RAPD analysis on Lilium regale and its 13 phenotype male sterility mutants initiated from irradiation bulbs was made by means of 300 random 10mer primers. Of the tested primers, 93 primers produced ideal amplifi...RAPD analysis on Lilium regale and its 13 phenotype male sterility mutants initiated from irradiation bulbs was made by means of 300 random 10mer primers. Of the tested primers, 93 primers produced ideal amplification bands on all materials, 14 primers generated stable different polymorphic bands among mutant ’Wang 2G03’?mutant ’Wang 3G03’ and Lilium regale. 21 different polymorphic bands were found between ’Wang 2G03’ and Lilium regale. 25 different polymorphic bands existed between ’Wang 1G03’ and Lilium regale. It was showed that ’Wang 2G03’ and ’Wang 1G03’were two real different male sterility mutants of Lilium regale. From the 93 primers RAPD bands, the other 11 phenotype male sterility mutants showed no difference in comparison with Lilium regale. It was proposed that more test need to be made to identify whether they were real mutants.展开更多
文摘RAPD analysis on Lilium regale and its 13 phenotype male sterility mutants initiated from irradiation bulbs was made by means of 300 random 10mer primers. Of the tested primers, 93 primers produced ideal amplification bands on all materials, 14 primers generated stable different polymorphic bands among mutant ’Wang 2G03’?mutant ’Wang 3G03’ and Lilium regale. 21 different polymorphic bands were found between ’Wang 2G03’ and Lilium regale. 25 different polymorphic bands existed between ’Wang 1G03’ and Lilium regale. It was showed that ’Wang 2G03’ and ’Wang 1G03’were two real different male sterility mutants of Lilium regale. From the 93 primers RAPD bands, the other 11 phenotype male sterility mutants showed no difference in comparison with Lilium regale. It was proposed that more test need to be made to identify whether they were real mutants.
