肾癌是肾实质泌尿小管上皮系统的一种恶性肿瘤,近年来发病率逐年增高,并呈年轻化趋势,是泌尿系统最常见的恶性肿瘤之一[1]。开放肾癌根治术是治疗肾癌常用的手术方式,随着微创技术的发展,腹腔镜肾癌根治术因创伤小、出血少、恢复快等优...肾癌是肾实质泌尿小管上皮系统的一种恶性肿瘤,近年来发病率逐年增高,并呈年轻化趋势,是泌尿系统最常见的恶性肿瘤之一[1]。开放肾癌根治术是治疗肾癌常用的手术方式,随着微创技术的发展,腹腔镜肾癌根治术因创伤小、出血少、恢复快等优势,逐渐用于临床[2]。随着进一步深入研究,有学者发现年龄、手术创伤应激、留置引流管等因素会影响患者术后快速康复[3]。术后强化康复(enhanced recovery after surgery,ERAS)是在围手术期采取一系列具有循证医学依据的优化管理举措,可减轻患者生理、心理的创伤和应激反应,帮助患者快速恢复。已有研究显示,ERAS理念目前已广泛用于骨科、妇科、胃肠外科等临床科室,可减少术后短期并发症,缩短住院时间,在临床上取得了满意的疗效,同时,其在泌尿外科围手术期中的应用也逐渐得到广大泌尿外科专家的认可[4-5]。本研究将进一步探讨ERAS理念应用于腹腔镜肾癌根治术围手术期患者中的效果,现报道如下。展开更多
Emerging evidence has indicated that circular RNAs（circRNAs） play pivotal roles in the regulation of cellular processes and are found to be aberrantly expressed in a variety of tumors. However, the clinical role of ...Emerging evidence has indicated that circular RNAs（circRNAs） play pivotal roles in the regulation of cellular processes and are found to be aberrantly expressed in a variety of tumors. However, the clinical role of circ RNAs in bladder cancer（BC） and the molecular mechanisms have yet to be fully understood. In this study, the clinical specimens were obtained and the expression level of a circ RNA BCRC4 was detected by real-time PCR in both BC tissues and cell line. The circular RNA over-expression plasmid was constructed and transfected into BC cells and related cell line. The cell cycles and apoptosis were observed using inverted microscope and flow cytometry. Western blotting was used to compare the relative protein expression of groups with different treatments. It was found that circ RNA BCRC4 expression was lower in BC tissues than in adjacent normal tissues. Furthermore, consequences of forced-expression of BCRC4 promoted apoptosis and inhibited viability of T24T and UMUC3 cells, and up-regulated BCRC4-increased miR-101 level, which suppressed EZH2 expression in both RNA and protein levels. In addition, gambogic acid（GA） is a promising natural anticancer compound for BC therapy, and GA treatment increased the BCRC4 expression in T24T and UMUC3 cells in a dose-dependent manner. Altogether, our findings suggest that BCRC4 functions as a tumor suppressor in BC, and mediates anticancer function, at least in part, by up-regulating the expression of miR-101. Targeting this newly identified circ RNA may help us develop a novel strategy for treating human BC.展开更多
文摘肾癌是肾实质泌尿小管上皮系统的一种恶性肿瘤,近年来发病率逐年增高,并呈年轻化趋势,是泌尿系统最常见的恶性肿瘤之一[1]。开放肾癌根治术是治疗肾癌常用的手术方式,随着微创技术的发展,腹腔镜肾癌根治术因创伤小、出血少、恢复快等优势,逐渐用于临床[2]。随着进一步深入研究,有学者发现年龄、手术创伤应激、留置引流管等因素会影响患者术后快速康复[3]。术后强化康复(enhanced recovery after surgery,ERAS)是在围手术期采取一系列具有循证医学依据的优化管理举措,可减轻患者生理、心理的创伤和应激反应,帮助患者快速恢复。已有研究显示,ERAS理念目前已广泛用于骨科、妇科、胃肠外科等临床科室,可减少术后短期并发症,缩短住院时间,在临床上取得了满意的疗效,同时,其在泌尿外科围手术期中的应用也逐渐得到广大泌尿外科专家的认可[4-5]。本研究将进一步探讨ERAS理念应用于腹腔镜肾癌根治术围手术期患者中的效果,现报道如下。
文摘Emerging evidence has indicated that circular RNAs（circRNAs） play pivotal roles in the regulation of cellular processes and are found to be aberrantly expressed in a variety of tumors. However, the clinical role of circ RNAs in bladder cancer（BC） and the molecular mechanisms have yet to be fully understood. In this study, the clinical specimens were obtained and the expression level of a circ RNA BCRC4 was detected by real-time PCR in both BC tissues and cell line. The circular RNA over-expression plasmid was constructed and transfected into BC cells and related cell line. The cell cycles and apoptosis were observed using inverted microscope and flow cytometry. Western blotting was used to compare the relative protein expression of groups with different treatments. It was found that circ RNA BCRC4 expression was lower in BC tissues than in adjacent normal tissues. Furthermore, consequences of forced-expression of BCRC4 promoted apoptosis and inhibited viability of T24T and UMUC3 cells, and up-regulated BCRC4-increased miR-101 level, which suppressed EZH2 expression in both RNA and protein levels. In addition, gambogic acid（GA） is a promising natural anticancer compound for BC therapy, and GA treatment increased the BCRC4 expression in T24T and UMUC3 cells in a dose-dependent manner. Altogether, our findings suggest that BCRC4 functions as a tumor suppressor in BC, and mediates anticancer function, at least in part, by up-regulating the expression of miR-101. Targeting this newly identified circ RNA may help us develop a novel strategy for treating human BC.
