建立液相色谱—串联质谱法(LC-MS/MS)检测乙氧磺隆和双环磺草酮在水稻中残留分析方法。样品经乙腈溶液涡旋提取,NaCl盐析,0.25 g MgSO4、0.05 g PSA和0.05 g C18净化后,采用电喷雾离子源正离子扫描,多反应监测(MRM)模式下,外标法定量。...建立液相色谱—串联质谱法(LC-MS/MS)检测乙氧磺隆和双环磺草酮在水稻中残留分析方法。样品经乙腈溶液涡旋提取,NaCl盐析,0.25 g MgSO4、0.05 g PSA和0.05 g C18净化后,采用电喷雾离子源正离子扫描,多反应监测(MRM)模式下,外标法定量。结果表明:在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。当添加浓度为0.01~1.0 mg/kg时,在糙米中的平均回收率为84%~107%,相对标准偏差为1%~8%。当添加浓度为0.05~2.5 mg/kg时,在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。在稻壳中的平均回收率为86%~101%,相对标准偏差为2%~8%;在秸秆中的平均回收率为87%~99%,相对标准偏差为6%~11%。乙氧磺隆和双环磺草酮在糙米中的定量限为0.01 mg/kg,在稻壳和秸秆中的定量限为0.05 mg/kg。展开更多
[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, co...[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, cocklebur （grain）, basil （leaves）, ragweed （stems and leaves）, green onion （root）, garlic （tuber）, Yuzhu （leaves）, marshpeper smartweed （whole plant）, horseweed （aboveground stems and leaves） were dried, grinded and sieved. The powders were then extracted by ethanol at room temperature for 24 h. The extract solutions were filtered through fil- ter pater. The obtained plant extracts were then diluted to determine their nematici- dal activities. [Result] The Chuanxiong extract had the highest nematicidal activity, followed by cocklebur extract, agrimony extract, basil extract, garlic extract, ragweed extract. The horseweed extract had the lowest nematicidal activity. [Conclusion] The Chuanxiong and other plants were natural, and they had a good environmental compatibility. They could be developed as botanical pesticides against Hirschman- niella spp.展开更多
[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse condition...[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse conditions.[Result] The reduction of more than 80% in fresh weight was observed in S.pygmaea treated with 45 g/hm2 of pyrazosulfuron-ethyl for 7 d or more.Application of 60 g/hm2 at the sprouting stage of S.pygmaea suppressed completely the growth in fresh weight irrespective of tuber size.However,at 6 g/hm2 growth of the weed sustained less reduction with increasing tuber size.Differences in flooding water depth and temperature had no appreciable influence on the herbicidal activity.Growth inhibition of S.pygmaea by pre-emergence or 1.5-leaf stage applications under non-leaching conditions was significantly higher than that under water leaching conditions.Under non-leaching condition,there was no remarkable difference in this inhibition between preemergence and 1.5-leaf stage applications,while under water leaching conditions the growth reduction with the latter application was significantly larger.Growth inhibition of S.pygmaea planted at a depth of 2 cm was significantly higher than that planted deeper （5 cm） with the pre-emergence applications.[Conclusion] This study had provide basis for the further study on the inhibition effect of pyrazosulfuron-ethyl.展开更多
文摘建立液相色谱—串联质谱法(LC-MS/MS)检测乙氧磺隆和双环磺草酮在水稻中残留分析方法。样品经乙腈溶液涡旋提取,NaCl盐析,0.25 g MgSO4、0.05 g PSA和0.05 g C18净化后,采用电喷雾离子源正离子扫描,多反应监测(MRM)模式下,外标法定量。结果表明:在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。当添加浓度为0.01~1.0 mg/kg时,在糙米中的平均回收率为84%~107%,相对标准偏差为1%~8%。当添加浓度为0.05~2.5 mg/kg时,在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。在稻壳中的平均回收率为86%~101%,相对标准偏差为2%~8%;在秸秆中的平均回收率为87%~99%,相对标准偏差为6%~11%。乙氧磺隆和双环磺草酮在糙米中的定量限为0.01 mg/kg,在稻壳和秸秆中的定量限为0.05 mg/kg。
基金Supported by Science and Technology Project of Hunan Provincial Department of Education(11A059)Earmarked Fund for China Agriculture Research System(CARS19-05B)~~
文摘[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, cocklebur （grain）, basil （leaves）, ragweed （stems and leaves）, green onion （root）, garlic （tuber）, Yuzhu （leaves）, marshpeper smartweed （whole plant）, horseweed （aboveground stems and leaves） were dried, grinded and sieved. The powders were then extracted by ethanol at room temperature for 24 h. The extract solutions were filtered through fil- ter pater. The obtained plant extracts were then diluted to determine their nematici- dal activities. [Result] The Chuanxiong extract had the highest nematicidal activity, followed by cocklebur extract, agrimony extract, basil extract, garlic extract, ragweed extract. The horseweed extract had the lowest nematicidal activity. [Conclusion] The Chuanxiong and other plants were natural, and they had a good environmental compatibility. They could be developed as botanical pesticides against Hirschman- niella spp.
基金Supported by the Science and Technology Innovation Platform Construction Project of Institution of Higher Education in Hunan Province(XCJZ2009-070)the Earmarked Fund for Mordern Agro-industry Technology Research System(CARS-19-E12)~~
文摘[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse conditions.[Result] The reduction of more than 80% in fresh weight was observed in S.pygmaea treated with 45 g/hm2 of pyrazosulfuron-ethyl for 7 d or more.Application of 60 g/hm2 at the sprouting stage of S.pygmaea suppressed completely the growth in fresh weight irrespective of tuber size.However,at 6 g/hm2 growth of the weed sustained less reduction with increasing tuber size.Differences in flooding water depth and temperature had no appreciable influence on the herbicidal activity.Growth inhibition of S.pygmaea by pre-emergence or 1.5-leaf stage applications under non-leaching conditions was significantly higher than that under water leaching conditions.Under non-leaching condition,there was no remarkable difference in this inhibition between preemergence and 1.5-leaf stage applications,while under water leaching conditions the growth reduction with the latter application was significantly larger.Growth inhibition of S.pygmaea planted at a depth of 2 cm was significantly higher than that planted deeper （5 cm） with the pre-emergence applications.[Conclusion] This study had provide basis for the further study on the inhibition effect of pyrazosulfuron-ethyl.
文摘采用磷酸缓冲液浸提法提取玉竹糖蛋白粗品(Polygonatum odoratum glycoprotein,PDG),经葡聚糖凝胶G-100和刀豆凝集素A分离纯化,得到糖蛋白组分PDG1和PDG3,经高效液相色谱测定其分子质量,并检测PDG、PDG1和PDG3的体外抗氧化活性。结果表明,PDG1和PDG3的分子质量分别为186 k D和28.3 k D;玉竹糖蛋白具有一定的还原能力,对羟自由基和1,1-二苯基-2-三硝基苯肼自由基具有较强的清除作用,对脂质过氧化具有抑制作用,且在一定质量浓度范围内(1~10 mg/m L)存在剂量关系,其抗氧化能力随着玉竹糖蛋白质量浓度的增加而增强,PDG1和PDG3的抗氧化能力优于PDG。
