以红颜草莓匍匐茎为外植体,采取热处理脱毒和微茎尖脱毒相结合、直接微茎尖脱毒两种脱毒方法,以次氯酸钠为消毒剂,探索不同脱毒方法、消毒时间、消毒浓度对无菌脱毒苗获得的影响,得出在5% NaClO,12 min处理后,直接进行微茎尖脱毒,成活...以红颜草莓匍匐茎为外植体,采取热处理脱毒和微茎尖脱毒相结合、直接微茎尖脱毒两种脱毒方法,以次氯酸钠为消毒剂,探索不同脱毒方法、消毒时间、消毒浓度对无菌脱毒苗获得的影响,得出在5% NaClO,12 min处理后,直接进行微茎尖脱毒,成活率能达到95%以上且长势最优,有明显生长情况。以MS为基本培养基,探索最佳增殖和生根培养基,增殖培养基为MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA时,增值系数为3.4。生根培养基为1/2 MS + 0.1 mg/L IBA时,生根率能达到100%,无菌脱毒苗移栽到草炭:蛭石:珍珠岩(1:1:1)的栽培基质中成活率为100%。本试验建立红颜草莓组培快繁体系,以期为草莓优良品种规模化繁育和工厂化生产提供技术支撑。Using the creeping stems of red strawberry as explants, two detoxification methods were adopted, including heat treatment detoxification and micro stem tip detoxification, and direct micro stem tip detoxification. Sodium hypochlorite was used as the disinfectant to explore the effects of different detoxification methods, disinfection time, and disinfection concentration on obtaining sterile and detoxified seedlings. It was found that after 12 minutes of treatment with 5% NaClO, direct micro stem tip detoxification resulted in a survival rate of over 95% and optimal growth, with significant growth. Using MS as the basic culture medium, explore the optimal proliferation and rooting culture medium. When the proliferation medium is MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA, the value-added coefficient is 3.4. When the rooting medium is 1/2 MS + 0.1 mg/L IBA, the rooting rate can reach 100%, and the survival rate of sterile and virus-free seedlings transplanted into a cultivation medium of peat:vermiculite:perlite (1:1:1) is 100%. This experiment aims to establish a fast propagation system for red strawberry tissue culture, in order to provide technical support for the large-scale breeding and industrial production of excellent strawberry varieties.展开更多
研究格尔木河流域水文循环过程并预测未来流域水资源的变化特征,对地区生态环境保护和下游盐湖矿产资源可持续开发利用具有重要意义。选取格尔木水文站以上区域构建SWAT(Soil and Water Assessment Tool)分布式水文模型。采用大气同化...研究格尔木河流域水文循环过程并预测未来流域水资源的变化特征,对地区生态环境保护和下游盐湖矿产资源可持续开发利用具有重要意义。选取格尔木水文站以上区域构建SWAT(Soil and Water Assessment Tool)分布式水文模型。采用大气同化数据集为气象驱动,联合区域内纳赤台和格尔木水文站的实测月尺度径流数据进行参数的率定和验证。在率定期和验证期内,纳什效率系数、确定性系数和相对偏差系数均达到了良好的标准,表明SWAT模型在格尔木河高寒山区流域水文过程模拟中具有较好的适用性。研究表明流域降水量偏少,地表径流量、壤中流量与降水量的变化趋势具有较好的一致性,降水量年际变化中蒸散发量为主要消耗量,占40.26%。根据未来气候预测模型RegCM4.6,预测路径浓度RCP2.6、RCP4.5和RCP8.53种情景下格尔木河未来40年径流量呈增加趋势。3种情景下的年平均径流量较基准期(2006—2018年)分别增加了7.63%、11.01%、15.96%;随着温室气体排放浓度的增加,径流量呈现出增加趋势,特别是夏秋季增幅较大。短时间内径流量增大可能会引发格尔木市洪涝灾害,破坏盐湖企业生产设施;但若将洪水资源进行调控和利用,不仅防范了洪涝灾害,同时也利于解决盐湖企业日渐增大的用水需求难题。展开更多
文摘以红颜草莓匍匐茎为外植体,采取热处理脱毒和微茎尖脱毒相结合、直接微茎尖脱毒两种脱毒方法,以次氯酸钠为消毒剂,探索不同脱毒方法、消毒时间、消毒浓度对无菌脱毒苗获得的影响,得出在5% NaClO,12 min处理后,直接进行微茎尖脱毒,成活率能达到95%以上且长势最优,有明显生长情况。以MS为基本培养基,探索最佳增殖和生根培养基,增殖培养基为MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA时,增值系数为3.4。生根培养基为1/2 MS + 0.1 mg/L IBA时,生根率能达到100%,无菌脱毒苗移栽到草炭:蛭石:珍珠岩(1:1:1)的栽培基质中成活率为100%。本试验建立红颜草莓组培快繁体系,以期为草莓优良品种规模化繁育和工厂化生产提供技术支撑。Using the creeping stems of red strawberry as explants, two detoxification methods were adopted, including heat treatment detoxification and micro stem tip detoxification, and direct micro stem tip detoxification. Sodium hypochlorite was used as the disinfectant to explore the effects of different detoxification methods, disinfection time, and disinfection concentration on obtaining sterile and detoxified seedlings. It was found that after 12 minutes of treatment with 5% NaClO, direct micro stem tip detoxification resulted in a survival rate of over 95% and optimal growth, with significant growth. Using MS as the basic culture medium, explore the optimal proliferation and rooting culture medium. When the proliferation medium is MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA, the value-added coefficient is 3.4. When the rooting medium is 1/2 MS + 0.1 mg/L IBA, the rooting rate can reach 100%, and the survival rate of sterile and virus-free seedlings transplanted into a cultivation medium of peat:vermiculite:perlite (1:1:1) is 100%. This experiment aims to establish a fast propagation system for red strawberry tissue culture, in order to provide technical support for the large-scale breeding and industrial production of excellent strawberry varieties.
文摘研究格尔木河流域水文循环过程并预测未来流域水资源的变化特征,对地区生态环境保护和下游盐湖矿产资源可持续开发利用具有重要意义。选取格尔木水文站以上区域构建SWAT(Soil and Water Assessment Tool)分布式水文模型。采用大气同化数据集为气象驱动,联合区域内纳赤台和格尔木水文站的实测月尺度径流数据进行参数的率定和验证。在率定期和验证期内,纳什效率系数、确定性系数和相对偏差系数均达到了良好的标准,表明SWAT模型在格尔木河高寒山区流域水文过程模拟中具有较好的适用性。研究表明流域降水量偏少,地表径流量、壤中流量与降水量的变化趋势具有较好的一致性,降水量年际变化中蒸散发量为主要消耗量,占40.26%。根据未来气候预测模型RegCM4.6,预测路径浓度RCP2.6、RCP4.5和RCP8.53种情景下格尔木河未来40年径流量呈增加趋势。3种情景下的年平均径流量较基准期(2006—2018年)分别增加了7.63%、11.01%、15.96%;随着温室气体排放浓度的增加,径流量呈现出增加趋势,特别是夏秋季增幅较大。短时间内径流量增大可能会引发格尔木市洪涝灾害,破坏盐湖企业生产设施;但若将洪水资源进行调控和利用,不仅防范了洪涝灾害,同时也利于解决盐湖企业日渐增大的用水需求难题。