The journey of multifunctional bone scaffolds fabricated from traditional toward modern techniques

As a bone scaffold,meeting all basic requirements besides dealing with other bone-related issues-bone cancer and accelerated regeneration-is not expected from traditional scaffolds,but a newer class of scaffolds called multifunctional.From a clinical point of view,being a multifunctional scaffold means reducing in healing time,direct costs-medicine,surgery,and hospitalization-and indirect costs-loss of mobility,losing job,and pain.The main aim of the present review is following the multifunctional bone scaffolds trend to deal with both bone regeneration and cancer therapy.Special consideration is given to different fabrication techniques which have been applied to yield these materials spanning from traditional to modern ones.Moreover,the hierarchical structure of bone plus bone cancers and available medicines to them are introduced to familiarize the potential reader of review with the pluri-disciplinary essence of the field.Eventually,a brief discussion relating to the future trend of these materials is provided.

A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration

Recently,the term theragenerative has been proposed for biomaterials capable of inducing therapeutic approaches followed by repairing/regenerating the tissue/organ.This study is focused on the design of a new theragenerative nanocomposite composed of an amphiphilic non-ionic surfactant(Pluronic F127),bioactive glass(BG),and black phosphorus(BP).The nanocomposite was prepared through a two-step synthetic strategy,including a microwave treatment that turned BP nanosheets(BPNS)into quantum dots(BPQDs)with 5±2 nm dimensions in situ.The effects of surfactant and microwave treatment were assessed in vitro:the surfactant distributes the ions homogenously throughout the composite and the microwave treatment chemically stabilizes the composite.The presence of BP enhanced bioactivity and promoted calcium phosphate formation in simulated body fluid.The inherent anticancer activity of BP-containing nanocomposites was tested against osteosarcoma cells in vitro,finding that 150μg mL^(-1)was the lowest concentration which prevented the proliferation of SAOS-2 cells,while the counterpart without BP did not affect the cell growth rate.Moreover,the apoptosis pathways were evaluated and a mechanism of action was proposed.NIR irradiation was applied to induce further proliferation suppression on SAOS-2 cells through hyperthermia.The inhibitory effects of bare BP nanomaterials and nanocomposites on the migration and invasion of bone cancer,breast cancer,and prostate cancer cells were assessed in vitro to determine the anticancer potential of nanomaterials against primary and secondary bone cancers.The regenerative behavior of the nanocomposites was tested with healthy osteoblasts and human mesenchymal stem cells;the BPQDs-incorporated nanocomposite significantly promoted the proliferation of osteoblast cells and induced the osteogenic differentiation of stem cells.This study introduces a new multifunctional theragenerative platform with promising potential for simultaneous bone cancer therapy and regeneration.