The physiological and anatomical changes in leaves of Poinsettia (Euphorbia pulcherrima) tube_plantlets occurred in the process of transplantation and acclimation were investigated in this paper. Before transplantatio...The physiological and anatomical changes in leaves of Poinsettia (Euphorbia pulcherrima) tube_plantlets occurred in the process of transplantation and acclimation were investigated in this paper. Before transplantation and acclimation, leaves of the tube_plantlets were apparently thin and the vascular system was not well developed, and the net photosynthetic rate of the plantlet was low, but the stomatal conductance and transpiration rate of the leaves were relatively high. At the early stage of transplantation, the tube_plantlet was vulnerable to relative low humidity and intense light that could resulted in wilting of leaves due to excess water loss. During the acclimatization, the net photosynthetic rate of the plantlets increased progressively and the stomatal conductance and transpiration rate decreased gradually, accompanied by an increase in the thickness of the leaves and the size of leaf epidermal cells, together with the fully development of vascular system, palisade and spongy tissues in leaves. The results indicated that the acclimatization could greatly improve the photoautotrophic ability of the tube_plantlets, bring anatomical changes to the plantlets in order to avoid excessive leaf dehydration for the gradual adaptation of the plantlets to natural environment.展开更多
The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the expla...The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.展开更多
文摘The physiological and anatomical changes in leaves of Poinsettia (Euphorbia pulcherrima) tube_plantlets occurred in the process of transplantation and acclimation were investigated in this paper. Before transplantation and acclimation, leaves of the tube_plantlets were apparently thin and the vascular system was not well developed, and the net photosynthetic rate of the plantlet was low, but the stomatal conductance and transpiration rate of the leaves were relatively high. At the early stage of transplantation, the tube_plantlet was vulnerable to relative low humidity and intense light that could resulted in wilting of leaves due to excess water loss. During the acclimatization, the net photosynthetic rate of the plantlets increased progressively and the stomatal conductance and transpiration rate decreased gradually, accompanied by an increase in the thickness of the leaves and the size of leaf epidermal cells, together with the fully development of vascular system, palisade and spongy tissues in leaves. The results indicated that the acclimatization could greatly improve the photoautotrophic ability of the tube_plantlets, bring anatomical changes to the plantlets in order to avoid excessive leaf dehydration for the gradual adaptation of the plantlets to natural environment.
文摘The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.
