Research on the Change of Airfoil Geometric Parameters of Horizontal Axis Wind Turbine Blades Caused by Atmospheric Icing

Icing can significantly change the geometric parameters of wind turbine blades,which in turn,can reduce the aerodynamic characteristics of the airfoil.In-depth research is conducted in this study to identify the reasons for the decline of wind power equipment performance through the icing process.An accurate experimental test method is proposed in a natural environment that examines the growth and distribution of ice formation over the airfoil profile.The mathematical models of the airfoil chord length,camber,and thickness are established in order to investigate the variation of geometric airfoil parameters under different icing states.The results show that ice accumulation varies considerably along the blade span.By environmental temperature drop,the minimum and maximum extents of ice accumulation are observed near the blade root(0.2 R)and the blade tip(0.95 R),respectively(R represents the blade length).The icing process steadily increases the chord length and decreases the airfoil curvature,reaching the largest value at the blade tip region.Furthermore,the maximum curvature is reduced to 41.50%of the original curvature.The maximum camber position of the airfoil moves towards the trailing edge,and the most prominent position occurs at the middle blade region(0.6 R),where it moves back by 19.43%.Ice accumulation steadily increases airfoil thickness.It leads to the maximum thickness growth of 53.40%that occurs at the blade tip region and moves forward to the leading edge by 10%.The research results can provide the required theoretical support for further monitoring the blades operating conditions to ensure reliable wind turbines’operation.

A Hybrid Model Based on Back-Propagation Neural Network and Optimized Support Vector Machine with Particle Swarm Algorithm for Assessing Blade Icing on Wind Turbines

With the continuous increase in the proportional use of wind energy across the globe,the reduction of power generation efficiency and safety hazards caused by the icing on wind turbine blades have attracted more consideration for research.Therefore,it is crucial to accurately analyze the thickness of icing on wind turbine blades,which can serve as a basis for formulating corresponding control measures and ensure a safe and stable operation of wind turbines in winter times and/or in high altitude areas.This paper fully utilized the advantages of the support vector machine(SVM)and back-propagation neural network(BPNN),with the incorporation of particle swarm optimization(PSO)algorithms to optimize the parameters of the SVM.The paper proposes a hybrid assessment model of PSO-SVM and BPNN based on dynamic weighting rules.Three sets of icing data under a rotating working state of the wind turbine were used as examples for model verification.Based on a comparative analysis with other models,the results showed that the proposed model has better accuracy and stability in analyzing the icing on wind turbine blades.

Improving the electrical performances of InSe transistors by interface engineering

InSe has emerged as a promising candidate for next-generation electronics due to its predicted ultrahigh electrical performance.However,the efficacy of the InSe transistor in meeting application requirements is hindered due to its sensitivity to interfaces.In this study,we have achieved notable enhancement in the electrical performance of InSe transistors through interface engineering.We engineered an InSe/h-BN heterostructure,effectively suppressing dielectric layer-induced scattering.Additionally,we successfully established excellent metal-semiconductor contacts using graphene ribbons as a buffer layer.Through a methodical approach to interface engineering,our graphene/InSe/h-BN transistor demonstrates impressive on-state current,field-effect mobility,and on/off ratio at room temperature,reaching values as high as 1.1 mA/μm,904 cm^(2)·V^(-1)·s^(-1),and>10~6,respectively.Theoretical computations corroborate that the graphene/InSe heterostructure shows significant interlayer charge transfer and weak interlayer interaction,contributing to the enhanced performance of InSe transistors.This research offers a comprehensive strategy to elevate the electrical performance of InSe transistors,paving the way for their utilization in future electronic applications.

Anisotropic metal–insulator transition in strained VO_(2)(B) single crystal

Mechanical strain can induce noteworthy structural and electronic changes in vanadium dioxide, imparting substantial scientific importance to both the exploration of phase transitions and the development of potential technological applications. Unlike the traditional rutile(R) phase, bronze-phase vanadium dioxide [VO_(2)(B)] exhibits an in-plane anisotropic structure. When subjected to stretching along distinct crystallographic axes, VO_(2)(B) may further manifest the axial dependence in lattice–electron interactions, which is beneficial for gaining insights into the anisotropy of electronic transport.Here, we report an anisotropic room-temperature metal–insulator transition in single-crystal VO_(2)(B) by applying in-situ uniaxial tensile strain. This material exhibits significantly different electromechanical responses along two anisotropic axes.We reveal that such an anisotropic electromechanical response mainly arises from the preferential arrangement of a straininduced unidirectional stripe state in the conductive channel. This insulating stripe state could be attributed to the in-plane dimerization within the distorted zigzag chains of vanadium atoms, evidenced by strain-modulated Raman spectra. Our work may open up a promising avenue for exploiting the anisotropy of metal–insulator transition in vanadium dioxide for potential technological applications.

A telomere-to-telomere genome assembly of Hongyingzi,a sorghum cultivar used for Chinese Baijiu production

Sorghum(Sorghum bicolor(L.)Moench)is a world cereal crop used in China for producing Baijiu,a distilled spirit.We report a telomere-to-telomere genome assembly of the Baijiu cultivar Hongyingzi,HYZ-T2T,using ultralong reads.The 10 chromosome pairs contained 33,462 genes,of which 93%were functionally annotated.The 20 telomeres and 10 centromeric regions on the HYZ-T2T chromosomes were predicted and two consecutive large inversions on chromosome 2 were characterized.A 65-gene reconstruction of the metabolic pathway of tannins,the flavor substances in Baijiu,was performed and may advance the breeding of sorghum cultivars for Baijiu production.

Ultrasensitive Mechanical Sensor Using Tunable Ordered Array of Metallic and Insulating States in Vanadium Dioxide

Detecting tiny deformations or vibrations, particularly those associated with strains below 1%, is essential in various technological applications. Traditional intrinsic materials, including metals and semiconductors, face challenges in simultaneously achieving initial metallic state and strain-induced insulating state, hindering the development of highly sensitive mechanical sensors. Here we report an ultrasensitive mechanical sensor based on a strain-induced tunable ordered array of metallic and insulating states in the single-crystal bronze-phase vanadium dioxide [VO_(2)(B)] quantum material. It is shown that the initial metallic state in the VO_(2)(B) flake can be tuned to the insulating state by applying a weak uniaxial tensile strain. Such a unique property gives rise to a record-high gauge factor of above 607970, surpassing previous values by an order of magnitude, with excellent linearity and mechanical resilience as well as durability. As a proof-of-concept application, we use our proposed mechanical sensor to demonstrate precise sensing of the micro piece, gentle airflows and water droplets. We attribute the superior performance of the sensor to the strain-induced continuous metal-insulator transition in the single-crystal VO_(2)(B) flake, evidenced by experimental and simulation results. Our findings highlight the potential of exploiting correlated quantum materials for next-generation ultrasensitive flexible mechanical sensors, addressing critical limitations in traditional materials.

Recent advances in studies on changes in Arctic sea ice microstructure and implications to thermodynamic modeling

The study of Arctic sea ice has traditionally been focused on large-scale such as reductions of ice coverage,thickness,volumes and sea ice regime shift.Research has primarily concentrated on the impact of large-scale external factors such as atmospheric and oceanic circulations,and solar radiation.Additionally,Arctic sea ice also undergoes rapid micro-scale evolution such as gas bubbles formation,brine pockets migration and massive formation of surface scattering layer.Field studies like CHINARE(2008-2018)and MOSAiC(2019-2020)have confirmed these observations,yet the full understanding of those changes remain insufficient and superficial.In order to cope better with the rapidly changing Arctic Ocean,this study reviews the recent advances in the microstructure of Arctic sea ice in both field observations and laboratory experiments,and looks forward to the future objectives on the microscale processes of sea ice.The significant porosity and the cyclical annual and seasonal shifts likely modify the ice's thermal,optical,and mechanical characteristics,impacting its energy dynamics and mass balance.Current thermodynamic models,both single-phase and dual-phase,fail to accurately capture these microstructural changes in sea ice,leading to uncertainties in the results.The discrepancy between model predictions and actual observations strongly motivates the parameterization on the evolution in ice microstructure and development of next-generation sea ice models,accounting for changes in ice crystals,brine pockets,and gas bubbles under the background of global warming.It helps to finally achieve a thorough comprehension of Arctic sea ice changes,encompassing both macro and micro perspectives,as well as externaland internal factors.

Brightness temperature simulation of subarctic lake-ice evolution by HIGHTSI and SMRT model

Microwave brightness temperature(TB)can be used to retrieve lake ice thickness in the Arctic and subarctic regions.However,the accuracy of the retrieval is affected by the physical properties of lake ice.To improve the understanding of how lake ice affects TB,numerical modeling was applied.This study combined a physical thermodynamic ice model HIGHTSI with a microwave radiation transfer model SMRT to simulate the TB and lake ice evolution in 2002-2011 in Hulun Lake,China.The reanalyzed meteorological data were used as atmospheric forcing.The ice season was divided into the growth period,the slow growth period,and the ablation period.The simulations revealed that TB was highly sensitive to ice thickness during the ice season,especially vertical polarization measurement at 18.7 GHz.The quadratic polynomial fit for ice thickness to TB outperformed the linear fit,regardless of whether lake ice contained bubbles or not.A comparison of the simulated TB with space-borne TB showed that the simulated TB had the best accuracy during the slow growth period,with a minimum RMSE of 4.6 K.The results were influenced by the bubble radius and salinity.These findings enhance comprehension of the interaction between lake ice properties(including ice thickness,bubbles,and salinity)and TB during ice seasons,offering insights to sea ice in the Arctic and subarctic freshwater observations.

北极夏季海冰表面积雪层内水分含量的时空分布

根据中国第二次北极科学考察实测雪冰温度剖面数据,用一维热传导方程描述雪层温度场,优化辨识了雪层有效热扩散系数和热源。将零热源项作为积雪层中含水和不含水雪层的边界,确定出相应含水和不含水雪层的有效热扩散系数。结合干雪、水物理参数建立雪层内含水量的评估方法并给出北极夏季雪冰温度测试期间雪层内水分含量与温度的关系以及水分变化的时空分布。

Research on Evaluation System of Green Residential Buildings in Cold Areas of Sichuan

With regard to the rapid growth of China’s building area and the increasing energy consumption of buildings, green buildings have become an important issue for balancing economic development and environmental impact. However, the current evaluation systems for various types of green buildings are often unable to achieve a set of standards in practice due to the distinct regional characteristics of each region. Therefore, in view of the regional characteristics of the climate, terrain, ecology, and economic development in the cold regions of Sichuan, it is important to study the evaluation system of green residential buildings suitable for the cold regions of Sichuan. This article focuses on the regional characteristics of climate, topography, ecology, and economic development in the cold regions of Sichuan, and discusses the limitations of the current standards on the practice of green building in cold regions of Sichuan through a sociological questionnaire survey on the comfort of living in the local population. Then from the two dimensions of the advantages of traditional houses and the particularity of national culture, the strategies for the optimization and improvement of the evaluation index system for green residential buildings in the cold regions of Sichuan were proposed. After comprehensively considering the factors that affect the green residential buildings, including the regional characteristics of the cold regions of Sichuan, and the green performance of buildings, local characteristics and ethnic features were included in the evaluation system. The evaluation index system of green residential buildings in the cold regions of Sichuan, consists of 92 three-level indicators. A professional hierarchical analysis software yaahp was used to establish a multi-level hierarchical model between the indicators, and the indicators were compared with each other layer by layer to clarify the importance of the indicators. Based on this, a judgment matrix for each layer was constructed and obtained. The weight of each indicator is accurate, and the scoring mechanism and grading standards are constructed according to it. Through the calculation, the consistency test of the entire model was passed, thereby confirming the scientificity and rationality of the entire evaluation system.

Research on Thermal Comfort of Outdoor Space in Summer Urban Park: Take Mianyang Urban Park as an Example

One of the factors for the evaluation of the space environment is the comfort of outdoor activities spaces in urban parks. The space composed of different landscape elements has different microclimate environment. In this paper, in order to evaluate the role of thermal comfort in influencing resident’s assessment of the outdoor space and activities of the park, a thermal comfort survey was conducted on the outdoor open space of Mianyang Urban Park in summer. In this article, meteorological surveys, questionnaire surveys and observation of park attendance are selected to collect data. The physiological equivalent temperature (PET) assessment was selected as the index to evaluate resident’s thermal comfort level, and the comprehensive evaluation and analysis of the spatial thermal environment of different outdoor landscape elements in the park. The overall comfort of current visitors is mainly influenced by their subjective heat perception voting (TSV). In this article, we focus on providing microclimate adjustment considerations for urban park landscape design, and may help people understand the outdoor thermal comfort of Mianyang in summer, increase the using time of outdoor activities, and promote the use of outdoor space.

积雪密度演变对北极积雪深度模拟的影响

积雪具有复杂的时空分布,在高纬度地区的气-冰-海耦合系统中扮演了重要的角色。准确的积雪质量平衡计算可以帮助我们更好地理解海冰演变过程以及极区冰雪与大气之间的相互作用。雪密度是影响积雪质量平衡众多因素中的重要因子。现有的一维高分辨率冰雪热力学模型(如HIGHTSI)中,使用常数块体雪密度均值将降雪雪水当量转化为积雪深度。本文参考拉格朗日冰上积雪模型(SnowModel-LG)模式对积雪分层压实的处理,简化为新、旧两个雪层,并在质量守恒条件下同时考虑新、旧雪层深度对压实增密的响应,将该物理过程加入HIGHTSI模式中。利用ERA-Interim再分析数据作为大气强迫,针对北极15个冰质量平衡浮标沿其漂移轨迹模拟了降雪积累期海冰表面雪密度变化对积雪深度变化的影响,在原HIGHTSI设置下分别采用定常块体雪密度均值330 kg/m^(3)(T1试验)、接近实际的常数新雪密度200 kg/m^(3)(T2试验)以及改进后框架下新、旧雪层随时间压实增密的雪密度(T3试验)计算积雪深度,并将模拟结果与浮标观测进行对比。结果表明,本文改进的算法对雪密度变化的处理更为合理,且能较好地再现积雪深度的变化;考虑新、旧雪层深度对压实增密的响应能较好地避免以较低的降雪密度持续过度积累,以浮标观测为标准,分层积雪密度压实计算得到的平均绝对误差相对T2减小了5 cm。

MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2

AIM:To investigate the function of microRNA-143(miR-143)in gastric cancer and explore the target genes of miR-143.METHODS:A quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR)analysis was performed to evaluate miR-143 expression in gastric cancer cell lines.After transfecting gastric cancer cells with miR-143-5p and miR-143-3p precursors,Alamar blue and apoptosis assays were used to measure the respective proliferation and apoptosis rates.Cyclooxygenase-2(COX-2)expression was determined by realtime RT-PCR and Western blot assays after miR-143transfection.Reporter plasmids were constructed,and a luciferase reporter assay was used to identify the miR-143 binding site on COX-2.RESULTS:Both miR-143-5p and miR-143-3p were significantly downregulated in multiple gastric cancer cell lines.Forced miR-143-5p and miR-143-3p expression in gastric cancer cells produced a profound cytotoxic effect.MiR-145-5p transfection into gastric cancer cells resulted in a greater growth inhibitory effect(61.23%±3.16%vs 46.58%±4.28%,P<0.05 in the MKN-1cell line)and a higher apoptosis rate(28.74%±1.93%vs 22.13%±3.31%,P<0.05 in the MKN-1 cell line)than miR-143-3p transfection.Further analysis indicated that COX-2 expression was potently suppressed by miR-143-5p but not by miR-143-3p.The activity of a luciferase reporter construct that contained the 3’-untranslated region(UTR)of COX-2 was downregulated by miR-143-5p(43.6%±4.86%,P<0.01)but not by miR-143-3p.A mutation in the miR-145-5p binding site completely ablated the regulatory effect on luciferase activity,which suggests that there is a direct miR-145-5p binding site in the 3’-UTR of COX-2.CONCLUSION:Both miR-143-5p and miR-143-3p function as anti-oncomirs in gastric cancer.However,miR-143-5p alone directly targets COX-2,and it exhibits a stronger tumor suppressive effect than miR-143-3p.

Prognostic value of tertiary lymphoid structure and tumour infiltrating lymphocytes in oral squamous cell carcinoma

Tertiary lymphoid structures(TLS)are ectopic lymphoid structures in cancers that are largely associated with favourable prognosis.However,the prognostic value of TLSs in oral squamous cell carcinoma(OSCC)is largely unknown,and the association between tumour infiltrating lymphocytes(TILs)and TLSs has been rarely explored in OSCC.In this study,associated markers of TLS,including peripheral node address(PNAd)in high endothelial venules,CD20 in B cells and CD3 in T cells,were examined in 168 OSCC patients,and survival analysis was performed between TLS-positive and TLS-negative cohorts.We detected the presence of TILs by staining CD8+cytotoxic T cells and CD57+NK cells as well.TLSs appeared as highly organized structures in 45(26.8%)cases.TLSpositive patients had a better 5-year overall survival(OS)rate(88.9%vs.56.1%,P<0.001)and relapse-free survival(RFS)rate(88.9%vs.63.4%,P=0.002).Moreover,the presence of TLS was an independent prognostic factor for both the 5-year OS rate(hazard ratio[HR]=3.784;95%confidence interval[CI],1.498–9.562)and RFS rate(HR=3.296;95%CI,1.279–8.490)in multivariate analysis.Furthermore,a higher density of CD8+T cells and CD57+NK cells was found in TLS-positive sections than in TLS-negative counterparts(P<0.001),and their combination provided a higher predictive accuracy(AUC=0.730;95%CI,0.654–0.805).In conclusion,our results suggest that TLS is an independent positive prognostic factor for OSCC patients.These findings provide a theoretical basis for the future diagnostic and therapeutic value of TLSs in OSCC treatment.

Autophagy in fate determination of mesenchymal stem cells and bone remodeling

Mesenchymal stem cells(MSCs)have been widely exploited as promising candidates in clinical settings for bone repair and regeneration in view of their self-renewal capacity and multipotentiality.However,little is known about the mechanisms underlying their fate determination,which would illustrate their effectiveness in regenerative medicine.Recent evidence has shed light on a fundamental biological role of autophagy in the maintenance of the regenerative capability of MSCs and bone homeostasis.Autophagy has been implicated in provoking an immediately available cytoprotective mechanism in MSCs against stress,while dysfunction of autophagy impairs the function of MSCs,leading to imbalances of bone remodeling and a wide range of aging and degenerative bone diseases.This review aims to summarize the up-to-date knowledge about the effects of autophagy on MSC fate determination and its role as a stress adaptation response.Meanwhile,we highlight autophagy as a dynamic process and a double-edged sword to account for some discrepancies in the current research.We also discuss the contribution of autophagy to the regulation of bone cells and bone remodeling and emphasize its potential involvement in bone disease.

Orofacial inflammatory pain affects the expression of MT1 and NADPH-d in rat caudal spinal trigeminal nucleus and trigeminal ganglion

Very little is known about the role of melatonin in the trigeminal system, including the function of melatonin receptor 1. In the present study, adult rats were injected with formaldehyde into the right vibrissae pad to establish a model of orofacial inflammatory pain. The distribution of melatonin re- ceptor 1 and nicotinamide adenine dinucleotide phosphate diaphorase in the caudal spinal trigeminal nucleus and trigeminal ganglion was determined with immunohistochemistry and histo- chemistry. The results show that there are significant differences in melatonin receptor 1 expression and nicotinamide adenine dinucleotide phosphate diaphorase expression in the trigeminal ganglia and caudal spinal nucleus during the early stage of orofacial inflammatory pain. Our findings sug- gest that when melatonin receptor 1 expression in the caudal spinal nucleus is significantly reduced, melatonin＇s regulatory effect on pain is attenuated.

Spatial and temporal evolution of landfast ice near Zhongshan Station, East Antarctica, over an annual cycle in 2011/2012

Annual observations of first-year ice(FYI) and second-year ice(SYI) near Zhongshan Station, East Antarctica,were conducted for the first time from December 2011 to December 2012. Melt ponds appeared from early December 2011. Landfast ice partly broke in late January, 2012 after a strong cyclone. Open water was refrozen to form new ice cover in mid-February, and then FYI and SYI co-existed in March with a growth rate of 0.8 cm/d for FYI and a melting rate of 2.7 cm/d for SYI. This difference was due to the oceanic heat flux and the thickness of ice,with weaker heat flux through thicker ice. From May onward, FYI and SYI showed a similar growth by 0.5 cm/d.Their maximum thickness reached 160.5 cm and 167.0 cm, respectively, in late October. Drillings showed variations of FYI thickness to be generally less than 1.0 cm, but variations were up to 33.0 cm for SYI in March,suggesting that the SYI bottom was particularly uneven. Snow distribution was strongly affected by wind and surface roughness, leading to large thickness differences in the different sites. Snow and ice thickness in Nella Fjord had a similar "east thicker, west thinner" spatial distribution. Easterly prevailing wind and local topography led to this snow pattern. Superimposed ice induced by snow cover melting in summer thickened multi-year ice,causing it to be thicker than the snow-free SYI. The estimated monthly oceanic heat flux was ～30.0 W/m2 in March–May, reducing to ～10.0 W/m2 during July–October, and increasing to ～15.0 W/m2 in November. The seasonal change and mean value of 15.6 W/m2 was similar to the findings of previous research. The results can be used to further our understanding of landfast ice for climate change study and Chinese Antarctic Expedition services.

Comprehensive diag”nostic review of the (13)~C-enriched crude oils exemplified by TD2∈ and TZ62S in Tarim Basin, NW China

The crude oils typically from the CambrianLower Ordovician source rocks of Tarim Basin, NW"China, such as TD2 and TZ62S, are13C-enriched with the stable carbon isotopic ratios(VPDB) approaching-28 %.In this paper, the main research viewpoints on this issue are summarized, and combined with results from organic and inorganic carbon isotope stratum curves of the outcrop at the Ya'erdang Mountain in Tarim Basin. In addition, more alternative interpretations are discussed. On one hand, the inverse fractionation features of stable carbon and hydrogen isotopes of these crude oils may imply their protogenous nature. On the other hand, the anisotropy of source rocks and contribution from older stratum source rocks need verifying as well. For the sake of the final resolution of this issue, some further study topics are recommended.

Model simulations of the annual cycle of the landfast ice thickness in the East Siberian Sea

The annual cycle of the thickness and temperature of landfast sea ice in the East Siberian Sea has been examined using a one-dimensional thermodynamic model. The model was calibrated for the year August 2012-July 2013, forced using the data of the Russian weather station Kotel＇ny Island and ECMWF reanalyses. Thermal growth and decay of ice were reproduced well, and the maximum annual ice thickness and breakup day became 1.64 m and the end of July. Oceanic heat flux was 2 W.m^-2 in winter and raised to 25 W.m^-2 in summer, albedo was 0.3-0.8 depending on the surface type （snow/ice and wet/dry）. The model outcome showed sensitivity to the albedo, air temperature and oceanic heat flux. The modelled snow cover was less than 10 cm having a small influence on the ice thickness. In situ sea ice thickness in the East Siberian Sea is rarely available in publications. This study provides a method for quantitative ice thickness estimation by modelling. The result can be used as a proxy to understand the sea ice conditions on the Eurasian Arctic coast, which is important for shipping and high-resolution Arctic climate modelling.

连续膀胱内灌注透明质酸钠联合高压氧仓治疗放射性膀胱炎出血的疗效观察

目的探讨连续膀胱内灌注透明质酸钠联合高压氧仓治疗放射性膀胱炎出血的疗效。方法选取2015年1月—2019年1月在温州市中西医结合医院因盆腔恶性肿瘤接受放射治疗发生放射性膀胱炎出血的患者40例。按照随机分配的原则将患者分为联合治疗组(连续膀胱内灌注透明质酸钠联合高压氧仓)和高压氧组,每组20例。治疗前和治疗后6个月、12个月、18个月评估患者血尿症状、排尿频率及血尿色卡评分(1~10分)。结果两组治疗后6个月、12个月和18个月的完全缓解率和部分缓解率比较,差异无统计学意义(P>0.05)。两组治疗前和治疗后6个月、12个月、18个月的排尿频率和血尿色卡评分在不同时间点、不同组间有差异(P<0.05),联合治疗组的排尿频率和血尿色卡评分较低,相对效果较好。两组排尿频率变化趋势无差异(P>0.05),但血尿色卡评分变化趋势有差异(P<0.05)。治疗6个月时,联合治疗组副作用发生率高于高压氧仓治疗(P<0.05),但治疗12个月和18个月时两组副作用发生率比较,差异无统计学意义(P>0.05)。结论连续膀胱内灌注透明质酸钠联合高压氧仓治疗放射性膀胱炎出血患者具有良好的耐受性,可使膀胱出血、血尿严重程度和排尿频率持续降低至少12个月。