Stability of Defibrase in various pH buffer solutions was investigated. Enzyme-linked immuno-sorbent assay (ELISA) and coagulating time method were used to assess antigenic stability and coagulating stability, respect...Stability of Defibrase in various pH buffer solutions was investigated. Enzyme-linked immuno-sorbent assay (ELISA) and coagulating time method were used to assess antigenic stability and coagulating stability, respectively. The change of antigenic activities and coagulating activities of Defibrase in the same buffer solutions (pH 6, 7 and 8, with the exception of pH 3.6) showed similar tendency to decline with the time. Concentrated Defi-brase was relatively stable at neutral pH 6~7, more than 95% of its initial activities (100BUmL-1) was kept after a 10-day storage at 40 oC, whereas in pH 3.6 and pH 9 buffer solutions, diluted Defibrase was very labile. Addition of Triton X-100 or bovine serum albumin could effectively prevent loss of Defibrase by minimizing adsorption of De-fibrase to plastic surface (P<0.005). Concentration of Defibrase could also affect its stability in aqueous solutions.展开更多
使用全自动基因分析仪（ABI-3700 DNA Analyzer），用35对SSR荧光标记引物对来自61个国家的2625份大麦种质资源的遗传多样性进行了分析，得出如下结果：（1）35对引物在2625份大麦种质资源中共检测到2063个等位变异，每个位点的等位变...使用全自动基因分析仪（ABI-3700 DNA Analyzer），用35对SSR荧光标记引物对来自61个国家的2625份大麦种质资源的遗传多样性进行了分析，得出如下结果：（1）35对引物在2625份大麦种质资源中共检测到2063个等位变异，每个位点的等位变异数为30～79个，平均为58.94；多样性指数为1.60～3.66，平均为2.80；（2）在大麦的7条染色体中，每条染色体的等位变异数不等，染色体等位变异数从多到少排序为5、7、2、6、3、4、1，遗传多样性指数从高到低依次为2、6、7、3、5、4、1，综合这两项指标，第2、7条染色体遗传多样性较高，第1条染色体的遗传多样性最低；（3）约旦、伊朗、叙利亚和利比亚等国家大麦的遗传多样性指数最高，分别为2.58、2.53、2.50和2.38；而埃塞俄比亚、叙利亚、伊朗和约旦等国家的大麦资源则具有较多的等位变异，分别为29.03、25.57、25.11和24.60，综合分析，约旦、伊朗、叙利亚和土耳其大麦遗传多样性较高。展开更多
Insulin-loaded poly(lactide-co-glycolide) nanoparticles (INS-PLGA-NPs) were prepared by a double emulsion method (w/o/w), using ethyl acetate as organic solvent and poloxamer188 as emulsifier. Experimental parameter...Insulin-loaded poly(lactide-co-glycolide) nanoparticles (INS-PLGA-NPs) were prepared by a double emulsion method (w/o/w), using ethyl acetate as organic solvent and poloxamer188 as emulsifier. Experimental parameters such as the emulsifier and PLGA concentrations, the pH and concentration of the insulin solution, the solvent evaporation method and PVA in the internal phase were investigated for the encapsulation efficiency. The results indicated that higher emulsifier concentration, relatively less amount of PLGA and lower insulin concentration would increase the entrapment of insulin. Furthermore, pH of insulin solution approaching to pI (5.3), adding some PVA to the internal phase and a shorter evaporation time helped to enhance the incorporation efficiency of insulin. Optimized preparation parameters led to nanoparticles with well-defined characteristics such as an average size around 149.6 nm, a polydispersity lower than 0.1 and high encapsulation efficiency up to 42.8%.展开更多
文摘Stability of Defibrase in various pH buffer solutions was investigated. Enzyme-linked immuno-sorbent assay (ELISA) and coagulating time method were used to assess antigenic stability and coagulating stability, respectively. The change of antigenic activities and coagulating activities of Defibrase in the same buffer solutions (pH 6, 7 and 8, with the exception of pH 3.6) showed similar tendency to decline with the time. Concentrated Defi-brase was relatively stable at neutral pH 6~7, more than 95% of its initial activities (100BUmL-1) was kept after a 10-day storage at 40 oC, whereas in pH 3.6 and pH 9 buffer solutions, diluted Defibrase was very labile. Addition of Triton X-100 or bovine serum albumin could effectively prevent loss of Defibrase by minimizing adsorption of De-fibrase to plastic surface (P<0.005). Concentration of Defibrase could also affect its stability in aqueous solutions.
文摘使用全自动基因分析仪（ABI-3700 DNA Analyzer），用35对SSR荧光标记引物对来自61个国家的2625份大麦种质资源的遗传多样性进行了分析，得出如下结果：（1）35对引物在2625份大麦种质资源中共检测到2063个等位变异，每个位点的等位变异数为30～79个，平均为58.94；多样性指数为1.60～3.66，平均为2.80；（2）在大麦的7条染色体中，每条染色体的等位变异数不等，染色体等位变异数从多到少排序为5、7、2、6、3、4、1，遗传多样性指数从高到低依次为2、6、7、3、5、4、1，综合这两项指标，第2、7条染色体遗传多样性较高，第1条染色体的遗传多样性最低；（3）约旦、伊朗、叙利亚和利比亚等国家大麦的遗传多样性指数最高，分别为2.58、2.53、2.50和2.38；而埃塞俄比亚、叙利亚、伊朗和约旦等国家的大麦资源则具有较多的等位变异，分别为29.03、25.57、25.11和24.60，综合分析，约旦、伊朗、叙利亚和土耳其大麦遗传多样性较高。
文摘Insulin-loaded poly(lactide-co-glycolide) nanoparticles (INS-PLGA-NPs) were prepared by a double emulsion method (w/o/w), using ethyl acetate as organic solvent and poloxamer188 as emulsifier. Experimental parameters such as the emulsifier and PLGA concentrations, the pH and concentration of the insulin solution, the solvent evaporation method and PVA in the internal phase were investigated for the encapsulation efficiency. The results indicated that higher emulsifier concentration, relatively less amount of PLGA and lower insulin concentration would increase the entrapment of insulin. Furthermore, pH of insulin solution approaching to pI (5.3), adding some PVA to the internal phase and a shorter evaporation time helped to enhance the incorporation efficiency of insulin. Optimized preparation parameters led to nanoparticles with well-defined characteristics such as an average size around 149.6 nm, a polydispersity lower than 0.1 and high encapsulation efficiency up to 42.8%.
