Japanese scallop is one of the most important cultured seashells in the world. Following about 20 years’ artificial culture, we need DNA markers for genetic research. Microsatellites recently have become popular mark...Japanese scallop is one of the most important cultured seashells in the world. Following about 20 years’ artificial culture, we need DNA markers for genetic research. Microsatellites recently have become popular markers in a variety of genetic investigations. In this research, we isolated microsatellite DNAs from the Japanese scallop Patinopecten yessoensis genome with magnetic beads. As a result, 136 positive clones were identified from 192 clones,and 179 microsatellites were found. Among the 179 microsatellites, 50.8% were perfect, 43.0% were imperfect and the rest were compound type (6.1%). Finally, 85 microsatellite primers were designed by software Primer Premier 5.0, and 40 pairs were screened; 15 loci showed polymorphism. In these microsatellite loci, genetic diversity was analysed in 2 cultured populations. The average number of allele (A) ranged from 3.27 to 3.40; the average heterozygosity (H_o) in 2 populations was 0.447 to 0.540; average excepted heterozygosity (H_e) was 0.468 to 0.493; and average polymorphism information content (PIC) was 0.443 to 0.463. The genetic distance was 0.1155 and genetic identity was 0.8909. Thirteen microsatellite loci except HLJX-028 and HJLX-031 could be used for genetic展开更多
文摘Japanese scallop is one of the most important cultured seashells in the world. Following about 20 years’ artificial culture, we need DNA markers for genetic research. Microsatellites recently have become popular markers in a variety of genetic investigations. In this research, we isolated microsatellite DNAs from the Japanese scallop Patinopecten yessoensis genome with magnetic beads. As a result, 136 positive clones were identified from 192 clones,and 179 microsatellites were found. Among the 179 microsatellites, 50.8% were perfect, 43.0% were imperfect and the rest were compound type (6.1%). Finally, 85 microsatellite primers were designed by software Primer Premier 5.0, and 40 pairs were screened; 15 loci showed polymorphism. In these microsatellite loci, genetic diversity was analysed in 2 cultured populations. The average number of allele (A) ranged from 3.27 to 3.40; the average heterozygosity (H_o) in 2 populations was 0.447 to 0.540; average excepted heterozygosity (H_e) was 0.468 to 0.493; and average polymorphism information content (PIC) was 0.443 to 0.463. The genetic distance was 0.1155 and genetic identity was 0.8909. Thirteen microsatellite loci except HLJX-028 and HJLX-031 could be used for genetic
文摘抗中性粒细胞胞质抗体(anti-neutrophil cytoplasmic antibodies,ANCA)相关性血管炎(ANCA-associated vasculitis,AAV)是一种少见的以小血管受累为主的系统性自身免疫性疾病。主要包括显微镜下多血管炎(microscopic polyangiitis,MPA),肉芽肿性多血管炎(granulomatosis with polyangiitis,GPA)[原称为韦格纳肉芽肿(Wegener s granulomatosis,WG)]和嗜酸性肉芽肿性多血管炎(eosinophilic granulomatosis with polyangiitis,EGPA)(原称为Churg-Strauss综合征)3种类型[1-2]。AAV可发生于任何年龄段,在欧洲其患病率为每年每百万人20~25例。AAV可影响全身各个部位,最常见的是上呼吸道、肺脏、肾脏、眼以及周围神经[3],中枢神经系统受累并不多见(<15%)[4]。由于中枢神经系统症状的复杂多变,影响了AAV患者的早期诊断,延误治疗,造成患者的不良预后、疾病复发甚至是死亡[4]。本研究总结了首都医科大学附属北京友谊医院近年来AAV合并出现神经系统症状和以神经系统症状为首发表现的14例AAV患者,通过对其临床表现、实验室检查和影像学检查资料的分析,提高临床医生对AAV的认知,做到早诊断早治疗。
