[Objective] This study aimed to investigate the changes of granule struc- ture, characteristic and related enzymatic activity of starch in upper flue-cured tobac- co leaves during bulk flue-curing process, to provide ...[Objective] This study aimed to investigate the changes of granule struc- ture, characteristic and related enzymatic activity of starch in upper flue-cured tobac- co leaves during bulk flue-curing process, to provide theoretical basis for reasonably regulating the starch content in upper flue-cured tobacco leaves and improving the quality of upper flue-cured tobacco leaves. [Method] Changes of amylase activity, enzyme hydrolysability and granule structure of starch in upper flue-cured tobacco leaves were studied during flue-curing process using conventional physical and chemical analysis and SEM. [Result] During the flue-curing process, the starch con- tent of tobacco leaves changed greatly at yellowing stage, and the activity of amy- lase presented the double-peak curve. The SEM observation results showed that most of the starch granules in fresh tobacco leaves were spheroidal and long cylin- drical, while some individuals were in irregular shape, with obvious groove-like in- vagination and layered structure on granule surface; during the flue-curing process, a large amount of starch granules showed layered structure on the surface at yellow- ing stage, and the long cylindrical starch granules were reduced greatly; at the end of flue-curing, there were barely any long cylindrical starch granule in flue-cured to- bacco leaves. Average long-axis-diameter of starch granules in fresh tobacco leaves was 3.21 μm, showing an overall gradually increasing trend during the flue-curing process, which was significantly enhanced at 38 and 42 ℃. The diameter of starch granules increased by more than 60% at the end of 42 ℃ and there was no re- markable difference after 47 ℃. During the flue-curing process, enzyme hydrolysabil- ity of starch in flue-cured tobacco leaves first increased, reached a peak at 38 ~C, and then decreased. [Conclusion] During the bulk flue-curing, the yellowing stage is the critical period for starch content, granule structure and characteristic changes of flue-cured tobacco leaves, it is of positive effects to improve the quality of upper flue-cured tobacco leaves by regulating the flue-curing conditions at yellowing stage.展开更多
Objective The aim was to elucidate the effects of N rates on rice canopy microclimate and community health so as to provide a sci- entific basis for studying the production potential in irrigated rice with healthy can...Objective The aim was to elucidate the effects of N rates on rice canopy microclimate and community health so as to provide a sci- entific basis for studying the production potential in irrigated rice with healthy canopy. Method The effects of rice population structure traits under different N rates on rice canopy temperature, relative humidity （ RH）, light transmittance and sheath blight were studied by using Sunscan canopy analysis system and HOBO（ Pro Temp/RH IS logger）. Result The results showed that leaf area index, plant height and tiller number had significant effects on canopy cooling, RH enhancing and light reducing. Extremely significant multiple linear regression relationships existed among canopy day temperature, day RH, LAI and tiller number, and among light transmittance, tiller number and plant height. At flowering stage, per unit LAI could result in a day-maximum-temperature （Tmax） deceasing of 0.87℃ and a day-minimum-RH （RHmin） enhancing of 2.5% within canopy. Similarly, 100 plants per ms could respectively cause a Tmax deceasing of 1.23℃ and an RHmin enhancing of 3.3% in rice canopy. And per 10 cm plant height and 100 plants per m^2 could respectively reduce 9.3% and 7.8% of light in canopy. Conclusion Sheath blight disease index was significantly enhanced as the canopy day temperature decreased, day RH increased and light transmittance reduced. Bigger canopy from higher nitrogen level treatment leads to a more stable canopy microclimate with little changes in temperature and RH during day and night, which has the risk of worsening canopy health. Thus, moderately controlling the space development of canopy is the basis of constructing healthy canopy in rice.展开更多
基金Supported by Project of State Tobacco Monopoly Administration(TS-01-2011006)~~
文摘[Objective] This study aimed to investigate the changes of granule struc- ture, characteristic and related enzymatic activity of starch in upper flue-cured tobac- co leaves during bulk flue-curing process, to provide theoretical basis for reasonably regulating the starch content in upper flue-cured tobacco leaves and improving the quality of upper flue-cured tobacco leaves. [Method] Changes of amylase activity, enzyme hydrolysability and granule structure of starch in upper flue-cured tobacco leaves were studied during flue-curing process using conventional physical and chemical analysis and SEM. [Result] During the flue-curing process, the starch con- tent of tobacco leaves changed greatly at yellowing stage, and the activity of amy- lase presented the double-peak curve. The SEM observation results showed that most of the starch granules in fresh tobacco leaves were spheroidal and long cylin- drical, while some individuals were in irregular shape, with obvious groove-like in- vagination and layered structure on granule surface; during the flue-curing process, a large amount of starch granules showed layered structure on the surface at yellow- ing stage, and the long cylindrical starch granules were reduced greatly; at the end of flue-curing, there were barely any long cylindrical starch granule in flue-cured to- bacco leaves. Average long-axis-diameter of starch granules in fresh tobacco leaves was 3.21 μm, showing an overall gradually increasing trend during the flue-curing process, which was significantly enhanced at 38 and 42 ℃. The diameter of starch granules increased by more than 60% at the end of 42 ℃ and there was no re- markable difference after 47 ℃. During the flue-curing process, enzyme hydrolysabil- ity of starch in flue-cured tobacco leaves first increased, reached a peak at 38 ~C, and then decreased. [Conclusion] During the bulk flue-curing, the yellowing stage is the critical period for starch content, granule structure and characteristic changes of flue-cured tobacco leaves, it is of positive effects to improve the quality of upper flue-cured tobacco leaves by regulating the flue-curing conditions at yellowing stage.
基金Supported by Oversea Project of National Natural and Science Foundationof China(30528005)RTOP Project of International RiceResearch Institute(IRRI)+1 种基金Grain High-yield Project of China(2004BA520A12)And 948 Introduction Project of the Ministry ofAgriculture(2003-Z53)~~
文摘Objective The aim was to elucidate the effects of N rates on rice canopy microclimate and community health so as to provide a sci- entific basis for studying the production potential in irrigated rice with healthy canopy. Method The effects of rice population structure traits under different N rates on rice canopy temperature, relative humidity （ RH）, light transmittance and sheath blight were studied by using Sunscan canopy analysis system and HOBO（ Pro Temp/RH IS logger）. Result The results showed that leaf area index, plant height and tiller number had significant effects on canopy cooling, RH enhancing and light reducing. Extremely significant multiple linear regression relationships existed among canopy day temperature, day RH, LAI and tiller number, and among light transmittance, tiller number and plant height. At flowering stage, per unit LAI could result in a day-maximum-temperature （Tmax） deceasing of 0.87℃ and a day-minimum-RH （RHmin） enhancing of 2.5% within canopy. Similarly, 100 plants per ms could respectively cause a Tmax deceasing of 1.23℃ and an RHmin enhancing of 3.3% in rice canopy. And per 10 cm plant height and 100 plants per m^2 could respectively reduce 9.3% and 7.8% of light in canopy. Conclusion Sheath blight disease index was significantly enhanced as the canopy day temperature decreased, day RH increased and light transmittance reduced. Bigger canopy from higher nitrogen level treatment leads to a more stable canopy microclimate with little changes in temperature and RH during day and night, which has the risk of worsening canopy health. Thus, moderately controlling the space development of canopy is the basis of constructing healthy canopy in rice.
