目的探讨女童初潮年龄与成人体重指数(BMI)的关系及关键基因、通路。方法从IEU Open GWAS数据库中得到初潮年龄和成人BMI的汇总统计数据集,将初潮年龄作为暴露因素,成人BMI为结局变量。采用孟德尔随机化方法分析初潮年龄与成人BMI的因...目的探讨女童初潮年龄与成人体重指数(BMI)的关系及关键基因、通路。方法从IEU Open GWAS数据库中得到初潮年龄和成人BMI的汇总统计数据集,将初潮年龄作为暴露因素,成人BMI为结局变量。采用孟德尔随机化方法分析初潮年龄与成人BMI的因果关系。使用Gene Cards、Pharm GKB数据库分析初潮年龄和成人BMI的交集靶点,Cytoscape软件构建核心网络,Metascape在线数据库进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)通路分析。结果随机效应逆方差加权(IVW)法结果显示,初潮年龄与成人BMI呈负相关(OR=0.812,P<0.001);加权中位数法、加权模式法支持此结果。敏感性分析结果显示,结果存在一定的异质性,但本研究使用随机效应IVW法,对结果无影响;结果不存在多效性;不存在某个单核苷酸多态性单独对因果关系产生显著影响。初潮年龄与成人BMI交集靶点共765个,核心靶点为MAPK1、MAPK3、AKT1等。GO富集分析发现,生物学过程包含对激素的反应、激素水平调节等,细胞组成包含受体复合物、神经元胞体等,分子功能包含信号受体调节剂活性、受体配体活性等。KEGG通路分析发现,通路涉及神经活性配体-受体相互作用、TGF-β信号通路、卵巢类固醇生成等。结论遗传预测女童初潮年龄和成人BMI存在负向因果关联,可能通过MAPK1等关键基因,神经活性相关信号通路等关键通路发挥作用。展开更多
随着近些年中国经济迅猛发展,国际地位的不断攀升以及医学高等教育的国际化,来华求学的留学生人数逐年增多。如何提高留学生的教学质量是当前每个医学院校的首要任务。扎实的人体寄生虫学知识是完善留学生医学知识体系、通过美国执业医...随着近些年中国经济迅猛发展,国际地位的不断攀升以及医学高等教育的国际化,来华求学的留学生人数逐年增多。如何提高留学生的教学质量是当前每个医学院校的首要任务。扎实的人体寄生虫学知识是完善留学生医学知识体系、通过美国执业医师考试USMLE (United States Medical Licensing Examination)不可或缺的条件。为了准确、客观地评价留学生的人体寄生虫课程学习效果,石河子大学医学院病原免疫教研室构建了留学生人体寄生虫考试试题库。试题库不仅是检验留学生学习效果的重要手段,也是实现教考分离的举措之一;试题库极大地提升了阅卷效率并能有效地减少误判率。构建留学生试题库是医学教育考试、管理从传统向现代化、科学化、规范化过渡的必经之路。本文从试题建设的导向、和临床结合的状况、题型的设置和比例、题目的数量、分值的设置、题型难易比例、答题时间等介绍本教研室留学生人体寄生虫试题库建设的体会,以期为留学生适应、通过USMLE考试提供帮助。With the rapid development of China’s economy in recent years, the continuous rise of China’s international status and the internationalization of medical higher education, the number of international students studying in China is increasing year by year. How to improve the teaching quality of international students is the primary task of every medical college. Solid knowledge of human parasitology is an indispensable condition for international students to improve their medical knowledge system and pass the United States Medical Licensing Examination (USMLE). In order to accurately and objectively evaluate the learning effect of the human parasite course for international students, the Department of Pathogen and Immunity of the School of Medicine of Shihezi University established the test bank of human parasite examination for international students. The test bank is not only an important means to check the learning effect of international students, but also one of the measures to realize the separation of teaching and examination. The test bank greatly improves the marking efficiency and can effectively reduce the misjudgment rate. It is the only way for medical education examination and management to transition from traditional to modern, scientific and standardized. This paper introduces the experience of the construction of human parasite test bank for international students in the teaching and Research Department from the guidance of test construction, the situation of combining with clinical practice, the setting and proportion of question types, the number of questions, the setting of score, the ratio of difficulty and ease of question types, and the answering time, so as to provide help for international students to adapt to and pass the USMLE examination.展开更多
为了探明传统自然发酵豆瓣不同时期微生物的多样性的差异对豆瓣风味品质的影响,采用纯培养的分离方法,对发酵中期(5个月)时期和成熟期(10个月)豆瓣进行了细菌16S r RNA和真菌18S r RNA的基因分析。结果表明,这两个时期豆瓣中的微生物种...为了探明传统自然发酵豆瓣不同时期微生物的多样性的差异对豆瓣风味品质的影响,采用纯培养的分离方法,对发酵中期(5个月)时期和成熟期(10个月)豆瓣进行了细菌16S r RNA和真菌18S r RNA的基因分析。结果表明,这两个时期豆瓣中的微生物种类丰富,且差异很大。从发酵五个月的豆瓣中分离得到7个属(Bacillus、Oceanobacillus、Virgibacillus、Lactobacillus、Pichia、Candida、Wickerhamomyces),优势菌为Bacillus、Candida、Wickerhamomyces属,分别占54%、11%、14%。成熟期中分离得到8个属(Bacillus、Halomonas、Oceanobacillus、Virgibacillus、Lactobacillus、Gracilibacillus、Pichia、Aspergillus属),优势菌为Bacillus、Oceanobacillus、Halomona属,分别占55%、16%、10%。展开更多
以一级郫县豆瓣为原料筛选磷脂酶高产菌株,通过磷脂平板初筛和摇瓶复筛法,以磷脂酶水解圈作为筛选模型,并对产酶菌株进行了酶活力的检测及细菌16S r RNA系统发育分析。结果表明,从一级豆瓣中筛选出10株产磷脂酶菌株,其中产酶活力最高的...以一级郫县豆瓣为原料筛选磷脂酶高产菌株,通过磷脂平板初筛和摇瓶复筛法,以磷脂酶水解圈作为筛选模型,并对产酶菌株进行了酶活力的检测及细菌16S r RNA系统发育分析。结果表明,从一级豆瓣中筛选出10株产磷脂酶菌株,其中产酶活力最高的为编号LZ8,酶活力为68.32 U/m L,经鉴定该菌株为Bacillus sonorensis,属于芽孢杆菌属,其与最高同源性菌株相似性为100%。展开更多
文摘目的探讨女童初潮年龄与成人体重指数(BMI)的关系及关键基因、通路。方法从IEU Open GWAS数据库中得到初潮年龄和成人BMI的汇总统计数据集,将初潮年龄作为暴露因素,成人BMI为结局变量。采用孟德尔随机化方法分析初潮年龄与成人BMI的因果关系。使用Gene Cards、Pharm GKB数据库分析初潮年龄和成人BMI的交集靶点,Cytoscape软件构建核心网络,Metascape在线数据库进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)通路分析。结果随机效应逆方差加权(IVW)法结果显示,初潮年龄与成人BMI呈负相关(OR=0.812,P<0.001);加权中位数法、加权模式法支持此结果。敏感性分析结果显示,结果存在一定的异质性,但本研究使用随机效应IVW法,对结果无影响;结果不存在多效性;不存在某个单核苷酸多态性单独对因果关系产生显著影响。初潮年龄与成人BMI交集靶点共765个,核心靶点为MAPK1、MAPK3、AKT1等。GO富集分析发现,生物学过程包含对激素的反应、激素水平调节等,细胞组成包含受体复合物、神经元胞体等,分子功能包含信号受体调节剂活性、受体配体活性等。KEGG通路分析发现,通路涉及神经活性配体-受体相互作用、TGF-β信号通路、卵巢类固醇生成等。结论遗传预测女童初潮年龄和成人BMI存在负向因果关联,可能通过MAPK1等关键基因,神经活性相关信号通路等关键通路发挥作用。
文摘随着近些年中国经济迅猛发展,国际地位的不断攀升以及医学高等教育的国际化,来华求学的留学生人数逐年增多。如何提高留学生的教学质量是当前每个医学院校的首要任务。扎实的人体寄生虫学知识是完善留学生医学知识体系、通过美国执业医师考试USMLE (United States Medical Licensing Examination)不可或缺的条件。为了准确、客观地评价留学生的人体寄生虫课程学习效果,石河子大学医学院病原免疫教研室构建了留学生人体寄生虫考试试题库。试题库不仅是检验留学生学习效果的重要手段,也是实现教考分离的举措之一;试题库极大地提升了阅卷效率并能有效地减少误判率。构建留学生试题库是医学教育考试、管理从传统向现代化、科学化、规范化过渡的必经之路。本文从试题建设的导向、和临床结合的状况、题型的设置和比例、题目的数量、分值的设置、题型难易比例、答题时间等介绍本教研室留学生人体寄生虫试题库建设的体会,以期为留学生适应、通过USMLE考试提供帮助。With the rapid development of China’s economy in recent years, the continuous rise of China’s international status and the internationalization of medical higher education, the number of international students studying in China is increasing year by year. How to improve the teaching quality of international students is the primary task of every medical college. Solid knowledge of human parasitology is an indispensable condition for international students to improve their medical knowledge system and pass the United States Medical Licensing Examination (USMLE). In order to accurately and objectively evaluate the learning effect of the human parasite course for international students, the Department of Pathogen and Immunity of the School of Medicine of Shihezi University established the test bank of human parasite examination for international students. The test bank is not only an important means to check the learning effect of international students, but also one of the measures to realize the separation of teaching and examination. The test bank greatly improves the marking efficiency and can effectively reduce the misjudgment rate. It is the only way for medical education examination and management to transition from traditional to modern, scientific and standardized. This paper introduces the experience of the construction of human parasite test bank for international students in the teaching and Research Department from the guidance of test construction, the situation of combining with clinical practice, the setting and proportion of question types, the number of questions, the setting of score, the ratio of difficulty and ease of question types, and the answering time, so as to provide help for international students to adapt to and pass the USMLE examination.
文摘为了探明传统自然发酵豆瓣不同时期微生物的多样性的差异对豆瓣风味品质的影响,采用纯培养的分离方法,对发酵中期(5个月)时期和成熟期(10个月)豆瓣进行了细菌16S r RNA和真菌18S r RNA的基因分析。结果表明,这两个时期豆瓣中的微生物种类丰富,且差异很大。从发酵五个月的豆瓣中分离得到7个属(Bacillus、Oceanobacillus、Virgibacillus、Lactobacillus、Pichia、Candida、Wickerhamomyces),优势菌为Bacillus、Candida、Wickerhamomyces属,分别占54%、11%、14%。成熟期中分离得到8个属(Bacillus、Halomonas、Oceanobacillus、Virgibacillus、Lactobacillus、Gracilibacillus、Pichia、Aspergillus属),优势菌为Bacillus、Oceanobacillus、Halomona属,分别占55%、16%、10%。
