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成年和幼年大鼠海马微环境星形胶质细胞对神经干细胞增殖的作用
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作者 程咏波 渠佳桦 +5 位作者 单闻 刘倩倩 秦建兵 田美玲 张新化 施炜 《解剖学报》 CAS CSCD 北大核心 2023年第4期375-382,共8页
目的 探讨成年和幼年大鼠海马微环境中星形胶质细胞对神经干细胞(NSCs)增殖的作用及其机制。方法 分别取出生后1 d及30周的雌性SD大鼠各5只,分离培养海马星形胶质细胞;孕15 d SD大鼠1只分离培养胚胎海马NSCs;收取星形胶质细胞条件培养基... 目的 探讨成年和幼年大鼠海马微环境中星形胶质细胞对神经干细胞(NSCs)增殖的作用及其机制。方法 分别取出生后1 d及30周的雌性SD大鼠各5只,分离培养海马星形胶质细胞;孕15 d SD大鼠1只分离培养胚胎海马NSCs;收取星形胶质细胞条件培养基(CM)用于培养NSCs。应用流式细胞术和CCK-8检测星形胶质细胞CM培养下NSCs增殖情况;收取星形胶质细胞CM进行质谱分析筛选神经发生相关的差异表达蛋白集落刺激因子1 (CSF-1);应用Western blotting和ELISA验证质谱结果;应用免疫荧光、流式细胞术和CCK-8等技术检测不同浓度CSF-1重组蛋白(20μg/L、100μg/L、1 mg/L及5 mg/L)处理后NSCs增殖情况。结果 与成年组海马星形胶质细胞CM相比,幼年组星形胶质细胞CM可促进NSCs增殖(P<0.01);与幼年组海马星形胶质细胞CM相比,成年组海马中CSF-1表达明显上调(P<0.01);20μg/L CSF-1可促进NSCs增殖(P<0.01),5 mg/L CSF-1明显抑制NSCs增殖(P<0.01)。结论 随着年龄增长,海马微环境中星形胶质细胞分泌CSF-1增加,可调控NSCs增殖。 展开更多
关键词 集落刺激因子1 海马 星形胶质细胞 神经干细胞 免疫荧光 大鼠
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Study on the Impact Factors of COD Throughput in Corncob Extract 被引量:1
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作者 ZHANG Hong-can WANG Qing-liang +3 位作者 HU E-ming cheng yong-bo ZHANG Qi WANG Sha 《Meteorological and Environmental Research》 CAS 2012年第8期32-35,共4页
[ Objectivel The study aims to discuss the impact factors of COD (chemical oxygen demand) throughput in corncob extract. [ Method ] Firstly, as the variations of the mass concentration of corncob, temperature, time ... [ Objectivel The study aims to discuss the impact factors of COD (chemical oxygen demand) throughput in corncob extract. [ Method ] Firstly, as the variations of the mass concentration of corncob, temperature, time and pH, the throughput of COD in corncob extract were analyzed respectively. Afterwards, through the orthogonal test, the best conditions for extracting organic matter from corncob were determined. [ Result] The single-factor tests showed that there was a linear relationship between the mass concentration of corncob and COD of corncob extract when the mass concentration of the corncob was lower than 50 g/L; COD of corncob extract was higher under acidic or alkaline conditions. The optimum con- ditions for extracting organic compounds from corncob were as follows: temperature was 35 ℃, mass concentration was 40 g/L, pH =3, and ex- tracting time was 3 hours; under this conditions, the mass of COD released from a unit mass of corncob reached 0.056 2 g. [ Conclusien] The re- search could decrease the treatment cost of wastewater from uranium mining in mines and make corncob more useful. 展开更多
关键词 CORNCOB Chemical oxygen demand (COD) EXTRACT In situ leaching URANIUM China
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