A maize (Zea mays L.) genome_specific repeated DNA sequence (clone MR64) has been transferred into one DH line of wheat through wheat (Triticum persicum Vav. ex Zhuk.) and maize cross. In the present study by RFLP ana...A maize (Zea mays L.) genome_specific repeated DNA sequence (clone MR64) has been transferred into one DH line of wheat through wheat (Triticum persicum Vav. ex Zhuk.) and maize cross. In the present study by RFLP analysis the authors proved that this DNA sequence could stably transmit into DH3 plants, the next generation derived from DH2 self_crossing. A similarity search in all DNA databases using BLASTN program showed that the DNA sequence of MR64 had as high as 93% identity to PREM_2 and 79% to Opie_2 in nucleotides. Both PREM_2 and Opie_2 are known as retrotransposons in maize genome, suggesting that MR64 likely is the partial sequence of a maize retrotransposon. Therefore, the results indicate that some retrotransposon might involve the DNA introgression from maize to wheat genome through wide fertilization. Stable inheritance of this maize genome_specific retrotransposon_like DNA in the wheat genome opens up the possibility of using retrotransposon as a new tool for gene tagging, function analysis, and insertional mutagenesis in wheat genome.展开更多
Nearly 100 maize specific repeated DNA clones were screened from a maize random genomic library, and used for RFLP analysis of two wheat DH populations from wheat and maize crosses. The result showed that a maize frag...Nearly 100 maize specific repeated DNA clones were screened from a maize random genomic library, and used for RFLP analysis of two wheat DH populations from wheat and maize crosses. The result showed that a maize fragment in clone MR64 was transferred into two wheat DH lines, i.e. No.18 in common wheat DHs and No.15 in persicum wheat DHs, in which strong hybridization signals were detected. It is first proved at DNA level that some maize specific DNA can be transferred into wheat DH progenies through fertilization at low frequency. Sequencing revealed that the size of the cloned insert DNA is 695 bp, containing 58% A+T. Southern hybridization to maize genome with several restriction enzymes indicated that MR64 is a dispersed repeat with 2-3 dominant tandem units. Its sequence structure, methylation pattern, copy number and chromosomal distribution were also determined. The possible mechanism of alien DNA transfer was discussed.展开更多
文摘A maize (Zea mays L.) genome_specific repeated DNA sequence (clone MR64) has been transferred into one DH line of wheat through wheat (Triticum persicum Vav. ex Zhuk.) and maize cross. In the present study by RFLP analysis the authors proved that this DNA sequence could stably transmit into DH3 plants, the next generation derived from DH2 self_crossing. A similarity search in all DNA databases using BLASTN program showed that the DNA sequence of MR64 had as high as 93% identity to PREM_2 and 79% to Opie_2 in nucleotides. Both PREM_2 and Opie_2 are known as retrotransposons in maize genome, suggesting that MR64 likely is the partial sequence of a maize retrotransposon. Therefore, the results indicate that some retrotransposon might involve the DNA introgression from maize to wheat genome through wide fertilization. Stable inheritance of this maize genome_specific retrotransposon_like DNA in the wheat genome opens up the possibility of using retrotransposon as a new tool for gene tagging, function analysis, and insertional mutagenesis in wheat genome.
文摘Nearly 100 maize specific repeated DNA clones were screened from a maize random genomic library, and used for RFLP analysis of two wheat DH populations from wheat and maize crosses. The result showed that a maize fragment in clone MR64 was transferred into two wheat DH lines, i.e. No.18 in common wheat DHs and No.15 in persicum wheat DHs, in which strong hybridization signals were detected. It is first proved at DNA level that some maize specific DNA can be transferred into wheat DH progenies through fertilization at low frequency. Sequencing revealed that the size of the cloned insert DNA is 695 bp, containing 58% A+T. Southern hybridization to maize genome with several restriction enzymes indicated that MR64 is a dispersed repeat with 2-3 dominant tandem units. Its sequence structure, methylation pattern, copy number and chromosomal distribution were also determined. The possible mechanism of alien DNA transfer was discussed.
