Research and application of bioreactor for organic contaminated soil are reviewed in this paper,including technology and facilities of soil slurry reactor,immobilized soil reactor,rotary drum reactor,bio-fluidized bed...Research and application of bioreactor for organic contaminated soil are reviewed in this paper,including technology and facilities of soil slurry reactor,immobilized soil reactor,rotary drum reactor,bio-fluidized bed,and anaeration -aeration bioreactor.It is pointed out that the combination of physical,chemical methods with bioreactor technology will be of great interest.展开更多
以 Pseudomonas sn Gx。-1发酵培养得到的细菌胞外聚合物作吸附剂,研究其对水中重金属Cr(VI)的吸附。吸附剂对Cr(VI)吸附的最佳pH初值为05-20。Cr(VI)的吸附分三个阶段:srnin达乃％的快速吸附阶段;10-40min达表观一级反应动力学吸附阶段...以 Pseudomonas sn Gx。-1发酵培养得到的细菌胞外聚合物作吸附剂,研究其对水中重金属Cr(VI)的吸附。吸附剂对Cr(VI)吸附的最佳pH初值为05-20。Cr(VI)的吸附分三个阶段:srnin达乃％的快速吸附阶段;10-40min达表观一级反应动力学吸附阶段;50min以后趋于平衡。吸附过程符合Freundlich和Langmuir吸附等温方程。展开更多
目的探讨沉默信息调节因子3（SIRT3）对肝癌细胞增殖的影响。方法 Western blotting检测SIRT3在正常肝组织、永生化肝细胞及肝癌细胞系中的蛋白表达水平;在肝癌细胞中过表达SIRT3,台盼蓝排斥实验检测过表达SIRT3对肝癌细胞增殖的影响,E...目的探讨沉默信息调节因子3（SIRT3）对肝癌细胞增殖的影响。方法 Western blotting检测SIRT3在正常肝组织、永生化肝细胞及肝癌细胞系中的蛋白表达水平;在肝癌细胞中过表达SIRT3,台盼蓝排斥实验检测过表达SIRT3对肝癌细胞增殖的影响,Ed U标记实验检测SIRT3对肝癌细胞DNA合成的影响;在肝癌细胞中过表达SIRT3,平板集落实验检测SIRT3对肝癌细胞集落形成能力的影响;q RT-PCR验证SIRT3的沉默效率,台盼蓝排斥实验检测SIRT3沉默对肝癌细胞增殖的影响,Ed U标记实验检测SIRT3沉默对肝癌细胞DNA合成的影响。结果 SIRT3在肝癌细胞系中的蛋白水平较永生化肝细胞、正常肝组织降低;SIRT3在肝癌细胞中成功过表达,过表达SIRT3使肝癌细胞增殖数目减少了约为51%~61%（P〈0.001）,使肝癌细胞DNA合成下降了约57%（P〈0.05）;过表达SIRT3抑制肝癌细胞的集落形成能力;SIRT3沉默有效,SIRT3沉默使肝癌细胞的增殖增加了约51%~61%（P〈0.01）,使肝癌细胞的DNA合成能力增强了137%~149%（P〈0.01）。结论 SIRT3可抑制肝癌细胞的增殖。展开更多
文摘Research and application of bioreactor for organic contaminated soil are reviewed in this paper,including technology and facilities of soil slurry reactor,immobilized soil reactor,rotary drum reactor,bio-fluidized bed,and anaeration -aeration bioreactor.It is pointed out that the combination of physical,chemical methods with bioreactor technology will be of great interest.
文摘以 Pseudomonas sn Gx。-1发酵培养得到的细菌胞外聚合物作吸附剂,研究其对水中重金属Cr(VI)的吸附。吸附剂对Cr(VI)吸附的最佳pH初值为05-20。Cr(VI)的吸附分三个阶段:srnin达乃％的快速吸附阶段;10-40min达表观一级反应动力学吸附阶段;50min以后趋于平衡。吸附过程符合Freundlich和Langmuir吸附等温方程。
