Effect of Arbuscular Mycorrhiza Fungal Inoculation with Compost on Yield and Phosphorous Uptake of Berseem in Alkaline Calcareous Soil

An experiment was conducted in pots under natural conditions in alkaline calcareous soil to determine berseem (Trifolium alexandrium) yield and P uptake as affected by Arbuscular mycorrhizal fungi (AMF) inoculation with compost prepared from fresh animal dung and rock phosphate. Data indicated that berseem shoot and roots yields increased significantly (P ≤ 0.05) by inoculation of indigenous mycorrhiza (AMF-I) and half dose of compost. Shoot yield increased as 98% and 76% roots yield as 60% and 52% over control and N and K fertilizers. Maximum and significantly (P ≤ 0.05) increased plant N and P uptake by berseem was observed in the treatment inoculated by commercial mycorrhiza (AMF-II) with full dose of compost followed by the inoculation of AMF-II with half dose of compost. Plants uptake of Cu, Mn and Fe was improved significantly (P ≤ 0.05) by the inoculation of AMF-II with half dose of compost, while Zn uptake was increased in the treatment of AMF-II inoculation with full dose of compost. Maximum and significantly (P ≤ 0.05) increased soil spores density of AMF as 27 spores per 20 g soil was noted by inoculation of AMF-I with half dose of compost, while maximum roots infection intensity in berseem was observed by the inoculation of AMF-I with full dose of compost. Results suggest that inoculation of AMF with compost has potential to improve berseem yields and plants nutrients uptake under given soil conditions.

Effect of Foliar Application of Micronutrients on Fruit Quality of Peach

This experiment was conducted to study the effect of foliar spray of micronutrients on quality of peach fruits at Horticulture Farm, University of Agriculture Peshawar during 2010. The experiment was conducted in randomized complete design with six treatments and four replications on peach orchards (early grand). Our pre-treatment soil analysis showed silt loam soil class, alkaline and calcareous in nature and adequate in organic matter. The soil was deficient in P, Zn, Fe and B, whereas adequate in Mn and Cu. The fruit quality was evaluated and maximum fruit length, diameter and yield were noted in T6 (Zn + Cu + Fe + Mn + B). The juice pH decreased and the juice acidity increased in a linear fashion after foliar spray of micronutrients. The total soluble solids of fresh fruit juice ranged 7.01%-8.88% and vitamin C ranged from 4.80%-7.90% after foliar spray. So the foliar spray of micronutrients significantly (P ≤ 0.05) affected the quality of peach fruit.

Optimizing Yield and Quality of Canola Cultivars Using Various Potash Levels

The response of several canola cultivars to different potassium (K) levels was investigated in terms of various agronomic parameters including yields, oil and protein contents in a trial conducted at The University of Agriculture, Peshawar-Pakistan in Rabi 2010-11. Three cultivars including Bulbul-98, Abaseen-95 and Dure-NIFA, with five levels of potassium i.e. 0, 30, 60, 90 and 120 kg&middotha-1 were used in Randomized Complete Block (RCB) design with four replications and factorial arrangement. A larger number of days to 50% flowering (116.6 days), plant height (203.8 cm), leaf area index (4.4), 1000 grain weight (3.5 g), biological yield (13189.3 kg&middotha-1), grain yield (1799.2 kg&middotha-1) and harvest index (13.9%) were obtained in K applied plots than the plots where K was not applied. The highest oil (45.1%) and protein (27.7%) was obtained in plots where K was applied at the rate of 120 and 90 kg&middotha-1, respectively. Among cultivars, Bulbul-98 and Abaseen-95 had higher seed yield and oil percentage. Bulbul-98 had more protein content than Abaseen-95 and Durre-NIFA. On average, cultivars gave higher and at par grain yield at 60, 90 and 120 kg&middotha-1. However, they produced higher and at par oil and protein percentage at 90 and 120 kg&middotha-1. Bul-bul-98 and Abaseen-95 are recommended for higher grain yield and oil content for general culti-vation in Peshawar valley. However, for higher protein content, Bulbul-98 is better. Potassium applied at 60 kg&middotha-1 is recommended for higher grain yield, however, for higher oil and protein content, K at 90 kg&middotha-1 is recommended.

Regulatory Genes Through Robust-SNR for Binary Classification Within Functional Genomics Experiments

The current study proposes a novel technique for feature selection by inculcating robustness in the conventional Signal to noise Ratio(SNR).The proposed method utilizes the robust measures of location i.e.,the“Median”as well as the measures of variation i.e.,“Median absolute deviation(MAD)and Interquartile range(IQR)”in the SNR.By this way,two independent robust signal-to-noise ratios have been proposed.The proposed method selects the most informative genes/features by combining the minimum subset of genes or features obtained via the greedy search approach with top-ranked genes selected through the robust signal-to-noise ratio(RSNR).The results obtained via the proposed method are compared with wellknown gene/feature selection methods on the basis of performance metric i.e.,classification error rate.A total of 5 gene expression datasets have been used in this study.Different subsets of informative genes are selected by the proposed and all the other methods included in the study,and their efficacy in terms of classification is investigated by using the classifier models such as support vector machine(SVM),Random forest(RF)and k-nearest neighbors(k-NN).The results of the analysis reveal that the proposed method(RSNR)produces minimum error rates than all the other competing feature selection methods in majority of the cases.For further assessment of the method,a detailed simulation study is also conducted.

Effect of Salts Stress on the Growth and Yield of Wheat (Triticum aestivum L.)

In order to study the effect of salts stress on the growth and yield of wheat (cv. Inqalab), a pot experiment was conducted in the wire-house of the Department of Soil Science, Sindh Agriculture University Tando Jam. The soil was artificially salinized to a range of salinity levels i.e . EC 2.16, 4.0, 6.0, 8.0 and 10.0 dS·m-1 with different salts (MgCl2 + CaCl2 + Na2SO4). The salinized soil used for the experiment was sandy clay in texture, alkaline in reaction (pH > 7.0) and moderate in organic matter (0.95%) content. The results showed that with increasing salinity there was an increase in the ECe, Na+, Ca2+, Mg2+ and Cl- and decrease in the K+, SAR and ESP values of the soil Increasing salinity, progressively decreased plant height, spike length, number of spikelets spike-1 1000 grain weight and yield (straw and grain). Adverse effects of salts on plants were associated with the accumulation of less K+ and more Na+ and Cl- in their flag leaf sap, grains and straw. This resulted in lower K+:5Na+ ratio in flag leaf sap, grains and straw of wheat plants. These results indicated that the effects of salts stress were greater at 10 than at 8, 6 and 4 EC dS·m-1.

Correlation and Regression Analysis for Yield Traits in Wheat (Triticum aestivum L.) Genotypes

The present research work was carried out at Southern Wheat Research Station, Tandojam during the growing season 2013-14 in order to work out correlation and regression analysis for yield traits in wheat genotypes. The experiment was laid out in a Randomized Complete Block Design with three replications. Eight bread wheat genotypes such as Mehran, Sarsabz, TJ-83, SKD-1, Inqlab, A. Sattar, Sehar and Sassui were used to examine correlation and regression. Seven traits, viz.;plant height, tillers plant-1, spike length, spikelet’s spike-1, grains spike-1, seed index and grain yield plant-1 were studied. The analysis of variance revealed significant differences among the genotypes for plant height, tillers plant-1, spike length, spikelet’s spike-1, grains spike-1, seed index and grain yield plant-1. Based on mean performance, variety Sarsabz gave maximum plant height (95.46), produced maximum tillers plant-1 (4.40), gave more grain spike-1 (63.13), TJ-83 gave highest seed index (47.260), and was next ranker in spike length-1 (12.07), spikelet’s spike-1 (19.13), grains spike-1 (59.86), and grain yield plant-1 (9.86) among the genotypes. The phenotypic correlations revealed that tillers plant-1 and grains spike-1 were highly positively associated;hence these yield components can be used as reliable selection criteria to improve grain yield in wheat. Thus estimation of correlation and regression analysis among yield and yield components may provide effective selection criteria to improve wheat grain yield. The results from correlation and regression of plant height indicated significantly positive association with spikelet’s spike-1, tillers plant-1 and grains spike-1 which revealed that increase in plant height will cause corresponding increase in associated traits.

Effect of Different Levels of Phosphorus and Method of Application on the Growth and Yield of Wheat

An experiment was conducted to evaluate the productivity of wheat under different levels of phosphorus and method of application at Department of Agronomy, Sindh Agriculture University, Tandojam. Experiment was laid out in a RCBD (factorial) with three replications. The treatments were four phosphorus levels (P1 = 0 Kg?ha–1 + Recommended Nitrogen, P2 = 30 Kg?ha–1 + Recommended Nitrogen, P3 = 60 Kg?ha–1 + Recommended Nitrogen and P4 = 90 Kg?ha–1 + Recommended Nitrogen) and two placement methods (A1 = Drilling method and A2 = Broadcast method). The statistical analysis of phosphorus levels and placement exhibited significant differences to the maturity days, plant height, tiller production, spike length, grains per spike, seed index, harvest index and grain yield ha–1. However, the interactions of P levels and placement in all crop characters were non-significant except the grain yield/ha–1. The results revealed that application of 90 kg P ha–1 prolonged 143 days for maturity, taller plants (100.16 cm), more tiller plant–1 (13.50), lengthy spikes (12.83 cm), more grains spike–1 (51.33), heavier seed index (48.16 g), better harvest index (50.82%) and satisfactory grain yield (4240.50 Kg?ha–1). The unit increase in grain yield was positively correlated with the increased values of observed crop parameters by extending the values of maturity r = 0.85, plant height r = 0.94, tillers plant r = 0.96, spike length r = 0.95, grains spik–1 r = 0.97, seed index r = 0.95 and harvest index r = 0.97. It was concluded that 90 kg P ha–1 was superior level of phosphorus if, applied with seed drilling for obtaining satisfactory grain yield of wheat crop.

Screening Selected Linseed (Linum usitatissimum L.) Genotypes for Yield Performance in Sindh, Pakistan

The present study was carried out during the year 2013-14 on the screening of the selected linseed genotypes for yield performance in Sindh, Pakistan. The experiment was laid out at the experimental fields of Oilseeds Section, Agriculture Research Institute, Tandojam in a three replicated randomized complete block design. Twelve linseed genotypes (L-53, C-W-2, Shikarpur 2, P-16-4, L-8, 11-106, Hala 3, L-17, Santane, Summit, L-10 and check llsi 90) were screened for their agronomic performance and correlations amongst yield and its contributing traits were calculated. The analysis of variance suggested significant (P < 0.05) variation among linseed genotypes for all the traits studied in this experiment. Most of the genotypes attained flowering and maturity earlier than the check variety;while P-16-4 surpassed Ilsi-90 for primary branches;and most of the remaining genotypes were at par with the commercial check for primary branches. Genotype L-10 surpassed the commercial check for capsules plant-1 and L-8 was at par with the commercial check;while none of the genotypes could surpass commercial check variety for seed index, but 11-106, Hala-3 and Summit also showed promising performance regarding the seed index. All the genotypes tested in this experiment such as L-53, C-W-2, Shikarpur 2, P-16-4, L-8, 11-106, Hala 3, L-17, Santane, Summit, L-10 surpassed the commercial check variety Ilsi-90 for seed yield plot-1. The correlation coefficient for yield and its contributing traits of linseed genotypes showed a positive and significant (P < 0.05) association for primary branches plant-1 vs seed yield plant-1 (r = 0.3334*), days to maturity vs spike length (r = 0.5384**), days to 75% flowering vs days to 90% maturity (r = 0.4073**), days to 75% flowering vs seed index (r = 0.4291**), days to 90% maturity vs seed index (r = 0.3973**) and seed index v/s seed yield plot-1 (r = 0.5160**). Negative and significant (P < 0.05) association was observed for primary branches plant-1 vs days to maturity (r = -0.368*), capsules plant-1 vs days to maturity (r = -0.3567*), days to 90% maturity vs seed yield plant-1 (r = -0.4156**) and days to 90% maturity vs seed yield plot-1 (r = -0.3423*). The new genotypes possess the potential to become future varieties for general cultivation in Sindh province.

Effects of Humic Acid and Crop Residues on Soil and Wheat Nitrogen Contents

This study was conducted with the aim of finding humic acid and crop residues effects on soil and wheat nitrogen at New Development Farm, University of Agriculture, Peshawar, Pakistan during winter 2009-2010. The experiment was laid out in RCB design having four replications. Different levels (1, 1.5, 2 and 2.5 kg ha-1) of Humic acid were applied at sowing time. Mungbean (5 tons ha-1), wheat straw (10 tons ha-1) and mungbean (2.5 tons ha-1) + wheat straw (5 tons ha-1) were incorporated 30 days before sowing. Our results showed higher mineral and total nitrogen (7.32 and 0.43, respectively) mg kg-1 in soil and also higher nitrogen in stem, leaves and grains (2.3, 4.6 and 21.1, respectively) mg kg-1 at maturity in those plots in which 2.5 kg ha-1 of humic acid was applied. Higher mineral and total nitrogen (7.04 and 0.5, respectively) mg kg-1 in soil and also higher nitrogen in leaves (5.5) mg kg-1 at pre-anthesis, while at maturity stage in stem, leaves and grain (5.5, 2.1, 4.2 and 20.8) g kg-1 was recorded in those plots in which 5 tons ha-1 mung bean was incorporated. Our experimental results suggest the use of 5 tons ha-1 of mungbean residues with 2.5 kg ha-1 of humic acid to improve soil fertility availability of more nitrogen in wheat plants to increase the crop yield and grains quality.

Zika: As an emergent epidemic

Zika virus is a new global threat for 2016 that has been swept to almost all Americas and is now posing serious threats to the entire globe.This deadly virus is playing havoc to unborn lives because of its reported association with upsurge of fetal deformation called microcephaly and neuropathic disorders including Guillain-Barrésyndrome.Till today,there is no vaccine prospect,antiviral therapy or licensed medical countermeasures to curb the teratogenic outcomes of this destructive viral infection.Diagnosis,treatment,chronicity and pathogenesis are still vague and unsettled.Therefore,this review article addresses all the aspects related to this disease to mitigate the explosive rise in Zika virus infection.

Mycorrhiza and Phosphate Solubilizing Bacteria:Potential Bioagents for Sustainable Phosphorus Management in Agriculture

Phosphorus(P)is a critical nutrient that plays an essential role in improving soil fertility for optimum plant growth and productivity.It is one of the most deficient macro-nutrients in agricultural soils after nitrogen and is considered inadequate for plant growth and production.To P availability in soils,the farmers are applying huge amounts of synthetic P fertilizers that adversely affect the wider environment,groundwater,soil fertility and microbial population.Many beneficial microbes are known to release and supply soluble P for improving growth and yield of a variety of plants in a sustainable manner in P deficient soils.Thus,inoculation of these microbes,including arbuscular mycorrhizal fungi(AMF)and phosphate solubilizing bacteria(PSB)to soil to enhance crop production without harming the environment,is an alternative approach to chemical fertilizers.The combined role of AMF and PSB in P solubilization is not well understood and the application and mode of action of these microbial groups are often naive due to variation in the environment.Therefore,the current review article would develop a better understanding of the interactive role and mechanisms of AMF and PSB in improving P availability from both organic and inorganic sources in a sustainable crop production system.Finally,the current review would loop out further avenues for researchers interested to commercially produce effective AMF and PSB-based biofertilizers for sustainable management of phosphorus over a wide range of agricultural crops worldwide.

Potential of AM Fungi in Phytoremediation of Heavy Metals and Effect on Yield of Wheat Crop

A pot experiment was conducted to determine the potential of AM fungi in phytoremediation of heavy metals and its effect on yield of wheat crop. The experiment was conducted in CR Design with four replications during rabi 2012-13. Data showed no increase in grain and shoot yields by AMF inoculation with Zn, Cu, Fe, Mn at different levels but increased root yield, plant height, spike length and hundred grains weight of wheat as compared with uninoculated crop. Post-harvest soil Zn, Cu, Fe and Mn contents of 2, 4.4, 2.8 and 2.9 mg·kg-1, respectively were maximum in uninoculated plants treated with Zn, Cu, Fe, Mn at triple of recommended level. No increases in plant P, N, Zn, Cu, Fe and Mn uptakes were observed by the inoculation of AMF when compared with uninoculated crop. Maximum plant Zn, Cu, Fe and Mn uptakes of 160.5, 206, 1914.6 and 2653 g·ha-1, respectively were recorded in uninoculated plants applied with Zn, Cu, Fe, Mn at triple of recommended levels. Wheat roots infection intensity by AMF increased with higher AMF soil spores density. Results suggest the potential of phytoremediation of contaminated soil to be improved by the inoculation of crops with AMF.

Effect of Boron Soil Application on Nutrients Efficiency in Tobacco Leaf

The present study was based on the general hypothesis that boron may affect the accumulation and utilization of other nutrients in plant. For this purpose a field experiment was carried out to find out the influence of boron on the different nutrients content in FCV tobacco (Nicotiana tabacum L.) at TRS Khan Garhi, Mardan, during 2010-2011. Two varieties TM-2008 and Speight G-28 were tested and six levels of boron (0, 0.5, 1, 2, 3 and 5 kg&middotha-1) were applied in the form of boric acid, in randomized complete block design in split plot arrangement and replicated thrice. Results indicated that the yield of tobacco crop increased with 1 kg&middotB&middotha-1 and then decreased sequence wise in both varieties. N and P concentrations were significantly affected by applied boron. Similarly, potassium was increased which is a good indication for a better quality of tobacco crop. Application of boron significantly increased the concentrations of boron nutrients ratios such as K/B;Cl/B and Mn/Fe were decreased while K/Cl and Zn/Cu ratios were increased at lower boron concentrations but decreased at higher concentrations of boron. The fertilizer use efficiency of both the cultivars showed similar trend;however, Speight G-28 was more efficient than TM-2008 in boron accumulation. The overall results revealed that the application of boron should be encouraged for balancing nutrients concentration, thus getting higher yield in the prevailing conditions.

Heterosis and Combing Ability in F1 Population of Hexaploid Wheat (<i>Triticum Aestivum</i>L.)

The heterosis, heterobeltiosis, general combining ability (GCA) and specific combining ability (SCA) were examined during Rabi (crop season) of 2013-2014 in hexaploid wheat genotypes. The experiment for this study was conducted at the Botanical Garden, Department of Plant Breeding & Genetics, Sindh Agriculture University Tandojam, Pakistan. The breeding material was comprised of four parents (Imdad, TD-1, SKD-1, and Moomal). These parents were crossed in half partial mating fashion;thus, six possible cross combinations (F1s) were obtained (Imdad × TD-1, Imdad × SKD-1, Imdad × Moomal, TD-1 × SKD-1, TD-1 × Moomal, and SKD-1 × Moomal). A three-replicated RCB Design was used. The mean squares corresponding to different traits of various hexaploid wheat genotypes indicated significant (P F1 hybrids) effects for the characters (plant height, tillers plant-1, spike length, spikelets spike-1, seeds spike-1, seed index, and grain yield plant-1). The mean performance of F1 hybrids differed significantly (P < 0.01) for all the traits studied. Among the parents, Imdad and TD-1 proved to be better general combiners for almost all the studied traits. In regards to SCA effects, the F1 hybrids Imdad × TD-1 and Imdad × SKD-1 expressed higher SCA and heterotic effects for most of the studied traits.

Study on Growth Effects of Major-Nutrients Composition to Banana Cultivation in Coastal Areas of Sindh, Pakistan

The composition of major nutrients, i.e. nitrogen, phosphorus and potassium (NPK), is a critical factor in banana cultivation and also influences crop yield. This study was aimed to analyze the concentrations of NPK in different banana growing locations of coastal areas of Sindh, Pakistan. A total of 15 composite soil samples at the depth of 0 - 15 and 15 - 30 cm and the same number of banana leaves were collected. The samples were analyzed for the concentration of N, P, K, Ca, Mg, Zn, Mn and Cl concentrations. Results showed that the surface and sub-surface soils of coastal areas were heavy in texture, non-saline, medium alkaline and moderately calcareous in nature. Medium to adequate in Organic Matter (OM), medium in total nitrogen, medium to optimum in available phosphorus, medium to high in exchangeable potassium and deficient in available zinc at both tested depths. The analysis of soluble anions indicated that the carbonates were absent and that chlorides were dominant as compared with bicarbonates. Nitrogen and zinc concentrations of banana leaves were below the critical level, whereas P, K, Mn and chloride ion concentration were above the critical levels. In addition, the relative percentages for K, Ca and Mg were 57.72 to 61.72, 19.05 to 21.00 and 17.70 to 20.00 respectively.

Effect of Water Stress on the Growth and Yield of Cotton Crop (<i>Gossypium hirsutum</i>L.)

Cotton is one of the most important fibre crops playing a key role in economic and social affairs of the world. Water is a critical factor which influences on growth and yield of the cotton crop. Scarcity of water has put tremendous pressure on scientists to introduce drought tolerant cotton varieties. This study was aimed to determine water stress effects on the growth and yield of cotton. The present study was carried out to examine the effect of number of irrigations on the growth and yield of cotton during 2014. The field trial was conducted at the Experimental Fields of Agronomy Section, Agriculture Research Institute, Tandojam, Pakistan. A three replicated Randomized Complete Block Design (RCBD) was employed to layout the experiment having plot size of 2.5 m × 5 m (12.5 m2). Four cotton varieties i.e. NIAB-78, Shahbaz-95, Sindh-1 and TH-224/87 were chosen for this study based on their frequent farmer usage. All the agronomic practices were performed according to standard procedures. Results showed that all the growth and yield components of cotton were significantly (P 0.05) affected by irrigation frequencies. The cotton crop irrigated six times at 21 days interval showed better results with 138.16 cm plant height, 1.45 monopodial branches per plant, 21.83 sympodial branches per plant, 44.58 bolls per plant, 33.86% G.O.T., and 26.97 mm staple length, 149.84 g seed cotton yield per plant and 2271.16 kg·ha-1 seed cotton yield. This study concluded that variety NIAB-78 showed superiority over Shahbaz-95, Sindh-1 and TH-224/87 in almost all the characters of economic importance, and the cotton crop received 6 irrigations at 21 days interval resulted in significantly economical overall performance as compared with 5 irrigations or 4 irrigations with higher seed cotton yield.

An updated systematic review of Zika virus-linked complications

Objective: To determine the breadth of Zika virus(ZIKV)-associated brain anomalies in neonates and adults. Methods: Systematic review was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA) statement using electronic databases ScienceDirect, Pubmed, Medline, Scopus, and Global Health Library.Only those research articles, case studies, case-control studies, case-cohort studies, crosssectional studies, and organizational survey reports were included in the study that reported any fetal outcomes for pregnant women who had infected with ZIKV during the gestational period and ZIKV-related neurological complications in adults as well. Results: Out of total 72 retrieved articles, 50 met the inclusion criteria. We estimated a significant increase in incidence of neural abnormalities such as Guillain-Barre syndrome and microcephaly in the regions that are experiencing ZIKV outbreaks. Other neurological malformations found in ZIKV patients include hydrancephaly/hydrops fetalis, myasthenia gravis,meningoencephalitis and myelitis. Conclusion: Our systematic analysis provides the broad spectrum of neurological malformations in ZIKV infected patients and these data further support the causal link of ZIKV with neurological disorders.

Prediction of promiscuous T-cell epitopes in the Zika virus polyprotein:An in silico approach

Objective:To predict immunogenic promiscuous T-cell epitopes from the polyprotein of the Zika virus using a range of bioinformatics tools.To date,no epitope data are available for the Zika virus in the IEDB database.Methods:We retrieved nearly 54 full length polyprotein sequences of the Zika virus from the NCBI database belonging to different outbreaks.A consensus sequence was then used to predict the promiscuous T cell epitopes that bind MHC 1 and MHC II alleles using Propred1 and Propred immunoinformatic algorithms respectively.The antigencity predicted score was also calculated for each predicted epitope using the Vaxi Jen 2.0 tool.Results:By using Pro Pred1,23 antigenic epitopes for HLA class I and 48 antigenic epitopes for HLA class II were predicted from the consensus polyprotein sequence of Zika virus.The greatest number of MHC class I binding epitopes were projected within the NS5(21%),followed by Envelope(17%).For MHC class II,greatest number of predicted epitopes were in NS5(19%) followed by the Envelope,NS1 and NS2(17% each).A variety of epitopes with good binding affinity,promiscuity and antigenicity were predicted for both the HLA classes.Conclusion:The predicted conserved promiscuous T-cell epitopes examined in this study were reported for the first time and will contribute to the imminent design of Zika virus vaccine candidates,which will be able to induce a broad range of immune responses in a heterogeneous HLA population.However,our results can be verified and employed in future efficacious vaccine formulations only after successful experimental studies.

Advances in research on Zika virus

Zika virus(ZIKV) is rapidly spreading across the America and its devastating outcomes for pregnant women and infants have driven this previously ignored pathogen into the limelight.Clinical manifestations are fever,joint pain or rash and conjunctivitis.Emergence of ZIKV started with a first outbrcak in the Pacific arca in 2007.a second large outbreak occurred in the Pacific in 2013/2014 and subsequently the virus spread in other Pacific islands.Threat of explosive global pandemic and severe clinical complications linked with the more immediate and recurrent epidemics necessitate the development of an effective vaccine.Several vaccine platforms such as DNA vaccine,recombinant subunit vaccine.ZIKV purified inactivated vaccine,and chimeric vaccines have shown potent efficacy in ritro and in rim trials.Moreover,number of drugs such as Sofosbuvir.BCX4450.NITD008 and 7-DMA are ready to enter phase I clinical trial because of proven anti-ZIKV activity.Monoclonal based antibodies offer promise as an intervention effective for use in pregnant women.In this review,we describe the advances in research on ZIKV such as research strategies for the development of antiviral drugs & vaccines,molecular evolution,epidemiology emergence,neurological complications and other teratogenic outcomes as well as pathogenesis.

Radiative properties of matter based on quantum statistical method

We present the preliminary results of our code OPAQS（opacity calculation using quantum statistical model） that is based on the self consistent Hartree-Fock-Slater model for the average atom. The code is capable of performing robust calculations of average charge state, frequency-dependent and mean opacities. The accuracy of the atomic model is verified by comparing the calculations of average charge state with various published results. The monochromatic opacities for iron computed at different sets of temperatures and densities are compared with LEDCOP. The Rosseland and Planck opacities for iron and aluminum are validated with some state-of-the-art codes. The results are in good agreement with the published data.