磁参数法用于评估NiCr基合金热喷涂层结合强度的研究

本文提出了一种基于饱和磁滞回线特征参数的热喷涂层与基材结合强度的无损测试方法。采用MA-WF-05型磁滞多参数评估系统,对在#45碳钢基材上制备的系列NiCr基合金涂层进行了测试,同时对上述样品采用拉伸试验方法测试了结合强度。并通过多元回归分析方法建立了涂层结合强度的预测方程。结果表明预测值与拉伸试验测试值的平均偏差为3.25%,该方法有望推广应用于其他热喷涂层样品。

Identification of EML4 as a key hub gene for endometriosis and its molecular mechanism and potential drug prediction based on the GEO database

Objective:Key genes were screened to analyze molecular mechanisms and their drug targets of endometriosis by applying a bioinformatics approach.Methods:Gene expression profiles of endometriosis and healthy controls were obtained from the Gene Expression Omnibus database.Significant differentially expressed genes were screened using the limma package.Correlation pathways were screened by Spearman correlation analysis on the echinoderm microtubule-associated protein-like 4(EML4)and enrichment in endometriosis pathways and estimated by the GSVA package.Immune characteristics were assessed by the“ESTIMATE”R package.Potential regulatory pathways were determined by enrichment analysis.The SWISS-MODE website was used in homology modeling with EML4 and EML4 protein activity was predicted.VarElect was employed in molecular docking for screening potential compound inhibitors targeting endometriosis.Results:Ten endometriosis and 10 normal samples were included.EML4 was significantly upregulated in endometriosis(p<0.05).Thirty significantly correlated pathways involving 18 positive and 12 negative correlations,including GLYCOSAMINOGLYCAN_BIOSYNTHESIS_HEPARAN_SULFATE and GLYCOSPHINGOLIPID_BIOSYNTHESIS_GANGLIO_SERIES were screened between EML4 and endometriosis.Immunocorrelation analysis showed a significant difference in immune-related pathways in endometriosis and normal samples(p<0.05).In endometriosis,EML4 was associated with T-cell CD4 resting memory,activated mast cells,plasma cells,activated NK cells,M2 macrophages,and follicular helper T cells(p<0.05).Molecular docking identified five potential inhibitors of EML4,and compound DB05104(asimadoline)bound well to EML4 protein to exert its physiological effects.Conclusion:Differential gene expression and immune correlation analyses revealed that EML4 may affect endometriosis through multiple targets and pathways,the mechanism of which involved immune cell activation and infiltration.Molecular docking and dynamics simulation verified DB05104 as a potential inhibitor of EML4 and a powerful target for endometriosis treatment.

Preventing autosomal-dominant hearing loss in Bth mice with CRISPR/CasRx-based RNA editing

CRISPR/RfxCas13d(CasRx)editing system can specifically and precisely cleave single-strand RNAs,which is a promising treatment for various disorders by downregulation of related gene expression.Here,we tested this RNA-editing approach on Beethoven(Bth)mice,an animal model for human DFNA36 due to a point mutation in Tmc1.We first screened 30 sgRNAs in cell cultures and found that CasRx with sgRNA3 reduced the Tmc1^(Bth)transcript by 90.8%,and the Tmc1 wild type transcript(Tmc1^(+))by 44.3%.We then injected a newly developed AAV vector(AAV-PHP.eB)based CasRx into the inner ears of neonatal Bth mice,and we found that Tmc1^(Bth)was reduced by 70.2%in 2 weeks with few off-target effects in the whole transcriptome.Consistently,we found improved hair cell survival,rescued hair bundle degeneration,and reduced mechanoelectrical transduction current.Importantly,the hearing performance,measured in both ABR and DPOAE thresholds,was improved significantly in all ages over 8 weeks.We,therefore,have validated the CRISPR/CasRx-based RNA editing strategy in treating autosomal-dominant hearing loss,paving way for its further application in many other hereditary diseases in hearing and beyond.

Ca^(2+) and OH^- release of ceramsites containing anorthite and gehlenite prepared from waste lime mud

Lime mud is a kind of solid waste in the papermaking industry,which has been a source of serious environmental pollution.Ceramsites containing anorthite and gehlenite were prepared from lime mud and fly ash through the solid state reaction method at 1050°C.The objective of this study was to explore the efficiency of Ca^2＋ and OH-release and assess the phosphorus and copper ion removal performance of the ceramsites via batch experiments,X-ray diffraction（XRD） and scanning electron microscopy（SEM）.The results show that Ca^2＋ and OH-were released from the ceramsites due to the dissolution of anorthite,gehlenite and available lime.It is also concluded that gehlenite had stronger capacity for Ca^2＋ and OH-release compared with anorthite.The Ca^2＋ release could be fit well by the Avrami kinetic model.Increases of porosity,dosage and temperature were associated with increases in the concentrations of Ca^2＋ and OH-released.Under different conditions,the ceramsites could maintain aqueous solutions in alkaline conditions（p H = 9.3–10.9） and the release of Ca^2＋ was not affected.The removal rates of phosphorus and copper ions were as high as 96.88% and 96.81%,respectively.The final p H values of both phosphorus and copper ions solutions changed slightly.The reuse of lime mud in the form of ceramsites is an effective strategy.