloside IV-treated groups.Three days before the surgery,1% carboxy methyl cellulose（CMC） or AST IV（20,40 or 60 mg·kg^（- 1）·d^（- 1）） was intragastrically administered into sham or 3 astragaloside-treated groups once a day for 17 days.Hematoxylin-elsin staining was carried out to determine the pathomorphological changes and the neointimal and media area ratio.Immunohistochemistry staining was performed to measure the expressions of proliferating cell nuclear antigen（PCNA）and basic fibrolast growth factor（b-FGF）.PCNA and b-FGF were analyzed with Iamage-Pro Plus.Results（1） The carotid artery intimal hyperplasia in the rats of model was similar to lumen stenosis.Compared with the sham operation group,the area of the new intima and the ratio of the intima to media（I/M） were increased and the lumen area was decreased（P 〈 0.01） in the model group.Astragaloside IV increased the lumen intimal dimension and decreased the area of new intima and the ratio of intima to media in a dose-dependent manner.（2） Compared with the sham-operation group,the expressions of PCNA and b-FGF in carotid artery of model group were significantly increased（P 〈 0.01）.AST IV decreased expressions of PCNA and b-FGF in the carotid artery of rats in a dosedependent manner.Conclusion Astragaloside IV significantly inhibits neointimal hyperplasia of rat carotid artery through down-regulating the expressions of PCNA and b-FGF.
