[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,...[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,Selenomonas ruminantium（recipient strain）was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.[Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.[Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal.展开更多
目的探讨不同液体复苏对院前抢救创伤非控制出血性休克的影响。方法选择创伤非控制出血性休克237例，其中A组117例，2010-01-2011-10期间，采用限制性液体复苏行院前抢救；B组120例，选取既往患者，当时采用充分液体复苏行院前抢救。比...目的探讨不同液体复苏对院前抢救创伤非控制出血性休克的影响。方法选择创伤非控制出血性休克237例，其中A组117例，2010-01-2011-10期间，采用限制性液体复苏行院前抢救；B组120例，选取既往患者，当时采用充分液体复苏行院前抢救。比较两组输液量，病死率及并发症情况。结果院前急救时，与B组比较，限制性液体复苏组（A组）输液量少，[（650±310）mL vs （2580±720）mL]，病死率低（21．37％vs34．99％），凝血酶原时间（胛）短[（11．17±1．5）SVS（16．78±1．96）s]，并发症[如急性。肾衰竭（ARF）、弥散性血管内凝血（DIC）、成人呼吸窘迫综合征（ARDS）及多脏器功能障碍综合征（MODS）等]发生率合计低（16．30％VS37．18％），两组差异均有统计学意义（P〈0．05）。结论院前急救采用限制性液体复苏能减少输液量，降低病死率，存活者并发症发生率低，适宜临床救治。展开更多
基金Supported by National Natural Science Fund Item(30230260,30600441)~~
文摘[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,Selenomonas ruminantium（recipient strain）was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.[Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.[Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal.
文摘目的探讨不同液体复苏对院前抢救创伤非控制出血性休克的影响。方法选择创伤非控制出血性休克237例，其中A组117例，2010-01-2011-10期间，采用限制性液体复苏行院前抢救；B组120例，选取既往患者，当时采用充分液体复苏行院前抢救。比较两组输液量，病死率及并发症情况。结果院前急救时，与B组比较，限制性液体复苏组（A组）输液量少，[（650±310）mL vs （2580±720）mL]，病死率低（21．37％vs34．99％），凝血酶原时间（胛）短[（11．17±1．5）SVS（16．78±1．96）s]，并发症[如急性。肾衰竭（ARF）、弥散性血管内凝血（DIC）、成人呼吸窘迫综合征（ARDS）及多脏器功能障碍综合征（MODS）等]发生率合计低（16．30％VS37．18％），两组差异均有统计学意义（P〈0．05）。结论院前急救采用限制性液体复苏能减少输液量，降低病死率，存活者并发症发生率低，适宜临床救治。
