Kernel color is an important trait for assessing the commercial and nutritional quality of foxtail millet. Yellow pigment content （YPC） and carotenoid components （lutein and zeaxanthin） of 270 foxtail millet acces...Kernel color is an important trait for assessing the commercial and nutritional quality of foxtail millet. Yellow pigment content （YPC） and carotenoid components （lutein and zeaxanthin） of 270 foxtail millet accessions, including 50 landraces and 220 improved cultivars, from four different eco-regions in China were surveyed using spectrophotometry and high performance liquid chromatography methods. Results indicated that YPC had rich variance, ranging from 1.91 to 28.54 mg kg-1, with an average value of 17.80 mg kg-1. The average YPC of improved cultivars （18.31 mg kg-1） was significantly higher than that of landraces （15.51 mg kg-l）. The YPC in cultivars from the Loess Plateau spring sowing region （LPSSR） was the highest （20.59 mg kg-~）, followed by the North China summer sowing region （NCSSR, 18.25 mg kg-1）, the northeast spring sowing region （NSSR, 17.25 mg kg-1）, and the Inner Mongolia Plateau spring sowing region （IMPSSR, 13.92 mg kg-1）. The variation coefficients of YPC in cultivars from NSSR, LPSSR, and IMPSSR were higher than that from NCSSR. A similar carotenoid profile was also obtained for 270 foxtail millet cultivars. Lutein and zeaxanthin accounted for approximately 55-65% of YPC in accessions. The lutein content was higher than zeaxanthin content in all cultivars. The ratio of lutein to zeaxanthin ranged from 1.51 to 6.06 with an average of 3.34. YPC was positively correlated with lutein （r=0.935, P〈0.01）, zeaxanthin （r=0.808, P〈0.01 ）, and growth duration （t=0.488, P〈0.01 ）, whereas it was negatively correlated with grain protein （t=-0.332, P〈0.01） and 1 000-kernel weight （t=-0.153, P〈0.05）. Our study is useful for screening and selecting cultivars with high levels of yellow pigment and for enhancing phytochemical concentrations in breeding programs.展开更多
Nitric oxide （NO） is a key signaling molecule in different physiological processes of plants, including programmed cell death （PCD）. PCD of tillers plays an important role in surviving which are major components o...Nitric oxide （NO） is a key signaling molecule in different physiological processes of plants, including programmed cell death （PCD）. PCD of tillers plays an important role in surviving which are major components of grain yield. PCD was triggered in wheat leaves of main stem and tillers by NO content under different nitrogen treatments. In wheat, NO could be synthesized endogenously by nitrate reductase （NR）. As an inducible enzyme, NR activity was closely related to substrate concentration. Therefore, different nitrogen levels would change NR activity and NO production. The objective of this study was to determine the effects of NR activity, NO production, and the correlation between them on different tillers growth, development, senescence, and kernel protein content under different nitrogen levels. Field-experiments were conducted in 2009-2011 growing seasons, using two wheat cultivars with different spike-types. Results showed that for main stem and primary tillers, NR activity and NO content reached high level at heading stage, while for secondary tiller, the level of NR activity was low, but NO content was high in the present research. The NO synthesis depending on NR activity in wheat leaves was significant in the early growing stage, but the NO synthesis weakened with the progress of growing period. NO was related to the senescence of wheat leaves, but PCD was more sensitive to marked changes of NO content than NO content itself. N application had marked influence on the aging process of primary tiller, while had little influence on that of main stem and secondary tiller. Moreover, N fertilizer application could increase spike rate and protein content of primary tiller by N fertilizer application.展开更多
基金supported by the Natural Science Foundation of Shandong,China(ZR2014YL021)the earmarked fund for China Agricultural Research System(CARS-06)+1 种基金the Shandong Agricultural Research System Innovation Team,China(SDAIT-14-03)the Key Projects of Science and Technology Innovation of Shandong Academy of Agricultural Sciences,China(2014CXZ-4)
文摘Kernel color is an important trait for assessing the commercial and nutritional quality of foxtail millet. Yellow pigment content （YPC） and carotenoid components （lutein and zeaxanthin） of 270 foxtail millet accessions, including 50 landraces and 220 improved cultivars, from four different eco-regions in China were surveyed using spectrophotometry and high performance liquid chromatography methods. Results indicated that YPC had rich variance, ranging from 1.91 to 28.54 mg kg-1, with an average value of 17.80 mg kg-1. The average YPC of improved cultivars （18.31 mg kg-1） was significantly higher than that of landraces （15.51 mg kg-l）. The YPC in cultivars from the Loess Plateau spring sowing region （LPSSR） was the highest （20.59 mg kg-~）, followed by the North China summer sowing region （NCSSR, 18.25 mg kg-1）, the northeast spring sowing region （NSSR, 17.25 mg kg-1）, and the Inner Mongolia Plateau spring sowing region （IMPSSR, 13.92 mg kg-1）. The variation coefficients of YPC in cultivars from NSSR, LPSSR, and IMPSSR were higher than that from NCSSR. A similar carotenoid profile was also obtained for 270 foxtail millet cultivars. Lutein and zeaxanthin accounted for approximately 55-65% of YPC in accessions. The lutein content was higher than zeaxanthin content in all cultivars. The ratio of lutein to zeaxanthin ranged from 1.51 to 6.06 with an average of 3.34. YPC was positively correlated with lutein （r=0.935, P〈0.01）, zeaxanthin （r=0.808, P〈0.01 ）, and growth duration （t=0.488, P〈0.01 ）, whereas it was negatively correlated with grain protein （t=-0.332, P〈0.01） and 1 000-kernel weight （t=-0.153, P〈0.05）. Our study is useful for screening and selecting cultivars with high levels of yellow pigment and for enhancing phytochemical concentrations in breeding programs.
基金supported by the National Natural Science Foundation of China(31271661, 30871477)the National Basic Research Program of China(2009CB118602)+1 种基金the Special Fund for Agro-Scientific Research in the Public Interest of China(201203100)the National Science and Technology Support Program of China(2012BAD04B05)
文摘Nitric oxide （NO） is a key signaling molecule in different physiological processes of plants, including programmed cell death （PCD）. PCD of tillers plays an important role in surviving which are major components of grain yield. PCD was triggered in wheat leaves of main stem and tillers by NO content under different nitrogen treatments. In wheat, NO could be synthesized endogenously by nitrate reductase （NR）. As an inducible enzyme, NR activity was closely related to substrate concentration. Therefore, different nitrogen levels would change NR activity and NO production. The objective of this study was to determine the effects of NR activity, NO production, and the correlation between them on different tillers growth, development, senescence, and kernel protein content under different nitrogen levels. Field-experiments were conducted in 2009-2011 growing seasons, using two wheat cultivars with different spike-types. Results showed that for main stem and primary tillers, NR activity and NO content reached high level at heading stage, while for secondary tiller, the level of NR activity was low, but NO content was high in the present research. The NO synthesis depending on NR activity in wheat leaves was significant in the early growing stage, but the NO synthesis weakened with the progress of growing period. NO was related to the senescence of wheat leaves, but PCD was more sensitive to marked changes of NO content than NO content itself. N application had marked influence on the aging process of primary tiller, while had little influence on that of main stem and secondary tiller. Moreover, N fertilizer application could increase spike rate and protein content of primary tiller by N fertilizer application.
