Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(...Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six environments.Seven major QTLs,QGl.cib-2D,QGw.cib-2D,QGw.cib-3B,QGw.cib-4B.1,QGlw.cib-2D.1,QTgw.cib-2D.1 and QTgw.cib-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic variance.Significant interactions were detected between the two major TGW QTLs and three major GW loci.In addition,QTgw.cib-3B.1 and QGw.cib-3B were co-located,and the improved TGW at this locus was contributed by GW.Unlike other loci,QTgw.cib-3B.1/QGw.cib-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that QTgw.cib-3B.1/QGw.cib-3B is likely a novel locus.Six haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and cultivars.According to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of QTgw.cib-3B.1/QGw.cib-3B was predicted.Collectively,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.展开更多
There is an urgent need for novel processes that can integrate different functional nanostructures onto specific substrates,so as to meet the fast-growing need for broad applications in nanoelectronics,nanophotonics,a...There is an urgent need for novel processes that can integrate different functional nanostructures onto specific substrates,so as to meet the fast-growing need for broad applications in nanoelectronics,nanophotonics,and fexible optoelectronics.Existing direct-lithography methods are difficult to use on fexible,nonplanar,and biocompatible surfaces.Therefore,this fabrication is usually accomplished by nanotransfer printing.However,large-scale integration of multiscale nanostructures with unconventional substrates remains challenging because fabrication yields and quality are often limited by the resolution,uniformity,adhesivity,and integrity of the nanostructures formed by direct transfer.Here,we proposed a resist-based transfer strategy enabled by near-zero adhesion,which was achieved by molecular modification to attain a critical surface energy interval.This approach enabled the intact transfer of wafer-scale,ultrathin-resist nanofilms onto arbitrary substrates with mitigated cracking and wrinkling,thereby facilitating the in situ fabrication of nanostructures for functional devices.Applying this approach,fabrication of three-dimensional-stacked multilayer structures with enhanced functionalities,nanoplasmonic structures with~10 nm resolution,and MoS2-based devices with excellent performance was demonstrated on specific substrates.These results collectively demonstrated the high stability,reliability,and throughput of our strategy for optical and electronic device applications.展开更多
NiTiCu-based shape memory alloys have been considered as ideal materials for solid-state refrigeration due to their superb cycling stability for elastocaloric effect.However,the embrittlement and deterioration caused ...NiTiCu-based shape memory alloys have been considered as ideal materials for solid-state refrigeration due to their superb cycling stability for elastocaloric effect.However,the embrittlement and deterioration caused by secondary phase and coarse grains restrict their applications,and it is still challenging since the geometric components are required.Here,bulk NiTiCuCo parts with excellent forming quality were fabricated by laser powder bed fusion(LPBF)technique.The as-fabricated alloy exhibits refined three-phases hierarchical microcomposite formed based on the rapid cooling mode of LPBF,composed of intricate dendritic Ti2Ni–NiTi composite and nano Ti2Cu embedded inside the NiTi-matrix.This configuration endows far superior elastocaloric stability compared to the as-cast counterpart.The low fatigue stems from the strong elastic coupling between the interphases with reversible martensite transformation,revealed by in-situ synchrotron high-energy x-ray diffraction.The fabrication of NiTiCuCo alloy via LPBF fills the bill of complex geometric structures for elastocaloric NiTiCu alloys.The understanding of interphase micro-coupling could provide the guide for designing LPBF fabricated shape memory-based composites,enabling their applications for special demands on other functionalities.展开更多
Diamond has an ultrawide bandgap with excellent physical properties,such as high critical electric field,excellent thermal conductivity,high carrier mobility,etc.Diamond with a hydrogen-terminated(H-terminated)surface...Diamond has an ultrawide bandgap with excellent physical properties,such as high critical electric field,excellent thermal conductivity,high carrier mobility,etc.Diamond with a hydrogen-terminated(H-terminated)surface has a negative electron affinity(NEA)and can easily produce surface electrons from valence or trapped electrons via optical absorption,thermal heating energy or carrier transport in a PN junction.The NEA of the H-terminated surface enables surface electrons to emit with high efficiency into the vacuum without encountering additional barriers and promotes further development and application of diamond-based emitting devices.This article reviews the electron emission properties of H-terminated diamond surfaces exhibiting NEA characteristics.The electron emission is induced by different physical mechanisms.Recent advancements in electron-emitting devices based on diamond are also summarized.Finally,the current challenges and future development opportunities are discussed to further develop the relevant applications of diamond-based electronemitting devices.展开更多
BACKGROUND Cataracts pose a significant clinical burden due to their complex pathogenesis.In recent years,an increase in cataracts coexisting with myopia has heightened the incidence of retinopathy and posterior vitre...BACKGROUND Cataracts pose a significant clinical burden due to their complex pathogenesis.In recent years,an increase in cataracts coexisting with myopia has heightened the incidence of retinopathy and posterior vitreous detachment.Additionally,symptoms of ocular axis elongation,lens nucleus hardening,and vitreous liquefaction have become more prevalent.While conventional extracapsular cataract extraction is commonly employed,it often yields suboptimal visual outcomes.Subsequent advancements in cataract phacoemulsification and lens implantation surgeries have gained widespread acceptance for their ability to improve refraction and significantly improve uncorrected visual acuity.AIM To investigate the effect of capsular treatment after phacoemulsification lens implantation in myopic patients with cataract.METHODS We selected 110 patients(with 134 eyes)with myopia and cataracts treated.These patients were categorized into two groups:an observation group(57 patients with 70 eyes)and a control group(53 patients with 64 eyes).The control group underwent cataract phacoemulsification and lens implantation,while the observation group received a refined capsular treatment based on the control group’s procedure.We assessed the differences in visual acuity and quality between the two groups before and after surgery.RESULTS At six months post-operation,the observation group exhibited significantly improved far vision,intermediate vision,near vision,lower objective scattering index,higher Modulation transfer function cut-off frequency,and overall vision metrics at different contrast levels(100%,20%and 9%)compared to the control group(P<0.05).The total score of the National Eye Institute Visual Function Questionnaire in the observation group at 6 months after operation was significantly higher than that in the control group(P<0.05).No significant difference in the incidence of adverse reactions was observed between the observation group and control group(P>0.05).CONCLUSION Capsular treatment demonstrates efficacy in improving visual acuity and quality after phacoemulsification lens implantation in myopic patients with cataracts,warranting its clinical application.展开更多
BACKGROUND Cognitive dysfunction is the main manifestation of central neuropathy.Although cognitive impairments tend to be overlooked in patients with diabetes mellitus(DM),there is a growing body of evidence linking ...BACKGROUND Cognitive dysfunction is the main manifestation of central neuropathy.Although cognitive impairments tend to be overlooked in patients with diabetes mellitus(DM),there is a growing body of evidence linking DM to cognitive dysfunction.Hyperglycemia is closely related to neurological abnormalities,while often disregarded in clinical practice.Changes in cerebral neurotransmitter levels are associated with a variety of neurological abnormalities and may be closely related to blood glucose control in patients with type 2 DM(T2DM).AIM To evaluate the concentrations of cerebral neurotransmitters in T2DM patients exhibiting different hemoglobin A1c(HbA1c)levels.METHODS A total of 130 T2DM patients were enrolled at the Department of Endocrinology of Shanghai East Hospital.The participants were divided into four groups according to their HbA1c levels using the interquartile method,namely Q1(<7.875%),Q2(7.875%-9.050%),Q3(9.050%-11.200%)and Q4(≥11.200%).Clinical data were collected and measured,including age,height,weight,neck/waist/hip circumferences,blood pressure,comorbidities,duration of DM,and biochemical indicators.Meanwhile,neurotransmitters in the left hippocampus and left brainstem area were detected by proton magnetic resonance spectroscopy.RESULTS The HbA1c level was significantly associated with urinary microalbumin(mALB),triglyceride,low-density lipoprotein cholesterol(LDL-C),homeostasis model assessment of insulin resistance(HOMA-IR),and beta cell function(HOMA-β),N-acetylaspartate/creatine(NAA/Cr),and NAA/choline(NAA/Cho).Spearman correlation analysis showed that mALB,LDL-C,HOMA-IR and NAA/Cr in the left brainstem area were positively correlated with the level of HbA1c(P<0.05),whereas HOMA-βwas negatively correlated with the HbA1c level(P<0.05).Ordered multiple logistic regression analysis showed that NAA/Cho[Odds ratio(OR):1.608,95%confidence interval(95%CI):1.004-2.578,P<0.05],LDL-C(OR:1.627,95%CI:1.119-2.370,P<0.05),and HOMA-IR(OR:1.107,95%CI:1.031-1.188,P<0.01)were independent predictors of poor glycemic control.CONCLUSION The cerebral neurotransmitter concentrations in the left brainstem area in patients with T2DM are closely related to glycemic control,which may be the basis for the changes in cognitive function in diabetic patients.展开更多
In the present study,three types of bimetal composites,Al 6082 sleeve/Al 6082 core,Mg AZ31 sleeve/Mg AZ31 core and Al 6082 sleeve/Mg AZ31 core,were fabricated by drilling and assembling.The rule of mixtures(ROM)for th...In the present study,three types of bimetal composites,Al 6082 sleeve/Al 6082 core,Mg AZ31 sleeve/Mg AZ31 core and Al 6082 sleeve/Mg AZ31 core,were fabricated by drilling and assembling.The rule of mixtures(ROM)for the flow curves and yield strengths during compressive test were addressed.Our results show that the ROM can predict well the experimental flow curves and yield strengths of bimetal composites without bonding,irrespectively of the different strain hardening behavior between the two components.展开更多
Objective:To investigate the effect of BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite on repairing rat critical-sized cranial defects.Methods:The chitosan nanometer hydroxyapatit...Objective:To investigate the effect of BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite on repairing rat critical-sized cranial defects.Methods:The chitosan nanometer hydroxyapatite collagen composite was prepared and the microcosmic appearance of the composite was observed by scanning electron microscope.The BMP-7 derived-peptide was introduced into the composite by vacuum adsorption.The released peptide content from the scaffold was detected using high performance liquid chromatography at different set times.Critical-sized cranial defects were created on both sides of the parietal bone in 24 adult Sprague-Dawley rats.The BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composites were implanted on the right side as experimental group and the left side was implanted with chitosan nanometer hydroxyapatite biomimetic collagen composites alone as control group.The rats of both groups were killed in batch respectively after 6 and 12 weeks.Macroscopic observation,three-dimensional reconstruction of computed tomography(CT)and histological observation were performed on these samples.Results:The results of scanning electron microscope showed that the surface of the scaffold was porous.The releasing character of BMP-7 derived-peptide belonged to slow release.The result of animal experiment showed that the BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite could more effectively promote the repair of cranial bone defects comparing with the chitosan nanometer hydroxyapatite biomimetic collagen composite alone.The difference was statistically significant(p<.05).Conclusions:The BMP-7 derived-peptide chitosan nanometer hydroxyapatite collagen biomimetic composite can effectively promote bone regeneration of bone defects.The composite is a kind of ideal scaffold material for bone tissue engineering.展开更多
Complex-amplitude holographic metasurfaces(CAHMs)with the flexibility in modulating phase and amplitude profiles have been used to manipulate the propagation of wavefront with an unprecedented level,leading to higher ...Complex-amplitude holographic metasurfaces(CAHMs)with the flexibility in modulating phase and amplitude profiles have been used to manipulate the propagation of wavefront with an unprecedented level,leading to higher image-reconstruction quality compared with their natural counterparts.However,prevailing design methods of CAHMs are based on Huygens-Fresnel theory,meta-atom optimization,numerical simulation and experimental verification,which results in a consumption of computing resources.Here,we applied residual encoder-decoder convolutional neural network to directly map the electric field distributions and input images for monolithic metasurface design.A pretrained network is firstly trained by the electric field distributions calculated by diffraction theory,which is subsequently migrated as transfer learning framework to map the simulated electric field distributions and input images.The training results show that the normalized mean pixel error is about 3%on dataset.As verification,the metasurface prototypes are fabricated,simulated and measured.The reconstructed electric field of reverse-engineered metasurface exhibits high similarity to the target electric field,which demonstrates the effectiveness of our design.Encouragingly,this work provides a monolithic field-to-pattern design method for CAHMs,which paves a new route for the direct reconstruction of metasurfaces.展开更多
Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was construct...Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was constructed to detect QTL for thousand-grain weight(TGW),grain length(GL)and grain width(GW)across eight environments.Genomic regions associated with grain size and grain weight were identified on chromosomes 4A and 6A using bulked segregant exome sequencing(BSE-Seq)analysis.After constructing genetic maps,six major QTL detected in at least four individual environments and in best linear unbiased estimator(BLUE)datasets,explained 7.50%-23.45%of the phenotypic variation.Except for QGl.cib-4A,the other five QTL were co-located in two regions,namely QTgw/Gw.cib-4A and QTgw/Gw/Gl.cib-6A.Interactions of these QTL were analyzed.Unlike QTgw/Gw/Gl.cib-6A,QTgw/Gw.cib-4A and QGl.cib-4A had no effect on grain number per spike(GNS).The QTL were validated in a second cross using Kompetitive Allele Specific PCR(KASP)markers.Since QTgw/Gw.cib-4A was probably a novel locus,it and the KASP markers reported here can be used in wheat breeding.TraesCS4A03G0191200 was predicted to be potential candidate gene for QTgw/Gw.cib-4A based on the sequence differences,spatiotemporal expression patterns,gene annotation and haplotype analysis.Our findings will be useful for fine mapping and for marker-assisted selection in wheat grain yield improvement.展开更多
Owing to the depletion of wolframite, the focus of tungsten extraction has gradually shifted to scheelite. However, separating the associated minerals(e.g., apatite, fluorite, and calcite) and scheelite is challenging...Owing to the depletion of wolframite, the focus of tungsten extraction has gradually shifted to scheelite. However, separating the associated minerals(e.g., apatite, fluorite, and calcite) and scheelite is challenging because their surface physicochemical properties are similar to those of scheelite. Fortunately, researchers have made substantial progress in separating the minerals of scheelite by using depressants. This study reviews the application and inhibition mechanism of inorganic depressants in obtaining tungsten from its calcium-bearing minerals. The application of new organic depressants in obtaining tungsten from its calcium-bearing minerals and the associated mechanisms are also summarized. After an objective assessment of inorganic and organic depressants’ advantages and disadvantages, possible future research directions for inorganic and organic depressants are proposed. Herein, we provide a theoretical basis for developing scheelite flotation depressants.展开更多
Hot dry rock(HDR)geothermal energy is a kind of widely distributed clean energy with huge reserves.However,its commercial development has been constrained by reservoir stimulation.In the early stage of HDR geothermal ...Hot dry rock(HDR)geothermal energy is a kind of widely distributed clean energy with huge reserves.However,its commercial development has been constrained by reservoir stimulation.In the early stage of HDR geothermal energy development,properly determining spatial distribution patterns of natural fractures in HDR reservoirs can effectively guide reservoir stimulation.This study analyzes the spatial distribution of natural fractures by using FracMan software based on the actual geological data and log data of well M-2 in the Matouying Uplift area,Hebei Province.The fracture parameters are counted and Monte Carlo simulation technique is introduced to optimize the parameters,which makes the natural fracture model more accurate and reliable.Furthermore,this study simulates hydraulic fracturing using the model combined with the actual in-situ stress parameters and the construction scheme.As verified by fitting the changes in simulated wellhead pressure during hydraulic fracturing with the actual wellhead pressure data detected during construction,the methods for natural fracture modeling used in this study are scientific and reasonable.The preliminary prediction results show that the displacement design scheme with a pump displacement of 2.0-3.0 m^(3)/min,4.0-5.5 m^(3)/min and 6-7 m^(3)/min in the early,middle and late stages,respectively,has good fracturing effect.The results of this study can be utilized as a reference for preparing development schemes for HDR reservoirs.展开更多
Ultrasonic peen forming(UPF)is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming metho...Ultrasonic peen forming(UPF)is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming methods.Although ultrasonic transducers with longitudinal vibration have been widely studied,they have seldom been incorporated into UPF devices for machining in confined spaces.To meet the requirements of this type of machining,a sandwich-type piezoelectric transducer with coupled longitudinal-flexural vibrational modes is proposed.The basic structure of the transducer is designed to obtain large vibrational amplitudes in both modes.Experimental results obtained with a prototype device demonstrate the feasibility of the proposed transducer.The measured vibrational amplitude for the working face in the longitudinal vibrational mode is 1.0μm,and electrical matching increases this amplitude by 40%.The flexural vibration characteristics of the same prototype transducer are also tested and are found to be slightly smaller than those of longitudinal mode.The resultant working strokes of the UPF impact pins reach 1.7 mm and 1.2 mm in the longitudinal and flexural modes,respectively.The forming capability of the prototype has been evaluated via 15-min machining on standard 2024-T351 aluminum plates.After UPF,an improved surface morphology with lower surface roughness is obtained.The aluminum plate test piece has an apparent upper deformation with an arc height of 0.64 mm.The measured peak value of the compressive residual stress is around 250 MPa,appearing at a depth of 100μm.The proposed longitudinal-flexural hybrid transducer thus provides a high-performance tool for plate peen forming in confined spaces.展开更多
BACKGROUND Serum alanine aminotransferase(ALT) levels are often considered a marker to evaluate liver disease and its severity.AIM To investigate the association between ALT levels and all-cause and cause-specific mor...BACKGROUND Serum alanine aminotransferase(ALT) levels are often considered a marker to evaluate liver disease and its severity.AIM To investigate the association between ALT levels and all-cause and cause-specific mortality in patients with nonalcoholic fatty liver disease(NAFLD).METHODS The Third National Health and Nutrition Examination Survey(NHANES-Ⅲ) from 1988 to 1994 and NHANES-Ⅲ-related mortality data from 2019 onward were used to obtain the necessary data for the study. NAFLD was defined as hepatic steatosis, as diagnosed by ultrasound, with no other liver diseases. ALT levels were categorized into four groups according to the different recommended upper limits of normal(ULN) in men and women: < 0.5 ULN, 0.5-1 ULN, 1-2 ULN, and ≥ 2 ULN. The hazard ratios for all-cause mortality and cause-specific mortality were analyzed using the Cox proportional hazard model.RESULTS Multivariate logistic regression analysis demonstrated that the odds ratio of NAFLD correlated positively with increased serum ALT levels. In patients with NAFLD, all-cause mortality and cardiovascular mortality were the highest when ALT was < 0.5 ULN, yet cancer-related mortality was the highest when ALT was ≥ 2 ULN. The same results could be found in both men and women. Univariate analysis showed that severe NAFLD with normal ALT levels had the highest allcause and cause-specific mortality, but the difference was not statistically significant after adjustment for age and multivariate factors.CONCLUSION The risk of NAFLD was positively correlated with ALT level, but all-cause and cardiovascular mortality were the highest when ALT was < 0.5 ULN. Regardless of the severity of NAFLD, normal or lower ALT levels were associated with higher mortality than elevated ALT levels. Clinicians should be aware that high ALT levels indicate liver injury, but low ALT levels are associated with a higher risk of death.展开更多
Local red culture is an important educational resource.Integrating it into the course of agronomy majors is an important part of cultivating applied talents who grasp and love agriculture in the new era.This paper ana...Local red culture is an important educational resource.Integrating it into the course of agronomy majors is an important part of cultivating applied talents who grasp and love agriculture in the new era.This paper analyzes the necessity of integrating local red culture into courses of agronomy majors,discusses the current challenges,and puts forward specific recommendations.展开更多
In the sewage treatment process,facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic circulation.Cyanoph...In the sewage treatment process,facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic circulation.Cyanophycin granule polypeptide(CGP),a recently discovered high value-added biopolymer present in activated sludge,has provided new avenues for the recovery of resources.However,the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus far.This study investigated the synthesis of CGP,polyhydroxyalkanoates(PHA),and alginate-like exopolysaccharides(ALE)in various microbial aggregates under different carbon sources feeding conditions.Our results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding conditions.Furthermore,biofilm was more conducive to CGP accumulation than floc sludge.Compared with glucose and methanol,sodium acetate significantly enriched the CGP synthetase-encoding gene(cphAabundance=~17419),resulting in the highest CGP yield(average 107.1 mg/g MLSS)in both biofilm and floc sludge.This study is the first to reported the characteristic fluorescence of CGP(Ex/Em=~360/450 nm)caused by the aggregated luminescence of arginine on the side chains.Overall,this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.展开更多
基金the National Key Research and Development Program of China(No.2018YFC1903400)the Key Program for International S&T Cooperation Projects of China(No.2021YFE0106800)+1 种基金the National Natural Science Foundation of China(Nos.52164020,52174248)the Science and Technology Research Project of Education Department of Jiangxi Province,China(No.GJJ190442).
基金supported by the Major Program of National Agricultural Science and Technology of China(NK20220607)the West Light Foundation of the Chinese Academy of Sciences(2022XBZG_XBQNXZ_A_001)the Sichuan Science and Technology Program,China(2022ZDZX0014)。
文摘Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six environments.Seven major QTLs,QGl.cib-2D,QGw.cib-2D,QGw.cib-3B,QGw.cib-4B.1,QGlw.cib-2D.1,QTgw.cib-2D.1 and QTgw.cib-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic variance.Significant interactions were detected between the two major TGW QTLs and three major GW loci.In addition,QTgw.cib-3B.1 and QGw.cib-3B were co-located,and the improved TGW at this locus was contributed by GW.Unlike other loci,QTgw.cib-3B.1/QGw.cib-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that QTgw.cib-3B.1/QGw.cib-3B is likely a novel locus.Six haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and cultivars.According to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of QTgw.cib-3B.1/QGw.cib-3B was predicted.Collectively,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.
基金supported by the National Key Research and Development Program of China(No.2022YFB4602600)the National Natural Science Foundation of China(No.52221001)Hunan Provincial Innovation Foundation for Postgraduate(No.CX20220406)。
文摘There is an urgent need for novel processes that can integrate different functional nanostructures onto specific substrates,so as to meet the fast-growing need for broad applications in nanoelectronics,nanophotonics,and fexible optoelectronics.Existing direct-lithography methods are difficult to use on fexible,nonplanar,and biocompatible surfaces.Therefore,this fabrication is usually accomplished by nanotransfer printing.However,large-scale integration of multiscale nanostructures with unconventional substrates remains challenging because fabrication yields and quality are often limited by the resolution,uniformity,adhesivity,and integrity of the nanostructures formed by direct transfer.Here,we proposed a resist-based transfer strategy enabled by near-zero adhesion,which was achieved by molecular modification to attain a critical surface energy interval.This approach enabled the intact transfer of wafer-scale,ultrathin-resist nanofilms onto arbitrary substrates with mitigated cracking and wrinkling,thereby facilitating the in situ fabrication of nanostructures for functional devices.Applying this approach,fabrication of three-dimensional-stacked multilayer structures with enhanced functionalities,nanoplasmonic structures with~10 nm resolution,and MoS2-based devices with excellent performance was demonstrated on specific substrates.These results collectively demonstrated the high stability,reliability,and throughput of our strategy for optical and electronic device applications.
基金supported by National Key R&D Program of China (2022YFB4600500)the National Safety Academic Fund (U2130201 and U2330105)the NSFC (52201214and 51971244)
文摘NiTiCu-based shape memory alloys have been considered as ideal materials for solid-state refrigeration due to their superb cycling stability for elastocaloric effect.However,the embrittlement and deterioration caused by secondary phase and coarse grains restrict their applications,and it is still challenging since the geometric components are required.Here,bulk NiTiCuCo parts with excellent forming quality were fabricated by laser powder bed fusion(LPBF)technique.The as-fabricated alloy exhibits refined three-phases hierarchical microcomposite formed based on the rapid cooling mode of LPBF,composed of intricate dendritic Ti2Ni–NiTi composite and nano Ti2Cu embedded inside the NiTi-matrix.This configuration endows far superior elastocaloric stability compared to the as-cast counterpart.The low fatigue stems from the strong elastic coupling between the interphases with reversible martensite transformation,revealed by in-situ synchrotron high-energy x-ray diffraction.The fabrication of NiTiCuCo alloy via LPBF fills the bill of complex geometric structures for elastocaloric NiTiCu alloys.The understanding of interphase micro-coupling could provide the guide for designing LPBF fabricated shape memory-based composites,enabling their applications for special demands on other functionalities.
基金the National Natural Sci-ence Foundation of China(Grant No.62274084)the Fun-damental Research Funds for the Central Universities(Grant No.0210-14380193).
文摘Diamond has an ultrawide bandgap with excellent physical properties,such as high critical electric field,excellent thermal conductivity,high carrier mobility,etc.Diamond with a hydrogen-terminated(H-terminated)surface has a negative electron affinity(NEA)and can easily produce surface electrons from valence or trapped electrons via optical absorption,thermal heating energy or carrier transport in a PN junction.The NEA of the H-terminated surface enables surface electrons to emit with high efficiency into the vacuum without encountering additional barriers and promotes further development and application of diamond-based emitting devices.This article reviews the electron emission properties of H-terminated diamond surfaces exhibiting NEA characteristics.The electron emission is induced by different physical mechanisms.Recent advancements in electron-emitting devices based on diamond are also summarized.Finally,the current challenges and future development opportunities are discussed to further develop the relevant applications of diamond-based electronemitting devices.
文摘BACKGROUND Cataracts pose a significant clinical burden due to their complex pathogenesis.In recent years,an increase in cataracts coexisting with myopia has heightened the incidence of retinopathy and posterior vitreous detachment.Additionally,symptoms of ocular axis elongation,lens nucleus hardening,and vitreous liquefaction have become more prevalent.While conventional extracapsular cataract extraction is commonly employed,it often yields suboptimal visual outcomes.Subsequent advancements in cataract phacoemulsification and lens implantation surgeries have gained widespread acceptance for their ability to improve refraction and significantly improve uncorrected visual acuity.AIM To investigate the effect of capsular treatment after phacoemulsification lens implantation in myopic patients with cataract.METHODS We selected 110 patients(with 134 eyes)with myopia and cataracts treated.These patients were categorized into two groups:an observation group(57 patients with 70 eyes)and a control group(53 patients with 64 eyes).The control group underwent cataract phacoemulsification and lens implantation,while the observation group received a refined capsular treatment based on the control group’s procedure.We assessed the differences in visual acuity and quality between the two groups before and after surgery.RESULTS At six months post-operation,the observation group exhibited significantly improved far vision,intermediate vision,near vision,lower objective scattering index,higher Modulation transfer function cut-off frequency,and overall vision metrics at different contrast levels(100%,20%and 9%)compared to the control group(P<0.05).The total score of the National Eye Institute Visual Function Questionnaire in the observation group at 6 months after operation was significantly higher than that in the control group(P<0.05).No significant difference in the incidence of adverse reactions was observed between the observation group and control group(P>0.05).CONCLUSION Capsular treatment demonstrates efficacy in improving visual acuity and quality after phacoemulsification lens implantation in myopic patients with cataracts,warranting its clinical application.
基金Supported by the Academic Leaders Training Program of Pudong Health Bureau of Shanghai,No.PWRd2023-03Clinical Research Fund of Shanghai Municipal Commission of Health,No.202040136+1 种基金National Natural Science Foundation of China,No.82070842Jiangxi Health Commission Science and Technology Plan Project,No.202212838 and No.202212852.
文摘BACKGROUND Cognitive dysfunction is the main manifestation of central neuropathy.Although cognitive impairments tend to be overlooked in patients with diabetes mellitus(DM),there is a growing body of evidence linking DM to cognitive dysfunction.Hyperglycemia is closely related to neurological abnormalities,while often disregarded in clinical practice.Changes in cerebral neurotransmitter levels are associated with a variety of neurological abnormalities and may be closely related to blood glucose control in patients with type 2 DM(T2DM).AIM To evaluate the concentrations of cerebral neurotransmitters in T2DM patients exhibiting different hemoglobin A1c(HbA1c)levels.METHODS A total of 130 T2DM patients were enrolled at the Department of Endocrinology of Shanghai East Hospital.The participants were divided into four groups according to their HbA1c levels using the interquartile method,namely Q1(<7.875%),Q2(7.875%-9.050%),Q3(9.050%-11.200%)and Q4(≥11.200%).Clinical data were collected and measured,including age,height,weight,neck/waist/hip circumferences,blood pressure,comorbidities,duration of DM,and biochemical indicators.Meanwhile,neurotransmitters in the left hippocampus and left brainstem area were detected by proton magnetic resonance spectroscopy.RESULTS The HbA1c level was significantly associated with urinary microalbumin(mALB),triglyceride,low-density lipoprotein cholesterol(LDL-C),homeostasis model assessment of insulin resistance(HOMA-IR),and beta cell function(HOMA-β),N-acetylaspartate/creatine(NAA/Cr),and NAA/choline(NAA/Cho).Spearman correlation analysis showed that mALB,LDL-C,HOMA-IR and NAA/Cr in the left brainstem area were positively correlated with the level of HbA1c(P<0.05),whereas HOMA-βwas negatively correlated with the HbA1c level(P<0.05).Ordered multiple logistic regression analysis showed that NAA/Cho[Odds ratio(OR):1.608,95%confidence interval(95%CI):1.004-2.578,P<0.05],LDL-C(OR:1.627,95%CI:1.119-2.370,P<0.05),and HOMA-IR(OR:1.107,95%CI:1.031-1.188,P<0.01)were independent predictors of poor glycemic control.CONCLUSION The cerebral neurotransmitter concentrations in the left brainstem area in patients with T2DM are closely related to glycemic control,which may be the basis for the changes in cognitive function in diabetic patients.
基金Guangdong Academy of Science Fund(2020GDASYL-20200101001,2018GDASCX-0967,2019GDASYL-0302017 and 2019GDASYL-0502009)National Natural Science Foundation of China(51905111)+1 种基金Guangdong Science and Technology Project(2018dr005)Guangzhou Science and Technology Project(201704030094).
文摘In the present study,three types of bimetal composites,Al 6082 sleeve/Al 6082 core,Mg AZ31 sleeve/Mg AZ31 core and Al 6082 sleeve/Mg AZ31 core,were fabricated by drilling and assembling.The rule of mixtures(ROM)for the flow curves and yield strengths during compressive test were addressed.Our results show that the ROM can predict well the experimental flow curves and yield strengths of bimetal composites without bonding,irrespectively of the different strain hardening behavior between the two components.
文摘Objective:To investigate the effect of BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite on repairing rat critical-sized cranial defects.Methods:The chitosan nanometer hydroxyapatite collagen composite was prepared and the microcosmic appearance of the composite was observed by scanning electron microscope.The BMP-7 derived-peptide was introduced into the composite by vacuum adsorption.The released peptide content from the scaffold was detected using high performance liquid chromatography at different set times.Critical-sized cranial defects were created on both sides of the parietal bone in 24 adult Sprague-Dawley rats.The BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composites were implanted on the right side as experimental group and the left side was implanted with chitosan nanometer hydroxyapatite biomimetic collagen composites alone as control group.The rats of both groups were killed in batch respectively after 6 and 12 weeks.Macroscopic observation,three-dimensional reconstruction of computed tomography(CT)and histological observation were performed on these samples.Results:The results of scanning electron microscope showed that the surface of the scaffold was porous.The releasing character of BMP-7 derived-peptide belonged to slow release.The result of animal experiment showed that the BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite could more effectively promote the repair of cranial bone defects comparing with the chitosan nanometer hydroxyapatite biomimetic collagen composite alone.The difference was statistically significant(p<.05).Conclusions:The BMP-7 derived-peptide chitosan nanometer hydroxyapatite collagen biomimetic composite can effectively promote bone regeneration of bone defects.The composite is a kind of ideal scaffold material for bone tissue engineering.
基金supports from the National Natural Science Foundation of China under Grant Nos.61971435,62101588,62101589Natural Science Basic Research Program of Shaanxi Province(Grant No:2022JM-352,2022JQ-335,2023-JC-YB-069)the National Key Research and Development Program of China(Grant No.:SQ2017YFA0700201).
文摘Complex-amplitude holographic metasurfaces(CAHMs)with the flexibility in modulating phase and amplitude profiles have been used to manipulate the propagation of wavefront with an unprecedented level,leading to higher image-reconstruction quality compared with their natural counterparts.However,prevailing design methods of CAHMs are based on Huygens-Fresnel theory,meta-atom optimization,numerical simulation and experimental verification,which results in a consumption of computing resources.Here,we applied residual encoder-decoder convolutional neural network to directly map the electric field distributions and input images for monolithic metasurface design.A pretrained network is firstly trained by the electric field distributions calculated by diffraction theory,which is subsequently migrated as transfer learning framework to map the simulated electric field distributions and input images.The training results show that the normalized mean pixel error is about 3%on dataset.As verification,the metasurface prototypes are fabricated,simulated and measured.The reconstructed electric field of reverse-engineered metasurface exhibits high similarity to the target electric field,which demonstrates the effectiveness of our design.Encouragingly,this work provides a monolithic field-to-pattern design method for CAHMs,which paves a new route for the direct reconstruction of metasurfaces.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030402)Sichuan Science and Technology Program.
文摘Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was constructed to detect QTL for thousand-grain weight(TGW),grain length(GL)and grain width(GW)across eight environments.Genomic regions associated with grain size and grain weight were identified on chromosomes 4A and 6A using bulked segregant exome sequencing(BSE-Seq)analysis.After constructing genetic maps,six major QTL detected in at least four individual environments and in best linear unbiased estimator(BLUE)datasets,explained 7.50%-23.45%of the phenotypic variation.Except for QGl.cib-4A,the other five QTL were co-located in two regions,namely QTgw/Gw.cib-4A and QTgw/Gw/Gl.cib-6A.Interactions of these QTL were analyzed.Unlike QTgw/Gw/Gl.cib-6A,QTgw/Gw.cib-4A and QGl.cib-4A had no effect on grain number per spike(GNS).The QTL were validated in a second cross using Kompetitive Allele Specific PCR(KASP)markers.Since QTgw/Gw.cib-4A was probably a novel locus,it and the KASP markers reported here can be used in wheat breeding.TraesCS4A03G0191200 was predicted to be potential candidate gene for QTgw/Gw.cib-4A based on the sequence differences,spatiotemporal expression patterns,gene annotation and haplotype analysis.Our findings will be useful for fine mapping and for marker-assisted selection in wheat grain yield improvement.
基金financially supported by the National Natural Science Foundation of China (Nos.52174248 and 52264022)the Natural Science Foundation of Jiangxi Province,China (No.20202ACBL214010)+1 种基金the “Thousand Talents Plan” of Jiangxi Province,China (No.jxsq2019201115)the Youth Jinggang Scholars program in Jiangxi Province,China。
文摘Owing to the depletion of wolframite, the focus of tungsten extraction has gradually shifted to scheelite. However, separating the associated minerals(e.g., apatite, fluorite, and calcite) and scheelite is challenging because their surface physicochemical properties are similar to those of scheelite. Fortunately, researchers have made substantial progress in separating the minerals of scheelite by using depressants. This study reviews the application and inhibition mechanism of inorganic depressants in obtaining tungsten from its calcium-bearing minerals. The application of new organic depressants in obtaining tungsten from its calcium-bearing minerals and the associated mechanisms are also summarized. After an objective assessment of inorganic and organic depressants’ advantages and disadvantages, possible future research directions for inorganic and organic depressants are proposed. Herein, we provide a theoretical basis for developing scheelite flotation depressants.
文摘Hot dry rock(HDR)geothermal energy is a kind of widely distributed clean energy with huge reserves.However,its commercial development has been constrained by reservoir stimulation.In the early stage of HDR geothermal energy development,properly determining spatial distribution patterns of natural fractures in HDR reservoirs can effectively guide reservoir stimulation.This study analyzes the spatial distribution of natural fractures by using FracMan software based on the actual geological data and log data of well M-2 in the Matouying Uplift area,Hebei Province.The fracture parameters are counted and Monte Carlo simulation technique is introduced to optimize the parameters,which makes the natural fracture model more accurate and reliable.Furthermore,this study simulates hydraulic fracturing using the model combined with the actual in-situ stress parameters and the construction scheme.As verified by fitting the changes in simulated wellhead pressure during hydraulic fracturing with the actual wellhead pressure data detected during construction,the methods for natural fracture modeling used in this study are scientific and reasonable.The preliminary prediction results show that the displacement design scheme with a pump displacement of 2.0-3.0 m^(3)/min,4.0-5.5 m^(3)/min and 6-7 m^(3)/min in the early,middle and late stages,respectively,has good fracturing effect.The results of this study can be utilized as a reference for preparing development schemes for HDR reservoirs.
基金supported by the National Natural Science Foundation of China(Grant Nos.51975278 and 52277055)the Qing Lan Project,the Research Fund of the State Key Laboratory of Mechanics and Control of Mechanical Structures(Nanjing University of Aeronautics and Astronautics)under Grant No.MCMS-I-0321G01+2 种基金the Biomedical Engineering Fusion Laboratory of the affiliated Jiangning Hospital of Nanjing Medical University(Grant No.JNYYZXKY202217)the Postgraduate Research&Practice Innovation Program of NUAA(Grant Nos.xcxjh20220114 and xcxjh20220111)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX23_0353).
文摘Ultrasonic peen forming(UPF)is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming methods.Although ultrasonic transducers with longitudinal vibration have been widely studied,they have seldom been incorporated into UPF devices for machining in confined spaces.To meet the requirements of this type of machining,a sandwich-type piezoelectric transducer with coupled longitudinal-flexural vibrational modes is proposed.The basic structure of the transducer is designed to obtain large vibrational amplitudes in both modes.Experimental results obtained with a prototype device demonstrate the feasibility of the proposed transducer.The measured vibrational amplitude for the working face in the longitudinal vibrational mode is 1.0μm,and electrical matching increases this amplitude by 40%.The flexural vibration characteristics of the same prototype transducer are also tested and are found to be slightly smaller than those of longitudinal mode.The resultant working strokes of the UPF impact pins reach 1.7 mm and 1.2 mm in the longitudinal and flexural modes,respectively.The forming capability of the prototype has been evaluated via 15-min machining on standard 2024-T351 aluminum plates.After UPF,an improved surface morphology with lower surface roughness is obtained.The aluminum plate test piece has an apparent upper deformation with an arc height of 0.64 mm.The measured peak value of the compressive residual stress is around 250 MPa,appearing at a depth of 100μm.The proposed longitudinal-flexural hybrid transducer thus provides a high-performance tool for plate peen forming in confined spaces.
文摘BACKGROUND Serum alanine aminotransferase(ALT) levels are often considered a marker to evaluate liver disease and its severity.AIM To investigate the association between ALT levels and all-cause and cause-specific mortality in patients with nonalcoholic fatty liver disease(NAFLD).METHODS The Third National Health and Nutrition Examination Survey(NHANES-Ⅲ) from 1988 to 1994 and NHANES-Ⅲ-related mortality data from 2019 onward were used to obtain the necessary data for the study. NAFLD was defined as hepatic steatosis, as diagnosed by ultrasound, with no other liver diseases. ALT levels were categorized into four groups according to the different recommended upper limits of normal(ULN) in men and women: < 0.5 ULN, 0.5-1 ULN, 1-2 ULN, and ≥ 2 ULN. The hazard ratios for all-cause mortality and cause-specific mortality were analyzed using the Cox proportional hazard model.RESULTS Multivariate logistic regression analysis demonstrated that the odds ratio of NAFLD correlated positively with increased serum ALT levels. In patients with NAFLD, all-cause mortality and cardiovascular mortality were the highest when ALT was < 0.5 ULN, yet cancer-related mortality was the highest when ALT was ≥ 2 ULN. The same results could be found in both men and women. Univariate analysis showed that severe NAFLD with normal ALT levels had the highest allcause and cause-specific mortality, but the difference was not statistically significant after adjustment for age and multivariate factors.CONCLUSION The risk of NAFLD was positively correlated with ALT level, but all-cause and cardiovascular mortality were the highest when ALT was < 0.5 ULN. Regardless of the severity of NAFLD, normal or lower ALT levels were associated with higher mortality than elevated ALT levels. Clinicians should be aware that high ALT levels indicate liver injury, but low ALT levels are associated with a higher risk of death.
基金Supported by Project of Key Research Base of Humanities and Social Sciences,Ministry of Education and Research Center Base for Revolutionary Spirit and Cultural Resources of the Communist Party of China in Zunyi Normal University(22KRIZYPY12)Teaching Content and Curriculum System Reform and Cultivation Project of Zunyi Normal University in 2022(JGPY2022001)+1 种基金Teaching Content and Curriculum System Reform Project of Guizhou Province in 2022(GZJG20220776)Guizhou Provincial Higher Education Institutions Qianbei Modern Mountain High-efficiency Agricultural Scientific and Technological Achievements Transfer and Transformation Service Rural Revitalization Demonstration Base(Qian Jiao Ji[2022]No.067).
文摘Local red culture is an important educational resource.Integrating it into the course of agronomy majors is an important part of cultivating applied talents who grasp and love agriculture in the new era.This paper analyzes the necessity of integrating local red culture into courses of agronomy majors,discusses the current challenges,and puts forward specific recommendations.
基金supported by the National Natural Science Foundation of China(No.U22A20617)the Natural Science Foundation of Hunan Province,China(Nos.2021JJ30664,2022JJ30559,and 2022JJ50131)Environmental Protection Research Project of Hunan Province,China(No.HBKT-2022036).
文摘In the sewage treatment process,facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic circulation.Cyanophycin granule polypeptide(CGP),a recently discovered high value-added biopolymer present in activated sludge,has provided new avenues for the recovery of resources.However,the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus far.This study investigated the synthesis of CGP,polyhydroxyalkanoates(PHA),and alginate-like exopolysaccharides(ALE)in various microbial aggregates under different carbon sources feeding conditions.Our results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding conditions.Furthermore,biofilm was more conducive to CGP accumulation than floc sludge.Compared with glucose and methanol,sodium acetate significantly enriched the CGP synthetase-encoding gene(cphAabundance=~17419),resulting in the highest CGP yield(average 107.1 mg/g MLSS)in both biofilm and floc sludge.This study is the first to reported the characteristic fluorescence of CGP(Ex/Em=~360/450 nm)caused by the aggregated luminescence of arginine on the side chains.Overall,this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.
