Taking the tender leaves of Oplopanax elatus as explants for the experiment,Uniform Design were used to test the most suitable media for callus induction,differentiation of shoot and rooting.The results showed that N6...Taking the tender leaves of Oplopanax elatus as explants for the experiment,Uniform Design were used to test the most suitable media for callus induction,differentiation of shoot and rooting.The results showed that N6 + 6-BA 0.5 mg·L-1 + 2,4-D 0.2 mg·L-1+Vc 10 mg·L-1 was fitted for callus induction,the medium for differentiation of shoot was N6 + 6-BA 4.5 mg·L-1 +NAA 0.1 mg·L-1+Vc 20 mg·L-1,the medium for rooting was 1/4MS + IBA 0.1mg·L-1 +NAA 0.1 mg·L-1.Tissue culture required different kinds of culture media in different phases.展开更多
The tender buds of Phyllodoce caerulea were used as explants for this experiment.The most suitable culture media were screened for shoots regeneration directly from bases of the tender buds,rooting and germplasm prese...The tender buds of Phyllodoce caerulea were used as explants for this experiment.The most suitable culture media were screened for shoots regeneration directly from bases of the tender buds,rooting and germplasm preservation in vitro with a uniform design.The results showed that DR+TDZ4.00 mg·L-1was the most suitable for shoots regeneration,and the rate of regeneration was more than 98.5%.MS（modified）+IBA0.05 mg·L-1+NAA0.01 mg·L-1+KT0.10 mg·L-1was the most suitable for rooting,and the rate of rooting was more than 97.8%.N-68+B92.30 mg·L-1+ phloridzin 1.50 mg·L-1was the most suitable for preservation in vitro for 42 months.Stems each with one node were cut from the regenerated shoots and cultured for propagation,and a 35-fold proliferation rate was achieved within 40 days.The method of "deferring growth with dwarfing" was utilized for germplasm preservation in vitro at normal temperature.In vitro culture and germplasm preservation in vitro system of Phyllodoce caerulea had been successfully established.展开更多
文摘Taking the tender leaves of Oplopanax elatus as explants for the experiment,Uniform Design were used to test the most suitable media for callus induction,differentiation of shoot and rooting.The results showed that N6 + 6-BA 0.5 mg·L-1 + 2,4-D 0.2 mg·L-1+Vc 10 mg·L-1 was fitted for callus induction,the medium for differentiation of shoot was N6 + 6-BA 4.5 mg·L-1 +NAA 0.1 mg·L-1+Vc 20 mg·L-1,the medium for rooting was 1/4MS + IBA 0.1mg·L-1 +NAA 0.1 mg·L-1.Tissue culture required different kinds of culture media in different phases.
文摘The tender buds of Phyllodoce caerulea were used as explants for this experiment.The most suitable culture media were screened for shoots regeneration directly from bases of the tender buds,rooting and germplasm preservation in vitro with a uniform design.The results showed that DR+TDZ4.00 mg·L-1was the most suitable for shoots regeneration,and the rate of regeneration was more than 98.5%.MS（modified）+IBA0.05 mg·L-1+NAA0.01 mg·L-1+KT0.10 mg·L-1was the most suitable for rooting,and the rate of rooting was more than 97.8%.N-68+B92.30 mg·L-1+ phloridzin 1.50 mg·L-1was the most suitable for preservation in vitro for 42 months.Stems each with one node were cut from the regenerated shoots and cultured for propagation,and a 35-fold proliferation rate was achieved within 40 days.The method of "deferring growth with dwarfing" was utilized for germplasm preservation in vitro at normal temperature.In vitro culture and germplasm preservation in vitro system of Phyllodoce caerulea had been successfully established.
