Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However,...Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However, to obtain sufficient RNA from laser-capture microdissected cells is quite difficult. The study was designed to determinc a feasible technical routine to isolate transitional cells from bladder membrane, separate carcinoma cclls from stromal cells and to amplify the RNA isolated from laser-capture microdissected cells. Methods： Bladder transitional cell were obtained from frozen sections of bladder membrane applying LCM, by the same token, BTCC cells from frozen sections of BTCC tissue. Then RNA was extracted and linearly amplified in vitro. The expression levels of β-actin in primary total RNA and amplified RNA were detected using RT-PCR. Results： That RNA integrity was good after LCM was confirmed by control experiment Ⅰ; By control experiment Ⅱ, the correlation between the number of LCM-shooting and RNA quantity undcr arranged conditions was preliminarily confirmed. About 0.5-2.5kb RNA fragments were obtained after RNA amplification and β-actin levels were integral. Conclusion： Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.展开更多
目的:探讨原发性女性生殖系统非霍奇金淋巴瘤(primary female genital system lymphoma,PFGSL)的临床特征、治疗及预后。方法:回顾性分析天津医科大学附属肿瘤医院1976年1月～2006年8月收治的25例原发性女性生殖系统非霍奇金淋巴瘤患者...目的:探讨原发性女性生殖系统非霍奇金淋巴瘤(primary female genital system lymphoma,PFGSL)的临床特征、治疗及预后。方法:回顾性分析天津医科大学附属肿瘤医院1976年1月～2006年8月收治的25例原发性女性生殖系统非霍奇金淋巴瘤患者的临床资料,分析其临床分期、恶性程度、治疗及影响预后的因素。结果:25例PFGSL患者平均年龄41.2岁,病变部位分别位于卵巢(11例)、宫颈(7例)、子宫(5例)和外阴(2例)。其中B细胞来源22例,T细胞来源1例,来源不明2例。按照国际工作分类(IWF),PFGSL低、中度恶性占78.3%,高度恶性占21.7%。按AnnArbor分期,ⅠE期8例(32.0%),ⅡE和ⅣE期各6例(24.0%),ⅢE期5例(20%)。治疗方法采用手术和化疗为主的综合治疗,采用手术+放疗+化疗3例,手术+化疗18例,放疗+化疗2例,单纯手术2例。全组患者中位生存期(MST)25个月,5年生存率32.0%。研究发现原发部位、临床分期及IWF不同,其生存期差异有显著性。原发于卵巢者预后最差,晚期(ⅢE,ⅣE)、高度恶性的病例预后明显差于早期(IE,ⅡE)、低度恶性的病例。结论:PFGSL临床症状不典型,预后较差,治疗方法应采用手术及化疗为主的综合治疗;原发部位、临床分期及IWF是预后的影响因素。展开更多
文摘Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However, to obtain sufficient RNA from laser-capture microdissected cells is quite difficult. The study was designed to determinc a feasible technical routine to isolate transitional cells from bladder membrane, separate carcinoma cclls from stromal cells and to amplify the RNA isolated from laser-capture microdissected cells. Methods： Bladder transitional cell were obtained from frozen sections of bladder membrane applying LCM, by the same token, BTCC cells from frozen sections of BTCC tissue. Then RNA was extracted and linearly amplified in vitro. The expression levels of β-actin in primary total RNA and amplified RNA were detected using RT-PCR. Results： That RNA integrity was good after LCM was confirmed by control experiment Ⅰ; By control experiment Ⅱ, the correlation between the number of LCM-shooting and RNA quantity undcr arranged conditions was preliminarily confirmed. About 0.5-2.5kb RNA fragments were obtained after RNA amplification and β-actin levels were integral. Conclusion： Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.
文摘目的:探讨原发性女性生殖系统非霍奇金淋巴瘤(primary female genital system lymphoma,PFGSL)的临床特征、治疗及预后。方法:回顾性分析天津医科大学附属肿瘤医院1976年1月～2006年8月收治的25例原发性女性生殖系统非霍奇金淋巴瘤患者的临床资料,分析其临床分期、恶性程度、治疗及影响预后的因素。结果:25例PFGSL患者平均年龄41.2岁,病变部位分别位于卵巢(11例)、宫颈(7例)、子宫(5例)和外阴(2例)。其中B细胞来源22例,T细胞来源1例,来源不明2例。按照国际工作分类(IWF),PFGSL低、中度恶性占78.3%,高度恶性占21.7%。按AnnArbor分期,ⅠE期8例(32.0%),ⅡE和ⅣE期各6例(24.0%),ⅢE期5例(20%)。治疗方法采用手术和化疗为主的综合治疗,采用手术+放疗+化疗3例,手术+化疗18例,放疗+化疗2例,单纯手术2例。全组患者中位生存期(MST)25个月,5年生存率32.0%。研究发现原发部位、临床分期及IWF不同,其生存期差异有显著性。原发于卵巢者预后最差,晚期(ⅢE,ⅣE)、高度恶性的病例预后明显差于早期(IE,ⅡE)、低度恶性的病例。结论:PFGSL临床症状不典型,预后较差,治疗方法应采用手术及化疗为主的综合治疗;原发部位、临床分期及IWF是预后的影响因素。
