异育银鲫因具有食性杂、生长快、肉质好和广适性的优点而发展成为继草鱼、鲤后的又一大吃食性养殖淡水鱼。其中,以盐城为代表的苏北地区是我国异育银鲫养殖密集、产量较高的地区,约占鲫产量的22%(郭闯等,2022;陈静等,2014)。然而,随着...异育银鲫因具有食性杂、生长快、肉质好和广适性的优点而发展成为继草鱼、鲤后的又一大吃食性养殖淡水鱼。其中,以盐城为代表的苏北地区是我国异育银鲫养殖密集、产量较高的地区,约占鲫产量的22%(郭闯等,2022;陈静等,2014)。然而,随着新型病毒Cy HV-2的暴发和蔓延,其与孢子虫一道成了鲫致损最严重的病害(Gao F X等,2017;钱立宇,2021),盐城也是该病害的重灾区之一,使鲫成了一个高发病率、高死亡率和低养殖效益的品种。展开更多
Peptidoglycan recognition proteins（PGRPs） are a family of pattern recognition receptors（PRRs） of the immune system,which bind and hydrolyze bacterial peptidoglycan.Here,a long type PGRP（PGRP-L） was first cloned ...Peptidoglycan recognition proteins（PGRPs） are a family of pattern recognition receptors（PRRs） of the immune system,which bind and hydrolyze bacterial peptidoglycan.Here,a long type PGRP（PGRP-L） was first cloned in the lower vertebrate species Xenopus tropicalis（Xt）.The XtPGRP-L possessed a conserved genomic structure with five exons and four introns.The alignment and phylogenetic analysis indicated that XtPGRP-L might be a type of amidase-like PGRP.The 3-D model showed that XtPGRP-L possessed a conserved structure compared with the Drosophila PGRP-Lb.During embryonic development,XtPGRP-L was not expressed until the 72 h tadpole stage.In adult tissues,it was strongly expressed in the liver,lung,intestine,and stomach.Furthermore,after LPS stimulation,the expression of XtPGRP-L was up-regulated significantly in the liver,intestine and spleen,indicating that XtPGRP-L may play an important role in the innate immunity of Xenopus tropicalis.展开更多
This study cloned the hemoglobin a1 from the marine teleost, the half-smooth tongue sole （Cynoglossus semilaevis）, and then examined its expression under hypoxia exposure. The full-length of CsHb-a1 （594 bp） cDNA ...This study cloned the hemoglobin a1 from the marine teleost, the half-smooth tongue sole （Cynoglossus semilaevis）, and then examined its expression under hypoxia exposure. The full-length of CsHb-a1 （594 bp） cDNA contains an open reading frame encoding 144 amino acids. Sequence analysis shows that the predicted CsHb-a1 amino acids shares high identities with that of other species. Real-time PCR showed that CsHb-a1 was highly expressed in the heart, liver, spleen, kidney and blood. Five to 120 min esposure and long-term （36 h） exposure to hypoxia （1.0 mg/L） significantly increased CsHb-a1 mRNA expression in most tissues compared to those fish held in normoxic conditions （dissolved oxygen （DO）： 6.2 mg/L）. These results suggested that the up-regulation of Hb-a1 is an important component for adaptation of half-smooth tongue sole to short-term hypoxia.展开更多
文摘异育银鲫因具有食性杂、生长快、肉质好和广适性的优点而发展成为继草鱼、鲤后的又一大吃食性养殖淡水鱼。其中,以盐城为代表的苏北地区是我国异育银鲫养殖密集、产量较高的地区,约占鲫产量的22%(郭闯等,2022;陈静等,2014)。然而,随着新型病毒Cy HV-2的暴发和蔓延,其与孢子虫一道成了鲫致损最严重的病害(Gao F X等,2017;钱立宇,2021),盐城也是该病害的重灾区之一,使鲫成了一个高发病率、高死亡率和低养殖效益的品种。
基金supported by the Project from the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB240001)the Foundation for Talent Recruitment of Yancheng Institute of Technology (XKR2011007)the National Natural Science Foundation of China (30830083)
文摘Peptidoglycan recognition proteins（PGRPs） are a family of pattern recognition receptors（PRRs） of the immune system,which bind and hydrolyze bacterial peptidoglycan.Here,a long type PGRP（PGRP-L） was first cloned in the lower vertebrate species Xenopus tropicalis（Xt）.The XtPGRP-L possessed a conserved genomic structure with five exons and four introns.The alignment and phylogenetic analysis indicated that XtPGRP-L might be a type of amidase-like PGRP.The 3-D model showed that XtPGRP-L possessed a conserved structure compared with the Drosophila PGRP-Lb.During embryonic development,XtPGRP-L was not expressed until the 72 h tadpole stage.In adult tissues,it was strongly expressed in the liver,lung,intestine,and stomach.Furthermore,after LPS stimulation,the expression of XtPGRP-L was up-regulated significantly in the liver,intestine and spleen,indicating that XtPGRP-L may play an important role in the innate immunity of Xenopus tropicalis.
基金supported by Nature Science Foundation of Jiangsu Province(BK2011418)the Graduate Research and Innovation of Colleges and Universities in Jiangsu Province(CX09B_106Z)Natural Science Foundation of the Jiangsu Higher Education Institutions of China(09KJD240004)
文摘This study cloned the hemoglobin a1 from the marine teleost, the half-smooth tongue sole （Cynoglossus semilaevis）, and then examined its expression under hypoxia exposure. The full-length of CsHb-a1 （594 bp） cDNA contains an open reading frame encoding 144 amino acids. Sequence analysis shows that the predicted CsHb-a1 amino acids shares high identities with that of other species. Real-time PCR showed that CsHb-a1 was highly expressed in the heart, liver, spleen, kidney and blood. Five to 120 min esposure and long-term （36 h） exposure to hypoxia （1.0 mg/L） significantly increased CsHb-a1 mRNA expression in most tissues compared to those fish held in normoxic conditions （dissolved oxygen （DO）： 6.2 mg/L）. These results suggested that the up-regulation of Hb-a1 is an important component for adaptation of half-smooth tongue sole to short-term hypoxia.
