目的:建立不同程度的大鼠神经病理性疼痛模型,评价其行为学和病理学变化的关系。方法:30只雄性SD大鼠随机均分为C,N0,N1,N2,N4组(n=6),用分级坐骨神经缩窄法建立动物模型。在术前和术后3、7、10、14天测定大鼠右后爪50%缩爪阈值。术后...目的:建立不同程度的大鼠神经病理性疼痛模型,评价其行为学和病理学变化的关系。方法:30只雄性SD大鼠随机均分为C,N0,N1,N2,N4组(n=6),用分级坐骨神经缩窄法建立动物模型。在术前和术后3、7、10、14天测定大鼠右后爪50%缩爪阈值。术后第15天取右侧坐骨神经横断面切片行Luxol fast blue染色,计算坐骨神经环扎远端髓鞘计数占近端髓鞘计数百分比(the ratiosof the myelin sheath counts in the distal sections to those in the proximal sections of the sciatic nerve,DPR)。用SPSS 13.0进行数据分析。结果:大鼠右后爪50%缩爪阈值在C组和N0组无明显改变,在N1、N2和N4组大鼠术后较术前明显下降,至术后14天N0>N1>N2>N4(P<0.05)。C组和N0组大鼠右侧坐骨神经无明显病理学改变,N1、N2和N4组环扎远端神经纤维出现不同程度的脱髓鞘改变。术后14天大鼠50%缩爪阈值与其DPR值间Spearman相关系数rp=0.901(P<0.001)。结论:大鼠分级坐骨神经缩窄模型可导致手术侧坐骨神经不同程度的脱髓鞘病变,引起不同程度的神经病理性疼痛,其病理学变化与行为学有显著相关性。展开更多
This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current （INMDA） and the expression of cytochrome C in cultured developing rat hippocampal neurons...This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current （INMDA） and the expression of cytochrome C in cultured developing rat hippocampal neurons. The hippocampi were dissected from newborn Sprague-Dawley rats. Hippocampal neurons were primarily cultured for 5 days and then treated with different concentrations of isoflurane [（0.25, 0.5, 0.75, 1 minimum alveolar concentration （MAC））]. The peak of INMDA was re- corded by means of the whole cell patch clamp technique. The cytochrome C level was detected by Western blotting and quantitative real-time PCR. Our results showed that isoflurane （0.25, 0.5, 0.75 and 1 MAC） potentiated the amplitude of INMDA by （116±8.8）%, （122±11.7）%, （135±14.3）% and （132~14.6）%, respectively, and isoflurane increased the mRNA expression of cytochrome C in a concentration-dependent manner. The cytochrome C mRNA expression reached a maximum after 0.5 MAC isoflurane stimulation for 6 h （P〈0.05）. It was concluded that isoflurane enhances the expression of cytochrome C in cultured rat hippocampal neurons, which may be mediated by facilitation of NMDA receptor.展开更多
文摘目的:建立不同程度的大鼠神经病理性疼痛模型,评价其行为学和病理学变化的关系。方法:30只雄性SD大鼠随机均分为C,N0,N1,N2,N4组(n=6),用分级坐骨神经缩窄法建立动物模型。在术前和术后3、7、10、14天测定大鼠右后爪50%缩爪阈值。术后第15天取右侧坐骨神经横断面切片行Luxol fast blue染色,计算坐骨神经环扎远端髓鞘计数占近端髓鞘计数百分比(the ratiosof the myelin sheath counts in the distal sections to those in the proximal sections of the sciatic nerve,DPR)。用SPSS 13.0进行数据分析。结果:大鼠右后爪50%缩爪阈值在C组和N0组无明显改变,在N1、N2和N4组大鼠术后较术前明显下降,至术后14天N0>N1>N2>N4(P<0.05)。C组和N0组大鼠右侧坐骨神经无明显病理学改变,N1、N2和N4组环扎远端神经纤维出现不同程度的脱髓鞘改变。术后14天大鼠50%缩爪阈值与其DPR值间Spearman相关系数rp=0.901(P<0.001)。结论:大鼠分级坐骨神经缩窄模型可导致手术侧坐骨神经不同程度的脱髓鞘病变,引起不同程度的神经病理性疼痛,其病理学变化与行为学有显著相关性。
基金supported by grants from the National Natural Science Foundation of China(No.30772086No.30901390)
文摘This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current （INMDA） and the expression of cytochrome C in cultured developing rat hippocampal neurons. The hippocampi were dissected from newborn Sprague-Dawley rats. Hippocampal neurons were primarily cultured for 5 days and then treated with different concentrations of isoflurane [（0.25, 0.5, 0.75, 1 minimum alveolar concentration （MAC））]. The peak of INMDA was re- corded by means of the whole cell patch clamp technique. The cytochrome C level was detected by Western blotting and quantitative real-time PCR. Our results showed that isoflurane （0.25, 0.5, 0.75 and 1 MAC） potentiated the amplitude of INMDA by （116±8.8）%, （122±11.7）%, （135±14.3）% and （132~14.6）%, respectively, and isoflurane increased the mRNA expression of cytochrome C in a concentration-dependent manner. The cytochrome C mRNA expression reached a maximum after 0.5 MAC isoflurane stimulation for 6 h （P〈0.05）. It was concluded that isoflurane enhances the expression of cytochrome C in cultured rat hippocampal neurons, which may be mediated by facilitation of NMDA receptor.
