[Objective] The aim was to study the tissue culture of mini-Papaya.[Method] The pretreatment seeds of mini-Papaya were cultured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.[Re...[Objective] The aim was to study the tissue culture of mini-Papaya.[Method] The pretreatment seeds of mini-Papaya were cultured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.[Result] In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52%.After seeds which had been soaked by the equi-volume mix-solution of 1 000 mg/L GA3 and 1 mg/L 6-BA for 18 h were further purified in MS medium,the germination rate of seed,the length of embryo bud and radicle and the height of seedling were 68.42%,2.25,0.80 and 1.52 cm respectively,furthermore the total situation of seedling growth was better.When subculture multiplication medium was MS+0.5 mg/L 6-BA+0.1 mg/L IBA medium,proliferation coefficient of subculture multiplication reached the highest (7.92),and the seedlings grew better.The ratio of vitrified shoots decreasing with the increase of light intensity could reach the lowest level (3.21%) under the light intensity of 3 000 lx.[Conclusion] The research provides reference for studies on tissue culture and rapid propagation of mini-Papaya.展开更多
[ Objective ] The study aimed to effectively and timely control the occurrence and damage of Rhyrthophorus femuneus Fabricius in Nanning City, Guangxi Province. [Method]The investigation on the distribution and damage...[ Objective ] The study aimed to effectively and timely control the occurrence and damage of Rhyrthophorus femuneus Fabricius in Nanning City, Guangxi Province. [Method]The investigation on the distribution and damage of R. ferrugineus was conducted in Nanning City, Guangxi Province. Its transmit ways were chocked, light trapping experiment, combination of artificial and chemical control, and chemical control were conducted. [ Result] The investigation indicated that there had distribution of R. ferrugineus in 70% plant nurseries and the scenic areas in Nanning City. Among the planted Hemp palm plants, the damaged vari- eties were up to 80%. The output of palm seedlings which had carried with pests was the main approach for spread and damage of R. ferrugineus. It was effective and feasible to use frequently vibration trapping light to trap adults. Pesticides were sprayed on the bellmouth of host ( central leaf), then aging leaf sheaths or agri- cultural film was used to strap tender leaf sheath in stalks, which could achieve the purpose of controlling R. femugineus. [ Conclusion ] The control strategies of R. femugineus should be focused on controlling seedlings and the plants with tree age less than ten year; the plants with long tree age and the tall plants should adopt selection control if it was necessary, and the integrated control should be implemented on the prevention measure.展开更多
The occurrence periods of Semiaphis heraclei Takahashi,Frankliniella sp.,Haptonchus luteolus and Microsphara linicerae Enchson wint.in Rabenh.causing damage on Flos lonicerae were investigated in F.lonicerae planting ...The occurrence periods of Semiaphis heraclei Takahashi,Frankliniella sp.,Haptonchus luteolus and Microsphara linicerae Enchson wint.in Rabenh.causing damage on Flos lonicerae were investigated in F.lonicerae planting area in XinCheng county of Guangxi Province during 2008-2010,which were coincided with the occurrence periods of related phenology of local Prunus persica Rootstock.With P.persica Rootstock as indicator plant,the occurrence periods of three species of pests and one species of disease were predicted,respectively,and the method was simple and accurate,which could be the foundation for preventing these pests and diseases in the local field.展开更多
[ Objective ] The paper was to study the main diseases and pests in Lonicerae japonica in Guangxi Province and fluctuation pattern of their natural ene- mies. [Method] The population change of main diseases and pests,...[ Objective ] The paper was to study the main diseases and pests in Lonicerae japonica in Guangxi Province and fluctuation pattern of their natural ene- mies. [Method] The population change of main diseases and pests, and main predatory naturaI enemies of pests in different species of Lonicerae japonica in Xincheng County of Guangxi Province were systematically investigated during 2008 -2010. [ Result ] The variation cycle of population number of Semiaphis heraclei (Takahashi) belongs to single peak type, and it has large occurrence in April. The variation cycle of population number of Franklinlella sp. and Haptonchus luteo- ius (Erichson) belongs to double peak type, and they have large occurrence at April and September. The variation cycle of disease severity of Microsphaera lonicerae ( Wint. ) belongs to double peak type, and the disease index will be larger at April and September. The variation cycle of diseased shoot rate belongs to single peak type; diseased shoot rote of Lonicera dasystyla Rehd. and Loniccra hvpolzlauca Miquel is relatively high during April to June, while the index of Lonice- ra confusa (Sweet) DC. is relatively high during February to May. The variation cycle of population number of main natural enemies such as Lemnia biplagiata ( Swartz), Coccinella septcmpunctata ( Linnaeus ) and Harmonia axyridis (Pallas) belongs to double peak type, and they have large occurrence at April and Sep- tember. [ Conclusion] The results could provide scientific basis for determination of optimum control period against major diseases and pests in Loniceraejaponica and development of integrated control programs.展开更多
基金Support by Guangxi Vocational Technical College of Agriculture Scientific Research and Technological Development Projects of China(Agriculture Vocational Department B070210)~~
文摘[Objective] The aim was to study the tissue culture of mini-Papaya.[Method] The pretreatment seeds of mini-Papaya were cultured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.[Result] In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52%.After seeds which had been soaked by the equi-volume mix-solution of 1 000 mg/L GA3 and 1 mg/L 6-BA for 18 h were further purified in MS medium,the germination rate of seed,the length of embryo bud and radicle and the height of seedling were 68.42%,2.25,0.80 and 1.52 cm respectively,furthermore the total situation of seedling growth was better.When subculture multiplication medium was MS+0.5 mg/L 6-BA+0.1 mg/L IBA medium,proliferation coefficient of subculture multiplication reached the highest (7.92),and the seedlings grew better.The ratio of vitrified shoots decreasing with the increase of light intensity could reach the lowest level (3.21%) under the light intensity of 3 000 lx.[Conclusion] The research provides reference for studies on tissue culture and rapid propagation of mini-Papaya.
基金Supported by Natural Science and Technology Fund Project in Guangxi Vocational Technical College of Agriculture(B070206)~~
文摘[ Objective ] The study aimed to effectively and timely control the occurrence and damage of Rhyrthophorus femuneus Fabricius in Nanning City, Guangxi Province. [Method]The investigation on the distribution and damage of R. ferrugineus was conducted in Nanning City, Guangxi Province. Its transmit ways were chocked, light trapping experiment, combination of artificial and chemical control, and chemical control were conducted. [ Result] The investigation indicated that there had distribution of R. ferrugineus in 70% plant nurseries and the scenic areas in Nanning City. Among the planted Hemp palm plants, the damaged vari- eties were up to 80%. The output of palm seedlings which had carried with pests was the main approach for spread and damage of R. ferrugineus. It was effective and feasible to use frequently vibration trapping light to trap adults. Pesticides were sprayed on the bellmouth of host ( central leaf), then aging leaf sheaths or agri- cultural film was used to strap tender leaf sheath in stalks, which could achieve the purpose of controlling R. femugineus. [ Conclusion ] The control strategies of R. femugineus should be focused on controlling seedlings and the plants with tree age less than ten year; the plants with long tree age and the tall plants should adopt selection control if it was necessary, and the integrated control should be implemented on the prevention measure.
基金Supported by Natural Scientific Research Topics of Guangxi Scienceand Technology Department(GKG0992003B-40)Natural Scientific Research Topics of Guangxi Education Department(GJKY200809MS196)~~
文摘The occurrence periods of Semiaphis heraclei Takahashi,Frankliniella sp.,Haptonchus luteolus and Microsphara linicerae Enchson wint.in Rabenh.causing damage on Flos lonicerae were investigated in F.lonicerae planting area in XinCheng county of Guangxi Province during 2008-2010,which were coincided with the occurrence periods of related phenology of local Prunus persica Rootstock.With P.persica Rootstock as indicator plant,the occurrence periods of three species of pests and one species of disease were predicted,respectively,and the method was simple and accurate,which could be the foundation for preventing these pests and diseases in the local field.
基金Supported by Research Projects of Science and Technology Department of Guangxi Zhuang Autonomous Region(GKG0992003B-40)Research Projects of Education Department of Guangxi Zhuang Autonomous Region(GJK[2008]27)~~
文摘[ Objective ] The paper was to study the main diseases and pests in Lonicerae japonica in Guangxi Province and fluctuation pattern of their natural ene- mies. [Method] The population change of main diseases and pests, and main predatory naturaI enemies of pests in different species of Lonicerae japonica in Xincheng County of Guangxi Province were systematically investigated during 2008 -2010. [ Result ] The variation cycle of population number of Semiaphis heraclei (Takahashi) belongs to single peak type, and it has large occurrence in April. The variation cycle of population number of Franklinlella sp. and Haptonchus luteo- ius (Erichson) belongs to double peak type, and they have large occurrence at April and September. The variation cycle of disease severity of Microsphaera lonicerae ( Wint. ) belongs to double peak type, and the disease index will be larger at April and September. The variation cycle of diseased shoot rate belongs to single peak type; diseased shoot rote of Lonicera dasystyla Rehd. and Loniccra hvpolzlauca Miquel is relatively high during April to June, while the index of Lonice- ra confusa (Sweet) DC. is relatively high during February to May. The variation cycle of population number of main natural enemies such as Lemnia biplagiata ( Swartz), Coccinella septcmpunctata ( Linnaeus ) and Harmonia axyridis (Pallas) belongs to double peak type, and they have large occurrence at April and Sep- tember. [ Conclusion] The results could provide scientific basis for determination of optimum control period against major diseases and pests in Loniceraejaponica and development of integrated control programs.