The exocyst is a well-known complex which tethers vesicles at the cell membrane before fusion. Whether an individual subunit can execute a unique function is largely unknown. Using yeast-two-hybrid （Y2H） analysis, w...The exocyst is a well-known complex which tethers vesicles at the cell membrane before fusion. Whether an individual subunit can execute a unique function is largely unknown. Using yeast-two-hybrid （Y2H） analysis, we found that EXO7oA1 interacted with the GOLD domain of Patellin3 （PATL3）. The direct EXO7OA1-PATL3 interaction was supported by in vitro and in vivo experiments. In Arabidopsis, PATL3-GFP colocalized with EXO7oA1 predominantly at the cell membrane, and PATL3 localization was insensitive to BFA and TryA23. Remarkably, in the exo7oa1 mutant, PATL3 proteins accumulated as punctate structures within the cytosol, which did not colocalize with several endomembrane compartment markers, and was insensitive to BFA. Furthermore, PATL3 localization was not changed in the exo7oe2, PRsec6 or exo84b mutants. These data suggested that EXO7oA1, but not other exocyst subunits, was responsible for PATL3 localization, which is independent of its role in secretory/recycling vesicletethering/fusion. Both EXO7oA1 and PATL3 were shown to bind PI4P and PI（4,5）P2 in vitro. Evidence was obtained that the other four members of the PATL family bound to EXO7oA1 as well, and shared a similar localization pattern as PATL3. These findings offered new insights into exocyst subunitspecific function, and provided data and tools for further characterization of PATL family proteins.展开更多
In this paper,the frequency difference of the eigen polarization modes of the Nd:YAG crystal laser at different polarization ratios is experimentally studied,and to the best of our knowledge,the correlation between th...In this paper,the frequency difference of the eigen polarization modes of the Nd:YAG crystal laser at different polarization ratios is experimentally studied,and to the best of our knowledge,the correlation between the frequency difference of the eigenmodes and the output polarization degree is reported for the first time.Combined with the analysis of the polarization beam profile,it is proved that the polarized laser produced by the isotropic crystal is due to the frequency locking of the eigen polarization modes.The weak birefringence in the crystal causes the round-trip phase difference of the orthogonal polarization modes,which leads to the frequency difference between the polarization modes.By the adjustment of the cavity mirror,the anisotropic loss will interact with the round-trip phase difference.The eigen polarization modes can reach frequency degeneration,and then be coherently combined to produce linearly polarized laser output.This work provides a useful reference for understanding the physical mechanism of polarized lasers realized by isotropic crystals.展开更多
End-pumped by a 976 nm diode laser,a high-repetition-rate Er:Yb:YAl3(BO3)4 microchip laser passively Q-switched by a Co2+:MgAl2 O4 crystal is reported.At a quasi-continuous-wave pump power of 20 W,a 1553 nm passively ...End-pumped by a 976 nm diode laser,a high-repetition-rate Er:Yb:YAl3(BO3)4 microchip laser passively Q-switched by a Co2+:MgAl2 O4 crystal is reported.At a quasi-continuous-wave pump power of 20 W,a 1553 nm passively Q-switched laser with the repetition rate of 544 kHz,pulse duration of 8.3 ns,and pulse energy of 3.9 μJ was obtained.To the best of our knowledge,the 544 kHz is the highest reported value for the 1.5 μm passively Q-switched pulse laser.In the continuous-wave pumping experiment,the maximum repetition rate of 144 kHz with the pulse duration of 8.0 ns and pulse energy of 1.7 μJ was obtained at the incident pump power of 6.3 W.展开更多
The behavior of self-polarization emission in Nd∶Y_(3)Al_(5)O_(12)(YAG)=Cr^(4+)∶YAG lasers has been proved in some cases.However,the degree and direction of polarization were often sensitive and unstable.We experime...The behavior of self-polarization emission in Nd∶Y_(3)Al_(5)O_(12)(YAG)=Cr^(4+)∶YAG lasers has been proved in some cases.However,the degree and direction of polarization were often sensitive and unstable.We experimentally observed different beam profiles versus the angle of the polarizer relative to the polarization direction of the laser.In order to explore the polarization mechanism,the dynamics of intracavity polarized eigenmodes was analyzed theoretically.Simulative results were well consistent with our experimental observations.It indicated that the linear self-polarization emission was a composite state rather than an intrinsic state.This study contributed to the improvement of the polarization stability in Nd∶YAG=Cr^(4+)∶YAG passively Q-switched lasers.展开更多
基金supported by grant 31200236 from the National Natural Science Foundation of China(NSFC)Grants KYTZ201402 and KJQN201534 from the Fundamental Research Funds for the Central Universities in China+1 种基金Grant 130809001 from the Jiangsu University Superiority Discipline Construction ProjectThe laboratory of T.M.is cofunded by the Netherlands Organization for Scientific Research(NWO 867.15.020)
文摘The exocyst is a well-known complex which tethers vesicles at the cell membrane before fusion. Whether an individual subunit can execute a unique function is largely unknown. Using yeast-two-hybrid （Y2H） analysis, we found that EXO7oA1 interacted with the GOLD domain of Patellin3 （PATL3）. The direct EXO7OA1-PATL3 interaction was supported by in vitro and in vivo experiments. In Arabidopsis, PATL3-GFP colocalized with EXO7oA1 predominantly at the cell membrane, and PATL3 localization was insensitive to BFA and TryA23. Remarkably, in the exo7oa1 mutant, PATL3 proteins accumulated as punctate structures within the cytosol, which did not colocalize with several endomembrane compartment markers, and was insensitive to BFA. Furthermore, PATL3 localization was not changed in the exo7oe2, PRsec6 or exo84b mutants. These data suggested that EXO7oA1, but not other exocyst subunits, was responsible for PATL3 localization, which is independent of its role in secretory/recycling vesicletethering/fusion. Both EXO7oA1 and PATL3 were shown to bind PI4P and PI（4,5）P2 in vitro. Evidence was obtained that the other four members of the PATL family bound to EXO7oA1 as well, and shared a similar localization pattern as PATL3. These findings offered new insights into exocyst subunitspecific function, and provided data and tools for further characterization of PATL family proteins.
基金supported by the Youth Innovation Promotion Association CAS(No.2022303)the CAS Key Technology Talent Program(No.2022000061)+3 种基金the National Natural Science Foundation of China(Nos.U21A20508,61975208,and 62105334)the Scientific Instrument Developing Project of the Chinese Academy of Sciences(No.YZLY202001)the Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China(Nos.2021ZR203,2020ZZ108,and 2021ZZ118)the Project of Science and Technology of Fujian Province(No.2021H0047)。
文摘In this paper,the frequency difference of the eigen polarization modes of the Nd:YAG crystal laser at different polarization ratios is experimentally studied,and to the best of our knowledge,the correlation between the frequency difference of the eigenmodes and the output polarization degree is reported for the first time.Combined with the analysis of the polarization beam profile,it is proved that the polarized laser produced by the isotropic crystal is due to the frequency locking of the eigen polarization modes.The weak birefringence in the crystal causes the round-trip phase difference of the orthogonal polarization modes,which leads to the frequency difference between the polarization modes.By the adjustment of the cavity mirror,the anisotropic loss will interact with the round-trip phase difference.The eigen polarization modes can reach frequency degeneration,and then be coherently combined to produce linearly polarized laser output.This work provides a useful reference for understanding the physical mechanism of polarized lasers realized by isotropic crystals.
基金supported by the National Natural Science Foundation of China (Nos.61875199 and 61975208)Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDB20000000)Science and Technology Service Network Initiative of the Chinese Academy of Sciences (No.KFJ-STS-QYZX-069)。
文摘End-pumped by a 976 nm diode laser,a high-repetition-rate Er:Yb:YAl3(BO3)4 microchip laser passively Q-switched by a Co2+:MgAl2 O4 crystal is reported.At a quasi-continuous-wave pump power of 20 W,a 1553 nm passively Q-switched laser with the repetition rate of 544 kHz,pulse duration of 8.3 ns,and pulse energy of 3.9 μJ was obtained.To the best of our knowledge,the 544 kHz is the highest reported value for the 1.5 μm passively Q-switched pulse laser.In the continuous-wave pumping experiment,the maximum repetition rate of 144 kHz with the pulse duration of 8.0 ns and pulse energy of 1.7 μJ was obtained at the incident pump power of 6.3 W.
基金supported by the National Natural Science Foundation of China(Nos.61575199,61875199,6197520851761135115)+1 种基金Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB20000000)Science Foundation of Fujian Province(No.2019J02015)。
文摘The behavior of self-polarization emission in Nd∶Y_(3)Al_(5)O_(12)(YAG)=Cr^(4+)∶YAG lasers has been proved in some cases.However,the degree and direction of polarization were often sensitive and unstable.We experimentally observed different beam profiles versus the angle of the polarizer relative to the polarization direction of the laser.In order to explore the polarization mechanism,the dynamics of intracavity polarized eigenmodes was analyzed theoretically.Simulative results were well consistent with our experimental observations.It indicated that the linear self-polarization emission was a composite state rather than an intrinsic state.This study contributed to the improvement of the polarization stability in Nd∶YAG=Cr^(4+)∶YAG passively Q-switched lasers.
