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Biocontrol of Aspergillus flavus Strains Isolated from Bambara Groundnut (Vigna subterranea (L.) Verdc.) Seeds Using Essential Oils of Lippia multiflora Moldenke, Ocimum americanum L. (Lamiaceae) and Eucalyptus cameldulensis Dehnh
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作者 Mahamadi Nikiema amidou s. ouili +3 位作者 Cheik Omar Tidiane Compaoré Assiètta Ouattara François Palenfo Aboubakar sidiki Ouattara 《Advances in Microbiology》 CAS 2024年第10期555-565,共11页
In nature, plant extracts play a crucial role in defending plants against biotic and abiotic stressors. Moreover, the use of plant-based products, such as plant extracts, represents a promising alternative to syntheti... In nature, plant extracts play a crucial role in defending plants against biotic and abiotic stressors. Moreover, the use of plant-based products, such as plant extracts, represents a promising alternative to synthetic fungicides, which pose potential health risks to consumers. In this study, the antifungal activity of the essential oils (EOs) of Lippia multiflora, Eucalyptus camaldulensis and Ocimum americanum was evaluated against two strains of Aspergillus flavus via the agar dilution method. These two Aspergillus flavus fungi was isolated from Bamabra groundnut seeds. Lippia multiflora essential oil (EO) showed the best results compared with the other oils, with a minimum inhibitory concentration (MIC) of 9000 μg∙mL−1. The MIC for Eucalyptus camaldulensis and Ocimum americanum EOs was 10,800 μg∙mL−1. In view of their antifungal properties, these EOs could be used to develop a new, safe antifungal agent for food preservation. 展开更多
关键词 Aspergillus flavus Essential Oil ANTIFUNGAL Bambara Groundnut
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Purification Potential of Local Media in the Pre-Treatment of Greywater Using Vertical Biofilters under Sahelian Conditions
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作者 Cheik Omar Tidiane Compaoré Ynoussa Maiga +2 位作者 amidou s. ouili Mahamadi Nikiema Aboubakar s. Ouattara 《Journal of Agricultural Chemistry and Environment》 2022年第2期117-131,共15页
Several on-site greywater treatment systems are under development including biofiltration, whose efficiency is influenced by the filter media. Therefore, the main objective of this study was to evaluate the influence ... Several on-site greywater treatment systems are under development including biofiltration, whose efficiency is influenced by the filter media. Therefore, the main objective of this study was to evaluate the influence of the type of filter media and their grain size in the removal of organic and microbial pollutants from greywater. Hence, three types of local filter media of different grain size were used for the pre-treatment of greywater. Their removal potential and clogging time were evaluated and compared. The results indicated that the type of filter media and the grain size have an influence on the elimination of organic and microbial pollution from greywater. Indeed, sand of 1 - 2 mm in size obtained the highest removal efficiencies of organic pollutants (67.35% and 78.04% for COD and BOD5 respectively) and microbial indicators (2.07, 1.77 and 2.27 log. units for E. coli, fecal coliforms and enterococci respectively). Although media of fine texture enhanced the removal efficiencies, they experienced significant clogging problems. To overcome these limitations while enhancing the removal efficiency, 1) pre-treatment stage with coarse materials followed by a treatment with finer materials or 2) the use of a combination of fine and coarse materials should be considered. 展开更多
关键词 BIOFILTRATION Fecal Indicators Granitic Gravel GREYWATER Lateritic Gravel Sand
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Post-Harvest Fungi Associated with Cowpea (Vigna unguiculata L. Walp.) Seeds Produced in Burkina Faso
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作者 amidou s. ouili Ynoussa Maiga +5 位作者 Mahamadi Nikiema souleymane Bissiri Yérobessor Dabiré Illiassou Mogmenga Cheik Omar Tidiane Compaore Aboubakar sidiki Ouattara 《Advances in Microbiology》 2023年第3期148-163,共16页
Cowpea is a very popular foodstuff among people in sub-Saharan Africa. In Burkina Faso, it is the main food legume, especially in rural areas. Its production is facing difficulties including post-harvest losses caused... Cowpea is a very popular foodstuff among people in sub-Saharan Africa. In Burkina Faso, it is the main food legume, especially in rural areas. Its production is facing difficulties including post-harvest losses caused by fungi. Therefore, the objective of this study was to isolate and identify fungal strains associated with cowpea seeds produced in Burkina Faso. Thus, a total of 108 seed samples were collected in the three agro-ecological zones of Burkina Faso. The sanitary analysis of the seeds was carried out using the direct contact method. The isolation and purification of the isolates were performed on Potato Dextrose Agar medium while their identification was done through macroscopic and microscopic phenotypical characterization using different culture media (Potato Dextrose Agar (PDA), Malt Extract Agar (MEA) and Czapeck Dox Agar (CZA)) and different identification keys. A total of 10 fungal species were isolated, with predominance of Aspergillus flavus, Aspegillus niger, Macrophomina phaseolina, Fusarium oxysporium and Rhizopus sp. whose infection rates were 70.8% to 100% of seed samples. In addition to being present in all three zones, the infection rates of Aspergillus flavus (56.55%), Aspergillus niger (20.35%) and Rhizopus (32.80%) were higher in the Sahelian zone. In the Sudano-Sahelian zone, Macrophomina (50.66%) and Fusarium (18.88%) presented the highest infection rates, while Penicillium sp. showed the highest infection rate (2.84%) in the Sudanian zone. This finding demonstrated the necessity to improve post-harvest and conservation techniques of cowpea to limit crop losses and preserve the sanitary quality of this important foodstuff. 展开更多
关键词 COWPEA Seed-Borne Fungi Agro-Ecological Zones Burkina Faso
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