[Objective] This study aimed to reveal the therapeutic mechanism of compound sarcandra aerosol, which was exclusively owned by the Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine. [Method...[Objective] This study aimed to reveal the therapeutic mechanism of compound sarcandra aerosol, which was exclusively owned by the Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine. [Method] An inflammation model was established by xylene-induced inflammation test and carrageenan- induced inflammation test to analyze the anti-inflammatory effect of compound sarcandra aerosol. By bacteriostasis test in vitro, the antibacterial effect of compound sarcandra aerosol against five common pathogens of pharyngitis was investigated. Blood samples were collected from Wistar rats in different compound sarcandra aerosol groups and control group to compare blood routine indicators and interleukin-1 （IL-1） levels. [Result] Three different concentrations of compound sarcandra aerosol could reduce degrees of xylene-induced ear edema in mice and carrageenan- induced paw edema in rats, but the anti-inflammatory effect of compound sarcandra aerosol was reduced as the concentration declined. In bacteriostasis test in vitro, the minimum inhibitory concentration of compound sarcandra aerosol against Streptococcus pneumoniae, Streptococcus hemolytis, Corynebacterium diphtheriae, Staphylococcus aureus and Salmonella typhimurium was 76, 105 38, 65 and 30 mg/ml, respectively. Compound sarcandra aerosol reduced white blood cell count, neutrophil count and lymphocyte percentage in pharyngitis model rats. Moreover, interleukin-1 level in watermelon frost lozenge group and different compound sarcandra aerosol groups was lower compared with control group. [Conclusion] Compound sarcandra aerosol can effectively treat pharyngitis by exerting anti-inflammatory and antibacterial effect and reducing interleukin-1 level. This study laid a solid foundation for clinical application of compound sarcandra aerosol.展开更多
[Objective] This study was conducted to establish a near-infrared diffuse reflectance spectroscopy of Guizhou Aspidistra plants. [Method] Twenty three batch- es of Guizhou Aspidistra plants including A. chishuiensis, ...[Objective] This study was conducted to establish a near-infrared diffuse reflectance spectroscopy of Guizhou Aspidistra plants. [Method] Twenty three batch- es of Guizhou Aspidistra plants including A. chishuiensis, A. spinula, A. Caespitosa, A. sichuanensis, A. ebianensis, A. retusa, A. guizhouensis and A. liboensis were subjected to drying, pulverization and sieving and then directly determined for near- infrared reflectance spectrums; and the plants in this genus were classified by clus- ter analysis and principal component analysis （PCA）. [Result] The near-infrared re- flectance spectrums of the 23 batches of Guizhou Aspidistra plants showed very high similarity. The spectrums were processed by first derivative method, and the spectral range of 4 000-7 500 cm-1 was selected as the analytical range. Cluster analysis and PCA were employed to mass spectrum variables of plants in Aspidis- tra, fewer new variables became the linear combination of primary variables, and small differences between different varieties were enlarged, thereby facilitating intu- itive classification of plants in this genus. [Conclusion] Near-infrared diffuse re- flectance spectroscopy is nondestructive and rapid for determination of solid sam- pies, and provides a new method for the classification of Guizhou Aspidistra plants combined by information processing techniques.展开更多
基金Supported by Science and Technology Project of Guizhou Provincial Administration o Traditional Chinese Medicine(QZYY-2014-006)~~
文摘[Objective] This study aimed to reveal the therapeutic mechanism of compound sarcandra aerosol, which was exclusively owned by the Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine. [Method] An inflammation model was established by xylene-induced inflammation test and carrageenan- induced inflammation test to analyze the anti-inflammatory effect of compound sarcandra aerosol. By bacteriostasis test in vitro, the antibacterial effect of compound sarcandra aerosol against five common pathogens of pharyngitis was investigated. Blood samples were collected from Wistar rats in different compound sarcandra aerosol groups and control group to compare blood routine indicators and interleukin-1 （IL-1） levels. [Result] Three different concentrations of compound sarcandra aerosol could reduce degrees of xylene-induced ear edema in mice and carrageenan- induced paw edema in rats, but the anti-inflammatory effect of compound sarcandra aerosol was reduced as the concentration declined. In bacteriostasis test in vitro, the minimum inhibitory concentration of compound sarcandra aerosol against Streptococcus pneumoniae, Streptococcus hemolytis, Corynebacterium diphtheriae, Staphylococcus aureus and Salmonella typhimurium was 76, 105 38, 65 and 30 mg/ml, respectively. Compound sarcandra aerosol reduced white blood cell count, neutrophil count and lymphocyte percentage in pharyngitis model rats. Moreover, interleukin-1 level in watermelon frost lozenge group and different compound sarcandra aerosol groups was lower compared with control group. [Conclusion] Compound sarcandra aerosol can effectively treat pharyngitis by exerting anti-inflammatory and antibacterial effect and reducing interleukin-1 level. This study laid a solid foundation for clinical application of compound sarcandra aerosol.
基金Supported by National Natural Science Foundation of China(81360623)~~
文摘[Objective] This study was conducted to establish a near-infrared diffuse reflectance spectroscopy of Guizhou Aspidistra plants. [Method] Twenty three batch- es of Guizhou Aspidistra plants including A. chishuiensis, A. spinula, A. Caespitosa, A. sichuanensis, A. ebianensis, A. retusa, A. guizhouensis and A. liboensis were subjected to drying, pulverization and sieving and then directly determined for near- infrared reflectance spectrums; and the plants in this genus were classified by clus- ter analysis and principal component analysis （PCA）. [Result] The near-infrared re- flectance spectrums of the 23 batches of Guizhou Aspidistra plants showed very high similarity. The spectrums were processed by first derivative method, and the spectral range of 4 000-7 500 cm-1 was selected as the analytical range. Cluster analysis and PCA were employed to mass spectrum variables of plants in Aspidis- tra, fewer new variables became the linear combination of primary variables, and small differences between different varieties were enlarged, thereby facilitating intu- itive classification of plants in this genus. [Conclusion] Near-infrared diffuse re- flectance spectroscopy is nondestructive and rapid for determination of solid sam- pies, and provides a new method for the classification of Guizhou Aspidistra plants combined by information processing techniques.
