The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and resistance.However,it is unclear whether there is a co-regulatory relationship between...The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and resistance.However,it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought environment.In this study,a drought-sensitive,roll-enhanced,and narrow-leaf mutant(renl1)was induced from a semi-rolled leaf mutant(srl1)by ethyl methane sulfonate(EMS),which was obtained from Nipponbare(NPB)through EMS.Map-based cloning and functional validation showed that RENL1 encodes a cellulose synthase,allelic to NRL1/OsCLSD4.The RENL1 mutation resulted in reduced vascular bundles,vesicular cells,cellulose,and hemicellulose contents in cell walls,diminishing the water-holding capacity of leaves.In addition,the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species(ROS)was decreased,leading to an increase in ROS after drought stress.Meanwhile,genetic results showed that RENL1 and SRL1 synergistically regulated cell wall components.Our results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance,and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance,thereby realizing simultaneous improvement of multiple traits in rice.展开更多
目前腹腔镜手术已广泛应用于各类妇科良恶性疾病的诊治。但仍有多达80%的患者在腹腔镜手术后主诉疼痛,需要使用药物等方式缓解疼痛^([1])。目前临床上多采用阿片类等镇痛药物控制腹腔镜手术的术后疼痛,但单一或联合使用阿片类药物存在...目前腹腔镜手术已广泛应用于各类妇科良恶性疾病的诊治。但仍有多达80%的患者在腹腔镜手术后主诉疼痛,需要使用药物等方式缓解疼痛^([1])。目前临床上多采用阿片类等镇痛药物控制腹腔镜手术的术后疼痛,但单一或联合使用阿片类药物存在呼吸抑制、苏醒延迟以及恶心呕吐等不良反应及风险^([2])。为寻求更好更安全的术后镇痛方案,本次研究拟通过随机对照研究,以术后恢复质量评分量表(quality of recovery,QoR-40)作为主要评价工具,探讨罗哌卡因复合右美托咪定行腹横肌平面阻滞对妇科腹腔镜手术患者术后镇痛及术后恢复质量的影响。展开更多
目的探究解毒生肌膏对2型糖尿病大鼠溃疡皮肤微生态平衡的影响。方法将30只清洁级雄性SD大鼠经STZ诱导,以2型糖尿病大鼠背部全层皮肤缺损为模型,随机分为模型组、解毒生肌膏组(主要有全蝎、蜈蚣组成)、银锌皮肤黏膜抑菌霜组。模型组不...目的探究解毒生肌膏对2型糖尿病大鼠溃疡皮肤微生态平衡的影响。方法将30只清洁级雄性SD大鼠经STZ诱导,以2型糖尿病大鼠背部全层皮肤缺损为模型,随机分为模型组、解毒生肌膏组(主要有全蝎、蜈蚣组成)、银锌皮肤黏膜抑菌霜组。模型组不做处理,其余2组分别给予相应药物外敷,每天1次,共21 d。各组分别于干预后3、7、14、21 d Masson染色观察皮肤溃疡修复情况,采用LFT-1红外线敏感温度计检测创面温度,精密pH试纸检测创面pH值。结果解毒生肌膏组第3天、第7天创面组织炎症细胞数量减少,肉芽组织数量、胶原比例增加;解毒生肌膏组第7天pH值低于模型组(P<0.05),创面温度不具有统计学意义(P>0.05)。结论解毒生肌膏可调节创面pH值趋向正常,减轻创面炎症反应,改善创面微生态,从而促进糖尿病溃疡愈合。展开更多
基金supported by the Nanfan Special Project of Chinese Academy of Agricultural Sciences (Grant No. ZDXM2315)the National Natural Science Foundation of China (Grant Nos. 32372125, 31861143006, and 32188102)+2 种基金Special Support Program of Chinese Academy of Agricultural Sciences (Grant NO. NKYCLJ-C-2021-015)Specific Research Fund of the Innovation Platform for Academicians of Hainan Province2023 College Student Innovation and Entrepreneurship Project of Jiangxi Agricultural University, China (Grant No. S202310410095)
文摘The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and resistance.However,it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought environment.In this study,a drought-sensitive,roll-enhanced,and narrow-leaf mutant(renl1)was induced from a semi-rolled leaf mutant(srl1)by ethyl methane sulfonate(EMS),which was obtained from Nipponbare(NPB)through EMS.Map-based cloning and functional validation showed that RENL1 encodes a cellulose synthase,allelic to NRL1/OsCLSD4.The RENL1 mutation resulted in reduced vascular bundles,vesicular cells,cellulose,and hemicellulose contents in cell walls,diminishing the water-holding capacity of leaves.In addition,the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species(ROS)was decreased,leading to an increase in ROS after drought stress.Meanwhile,genetic results showed that RENL1 and SRL1 synergistically regulated cell wall components.Our results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance,and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance,thereby realizing simultaneous improvement of multiple traits in rice.
文摘目前腹腔镜手术已广泛应用于各类妇科良恶性疾病的诊治。但仍有多达80%的患者在腹腔镜手术后主诉疼痛,需要使用药物等方式缓解疼痛^([1])。目前临床上多采用阿片类等镇痛药物控制腹腔镜手术的术后疼痛,但单一或联合使用阿片类药物存在呼吸抑制、苏醒延迟以及恶心呕吐等不良反应及风险^([2])。为寻求更好更安全的术后镇痛方案,本次研究拟通过随机对照研究,以术后恢复质量评分量表(quality of recovery,QoR-40)作为主要评价工具,探讨罗哌卡因复合右美托咪定行腹横肌平面阻滞对妇科腹腔镜手术患者术后镇痛及术后恢复质量的影响。
文摘目的探究解毒生肌膏对2型糖尿病大鼠溃疡皮肤微生态平衡的影响。方法将30只清洁级雄性SD大鼠经STZ诱导,以2型糖尿病大鼠背部全层皮肤缺损为模型,随机分为模型组、解毒生肌膏组(主要有全蝎、蜈蚣组成)、银锌皮肤黏膜抑菌霜组。模型组不做处理,其余2组分别给予相应药物外敷,每天1次,共21 d。各组分别于干预后3、7、14、21 d Masson染色观察皮肤溃疡修复情况,采用LFT-1红外线敏感温度计检测创面温度,精密pH试纸检测创面pH值。结果解毒生肌膏组第3天、第7天创面组织炎症细胞数量减少,肉芽组织数量、胶原比例增加;解毒生肌膏组第7天pH值低于模型组(P<0.05),创面温度不具有统计学意义(P>0.05)。结论解毒生肌膏可调节创面pH值趋向正常,减轻创面炎症反应,改善创面微生态,从而促进糖尿病溃疡愈合。
